Circulating Tumor Cells Count and Morphological Features in Breast, Colorectal and Prostate Cancer.

<h4>Background</h4>Presence of circulating tumor cells (CTC) in patients with metastatic breast, colorectal and prostate cancer is indicative for poor prognosis. An automated CTC (aCTC) algorithm developed previously to eliminate the variability in manual counting of CTC (mCTC) was used...

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Autores principales:	Sjoerd T Ligthart, Frank A W Coumans, Francois-Clement Bidard, Lieke H J Simkens, Cornelis J A Punt, Marco R de Groot, Gerhardt Attard, Johann S de Bono, Jean-Yves Pierga, Leon W M M Terstappen
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b320d45a94964a0986779d1c7671c4e5
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spelling	oai:doaj.org-article:b320d45a94964a0986779d1c7671c4e52021-11-18T07:39:45ZCirculating Tumor Cells Count and Morphological Features in Breast, Colorectal and Prostate Cancer.1932-620310.1371/journal.pone.0067148https://doaj.org/article/b320d45a94964a0986779d1c7671c4e52013-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0067148https://doaj.org/toc/1932-6203<h4>Background</h4>Presence of circulating tumor cells (CTC) in patients with metastatic breast, colorectal and prostate cancer is indicative for poor prognosis. An automated CTC (aCTC) algorithm developed previously to eliminate the variability in manual counting of CTC (mCTC) was used to extract morphological features. Here we validated the aCTC algorithm on CTC images from prostate, breast and colorectal cancer patients and investigated the role of quantitative morphological parameters.<h4>Methodology</h4>Stored images of samples from patients with prostate, breast and colorectal cancer, healthy controls, benign breast and colorectal tumors were obtained using the CellSearch system. Images were analyzed for the presence of aCTC and their morphological parameters measured and correlated with survival.<h4>Results</h4>Overall survival hazard ratio was not significantly different for aCTC and mCTC. The number of CTC correlated strongest with survival, whereas CTC size, roundness and apoptosis features reached significance in univariate analysis, but not in multivariate analysis. One aCTC/7.5 ml of blood was found in 7 of 204 healthy controls and 9 of 694 benign tumors. In one patient with benign tumor 2 and another 9 aCTC were detected.<h4>Significance of the study</h4>CTC can be identified and morphological features extracted by an algorithm on images stored by the CellSearch system and strongly correlate with clinical outcome in metastatic breast, colorectal and prostate cancer.Sjoerd T LigthartFrank A W CoumansFrancois-Clement BidardLieke H J SimkensCornelis J A PuntMarco R de GrootGerhardt AttardJohann S de BonoJean-Yves PiergaLeon W M M TerstappenPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 6, p e67148 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sjoerd T Ligthart Frank A W Coumans Francois-Clement Bidard Lieke H J Simkens Cornelis J A Punt Marco R de Groot Gerhardt Attard Johann S de Bono Jean-Yves Pierga Leon W M M Terstappen Circulating Tumor Cells Count and Morphological Features in Breast, Colorectal and Prostate Cancer.
description	<h4>Background</h4>Presence of circulating tumor cells (CTC) in patients with metastatic breast, colorectal and prostate cancer is indicative for poor prognosis. An automated CTC (aCTC) algorithm developed previously to eliminate the variability in manual counting of CTC (mCTC) was used to extract morphological features. Here we validated the aCTC algorithm on CTC images from prostate, breast and colorectal cancer patients and investigated the role of quantitative morphological parameters.<h4>Methodology</h4>Stored images of samples from patients with prostate, breast and colorectal cancer, healthy controls, benign breast and colorectal tumors were obtained using the CellSearch system. Images were analyzed for the presence of aCTC and their morphological parameters measured and correlated with survival.<h4>Results</h4>Overall survival hazard ratio was not significantly different for aCTC and mCTC. The number of CTC correlated strongest with survival, whereas CTC size, roundness and apoptosis features reached significance in univariate analysis, but not in multivariate analysis. One aCTC/7.5 ml of blood was found in 7 of 204 healthy controls and 9 of 694 benign tumors. In one patient with benign tumor 2 and another 9 aCTC were detected.<h4>Significance of the study</h4>CTC can be identified and morphological features extracted by an algorithm on images stored by the CellSearch system and strongly correlate with clinical outcome in metastatic breast, colorectal and prostate cancer.
format	article
author	Sjoerd T Ligthart Frank A W Coumans Francois-Clement Bidard Lieke H J Simkens Cornelis J A Punt Marco R de Groot Gerhardt Attard Johann S de Bono Jean-Yves Pierga Leon W M M Terstappen
author_facet	Sjoerd T Ligthart Frank A W Coumans Francois-Clement Bidard Lieke H J Simkens Cornelis J A Punt Marco R de Groot Gerhardt Attard Johann S de Bono Jean-Yves Pierga Leon W M M Terstappen
author_sort	Sjoerd T Ligthart
title	Circulating Tumor Cells Count and Morphological Features in Breast, Colorectal and Prostate Cancer.
title_short	Circulating Tumor Cells Count and Morphological Features in Breast, Colorectal and Prostate Cancer.
title_full	Circulating Tumor Cells Count and Morphological Features in Breast, Colorectal and Prostate Cancer.
title_fullStr	Circulating Tumor Cells Count and Morphological Features in Breast, Colorectal and Prostate Cancer.
title_full_unstemmed	Circulating Tumor Cells Count and Morphological Features in Breast, Colorectal and Prostate Cancer.
title_sort	circulating tumor cells count and morphological features in breast, colorectal and prostate cancer.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/b320d45a94964a0986779d1c7671c4e5
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Circulating Tumor Cells Count and Morphological Features in Breast, Colorectal and Prostate Cancer.

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