B7-H1-deficiency enhances the potential of tolerogenic dendritic cells by activating CD1d-restricted type II NKT cells.

<h4>Background</h4>Dendritic cells (DC) can act tolerogenic at a semi-mature stage by induction of protective CD4(+) T cell and NKT cell responses.<h4>Methodology/principal findings</h4>Here we studied the role of the co-inhibitory molecule B7-H1 (PD-L1, CD274) on semi-mature...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Carolin Brandl, Sonja Ortler, Thomas Herrmann, Susanna Cardell, Manfred B Lutz, Heinz Wiendl
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b31f1ec16bec437daa228ad4988f7213
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:b31f1ec16bec437daa228ad4988f7213
record_format	dspace
spelling	oai:doaj.org-article:b31f1ec16bec437daa228ad4988f72132021-12-02T20:21:25ZB7-H1-deficiency enhances the potential of tolerogenic dendritic cells by activating CD1d-restricted type II NKT cells.1932-620310.1371/journal.pone.0010800https://doaj.org/article/b31f1ec16bec437daa228ad4988f72132010-05-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20520738/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Dendritic cells (DC) can act tolerogenic at a semi-mature stage by induction of protective CD4(+) T cell and NKT cell responses.<h4>Methodology/principal findings</h4>Here we studied the role of the co-inhibitory molecule B7-H1 (PD-L1, CD274) on semi-mature DC that were generated from bone marrow (BM) cells of B7-H1(-/-) mice and applied to the model of Experimental Autoimmune Encephalomyelitis (EAE). Injections of B7-H1-deficient DC showed increased EAE protection as compared to wild type (WT)-DC. Injections of B7-H1(-/-) TNF-DC induced higher release of peptide-specific IL-10 and IL-13 after restimulation in vitro together with elevated serum cytokines IL-4 and IL-13 produced by NKT cells, and reduced IL-17 and IFN-gamma production in the CNS. Experiments in CD1d(-/-) and Jalpha281(-/-) mice as well as with type I and II NKT cell lines indicated that only type II NKT cells but not type I NKT cells (invariant NKT cells) could be stimulated by an endogenous CD1d-ligand on DC and were responsible for the increased serum cytokine production in the absence of B7-H1.<h4>Conclusions/significance</h4>Together, our data indicate that BM-DC express an endogenous CD1d ligand and B7-H1 to ihibit type II but not type I NKT cells. In the absence of B7-H1 on these DC their tolerogenic potential to stimulate tolerogenic CD4(+) and NKT cell responses is enhanced.Carolin BrandlSonja OrtlerThomas HerrmannSusanna CardellManfred B LutzHeinz WiendlPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 5, p e10800 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Carolin Brandl Sonja Ortler Thomas Herrmann Susanna Cardell Manfred B Lutz Heinz Wiendl B7-H1-deficiency enhances the potential of tolerogenic dendritic cells by activating CD1d-restricted type II NKT cells.
description	<h4>Background</h4>Dendritic cells (DC) can act tolerogenic at a semi-mature stage by induction of protective CD4(+) T cell and NKT cell responses.<h4>Methodology/principal findings</h4>Here we studied the role of the co-inhibitory molecule B7-H1 (PD-L1, CD274) on semi-mature DC that were generated from bone marrow (BM) cells of B7-H1(-/-) mice and applied to the model of Experimental Autoimmune Encephalomyelitis (EAE). Injections of B7-H1-deficient DC showed increased EAE protection as compared to wild type (WT)-DC. Injections of B7-H1(-/-) TNF-DC induced higher release of peptide-specific IL-10 and IL-13 after restimulation in vitro together with elevated serum cytokines IL-4 and IL-13 produced by NKT cells, and reduced IL-17 and IFN-gamma production in the CNS. Experiments in CD1d(-/-) and Jalpha281(-/-) mice as well as with type I and II NKT cell lines indicated that only type II NKT cells but not type I NKT cells (invariant NKT cells) could be stimulated by an endogenous CD1d-ligand on DC and were responsible for the increased serum cytokine production in the absence of B7-H1.<h4>Conclusions/significance</h4>Together, our data indicate that BM-DC express an endogenous CD1d ligand and B7-H1 to ihibit type II but not type I NKT cells. In the absence of B7-H1 on these DC their tolerogenic potential to stimulate tolerogenic CD4(+) and NKT cell responses is enhanced.
format	article
author	Carolin Brandl Sonja Ortler Thomas Herrmann Susanna Cardell Manfred B Lutz Heinz Wiendl
author_facet	Carolin Brandl Sonja Ortler Thomas Herrmann Susanna Cardell Manfred B Lutz Heinz Wiendl
author_sort	Carolin Brandl
title	B7-H1-deficiency enhances the potential of tolerogenic dendritic cells by activating CD1d-restricted type II NKT cells.
title_short	B7-H1-deficiency enhances the potential of tolerogenic dendritic cells by activating CD1d-restricted type II NKT cells.
title_full	B7-H1-deficiency enhances the potential of tolerogenic dendritic cells by activating CD1d-restricted type II NKT cells.
title_fullStr	B7-H1-deficiency enhances the potential of tolerogenic dendritic cells by activating CD1d-restricted type II NKT cells.
title_full_unstemmed	B7-H1-deficiency enhances the potential of tolerogenic dendritic cells by activating CD1d-restricted type II NKT cells.
title_sort	b7-h1-deficiency enhances the potential of tolerogenic dendritic cells by activating cd1d-restricted type ii nkt cells.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/b31f1ec16bec437daa228ad4988f7213
work_keys_str_mv	AT carolinbrandl b7h1deficiencyenhancesthepotentialoftolerogenicdendriticcellsbyactivatingcd1drestrictedtypeiinktcells AT sonjaortler b7h1deficiencyenhancesthepotentialoftolerogenicdendriticcellsbyactivatingcd1drestrictedtypeiinktcells AT thomasherrmann b7h1deficiencyenhancesthepotentialoftolerogenicdendriticcellsbyactivatingcd1drestrictedtypeiinktcells AT susannacardell b7h1deficiencyenhancesthepotentialoftolerogenicdendriticcellsbyactivatingcd1drestrictedtypeiinktcells AT manfredblutz b7h1deficiencyenhancesthepotentialoftolerogenicdendriticcellsbyactivatingcd1drestrictedtypeiinktcells AT heinzwiendl b7h1deficiencyenhancesthepotentialoftolerogenicdendriticcellsbyactivatingcd1drestrictedtypeiinktcells
_version_	1718374125878116352

B7-H1-deficiency enhances the potential of tolerogenic dendritic cells by activating CD1d-restricted type II NKT cells.

Ejemplares similares