Differential adaptability between reference strains and clinical isolates of Pseudomonas aeruginosa into the lung epithelium intracellular lifestyle

Intracellular invasion is an advantageous mechanism used by pathogens to evade host defense and antimicrobial therapy. In patients, the intracellular microbial lifestyle can lead to infection persistence and recurrence, thus worsening outcomes. Lung infections caused by Pseudomonas aeruginosa, espec...

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Autores principales:	Maria Del Mar Cendra, Eduard Torrents
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2020
Materias:	pseudomonas aeruginosa intracellular persistence lung epithelial cells clinical isolates host-pathogen interactions intracellular lifestyle chronic infections cystic fibrosis ribonucleotide reductase Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/b3229216cdc840e986ff372a92723ac1
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spelling	oai:doaj.org-article:b3229216cdc840e986ff372a92723ac12021-11-17T14:21:58ZDifferential adaptability between reference strains and clinical isolates of Pseudomonas aeruginosa into the lung epithelium intracellular lifestyle2150-55942150-560810.1080/21505594.2020.1787034https://doaj.org/article/b3229216cdc840e986ff372a92723ac12020-12-01T00:00:00Zhttp://dx.doi.org/10.1080/21505594.2020.1787034https://doaj.org/toc/2150-5594https://doaj.org/toc/2150-5608Intracellular invasion is an advantageous mechanism used by pathogens to evade host defense and antimicrobial therapy. In patients, the intracellular microbial lifestyle can lead to infection persistence and recurrence, thus worsening outcomes. Lung infections caused by Pseudomonas aeruginosa, especially in cystic fibrosis (CF) patients, are often aggravated by intracellular invasion and persistence of the pathogen. Proliferation of the infectious species relies on a continuous deoxyribonucleotide (dNTP) supply, for which the ribonucleotide reductase enzyme (RNR) is the unique provider. The large genome plasticity of P. aeruginosa and its ability to rapidly adapt to different environments are challenges for studying the pathophysiology associated with this type of infection. Using different reference strains and clinical isolates of P. aeruginosa independently combined with alveolar (A549) and bronchial (16HBE14o- and CF-CFBE41o-) epithelial cells, we analyzed host–pathogen interactions and intracellular bacterial persistence with the aim of determining a cell type-directed infection promoted by the P. aeruginosa strains. The oscillations in cellular toxicity and oxygen consumption promoted by the intracellular persistence of the strains were also analyzed among the different infectious lung models. Significantly, we identified class II RNR as the enzyme that supplies dNTPs to intracellular P. aeruginosa. This discovery could contribute to the development of RNR-targeted strategies against the chronicity occurring in this type of lung infection. Overall our study demonstrates that the choice of bacterial strain is critical to properly study the type of infectious process with relevant translational outcomes.Maria Del Mar CendraEduard TorrentsTaylor & Francis Grouparticlepseudomonas aeruginosaintracellular persistencelungepithelial cellsclinical isolateshost-pathogen interactionsintracellular lifestylechronic infectionscystic fibrosisribonucleotide reductaseInfectious and parasitic diseasesRC109-216ENVirulence, Vol 11, Iss 1, Pp 862-876 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	pseudomonas aeruginosa intracellular persistence lung epithelial cells clinical isolates host-pathogen interactions intracellular lifestyle chronic infections cystic fibrosis ribonucleotide reductase Infectious and parasitic diseases RC109-216
spellingShingle	pseudomonas aeruginosa intracellular persistence lung epithelial cells clinical isolates host-pathogen interactions intracellular lifestyle chronic infections cystic fibrosis ribonucleotide reductase Infectious and parasitic diseases RC109-216 Maria Del Mar Cendra Eduard Torrents Differential adaptability between reference strains and clinical isolates of Pseudomonas aeruginosa into the lung epithelium intracellular lifestyle
description	Intracellular invasion is an advantageous mechanism used by pathogens to evade host defense and antimicrobial therapy. In patients, the intracellular microbial lifestyle can lead to infection persistence and recurrence, thus worsening outcomes. Lung infections caused by Pseudomonas aeruginosa, especially in cystic fibrosis (CF) patients, are often aggravated by intracellular invasion and persistence of the pathogen. Proliferation of the infectious species relies on a continuous deoxyribonucleotide (dNTP) supply, for which the ribonucleotide reductase enzyme (RNR) is the unique provider. The large genome plasticity of P. aeruginosa and its ability to rapidly adapt to different environments are challenges for studying the pathophysiology associated with this type of infection. Using different reference strains and clinical isolates of P. aeruginosa independently combined with alveolar (A549) and bronchial (16HBE14o- and CF-CFBE41o-) epithelial cells, we analyzed host–pathogen interactions and intracellular bacterial persistence with the aim of determining a cell type-directed infection promoted by the P. aeruginosa strains. The oscillations in cellular toxicity and oxygen consumption promoted by the intracellular persistence of the strains were also analyzed among the different infectious lung models. Significantly, we identified class II RNR as the enzyme that supplies dNTPs to intracellular P. aeruginosa. This discovery could contribute to the development of RNR-targeted strategies against the chronicity occurring in this type of lung infection. Overall our study demonstrates that the choice of bacterial strain is critical to properly study the type of infectious process with relevant translational outcomes.
format	article
author	Maria Del Mar Cendra Eduard Torrents
author_facet	Maria Del Mar Cendra Eduard Torrents
author_sort	Maria Del Mar Cendra
title	Differential adaptability between reference strains and clinical isolates of Pseudomonas aeruginosa into the lung epithelium intracellular lifestyle
title_short	Differential adaptability between reference strains and clinical isolates of Pseudomonas aeruginosa into the lung epithelium intracellular lifestyle
title_full	Differential adaptability between reference strains and clinical isolates of Pseudomonas aeruginosa into the lung epithelium intracellular lifestyle
title_fullStr	Differential adaptability between reference strains and clinical isolates of Pseudomonas aeruginosa into the lung epithelium intracellular lifestyle
title_full_unstemmed	Differential adaptability between reference strains and clinical isolates of Pseudomonas aeruginosa into the lung epithelium intracellular lifestyle
title_sort	differential adaptability between reference strains and clinical isolates of pseudomonas aeruginosa into the lung epithelium intracellular lifestyle
publisher	Taylor & Francis Group
publishDate	2020
url	https://doaj.org/article/b3229216cdc840e986ff372a92723ac1
work_keys_str_mv	AT mariadelmarcendra differentialadaptabilitybetweenreferencestrainsandclinicalisolatesofpseudomonasaeruginosaintothelungepitheliumintracellularlifestyle AT eduardtorrents differentialadaptabilitybetweenreferencestrainsandclinicalisolatesofpseudomonasaeruginosaintothelungepitheliumintracellularlifestyle
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Differential adaptability between reference strains and clinical isolates of Pseudomonas aeruginosa into the lung epithelium intracellular lifestyle

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