Biosynthesis of Silver Nanoparticles from Melia azedarach: Enhancement of Antibacterial, Wound Healing, Antidiabetic and Antioxidant Activities

Gandhimathi Chinnasamy, Smitha Chandrasekharan, Somika Bhatnagar Plant Transformation and Tissue Culture, Temasek Life Sciences Laboratory, National University of Singapore, 117604, SingaporeCorrespondence: Somika BhatnagarPlant Transformation and Tissue Culture, Temasek Life Sciences Laboratory, 1...

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Autores principales:	Chinnasamy G, Chandrasekharan S, Bhatnagar S
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	medicinal plants phytochemicals nanotechnology green synthesis Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/b32494b4c5334d128b053b075a15fc4d
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spelling	oai:doaj.org-article:b32494b4c5334d128b053b075a15fc4d2021-12-02T09:16:28ZBiosynthesis of Silver Nanoparticles from Melia azedarach: Enhancement of Antibacterial, Wound Healing, Antidiabetic and Antioxidant Activities1178-2013https://doaj.org/article/b32494b4c5334d128b053b075a15fc4d2019-12-01T00:00:00Zhttps://www.dovepress.com/biosynthesis-of-silver-nanoparticles-from-melia-azedarach-enhancement--peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Gandhimathi Chinnasamy, Smitha Chandrasekharan, Somika Bhatnagar Plant Transformation and Tissue Culture, Temasek Life Sciences Laboratory, National University of Singapore, 117604, SingaporeCorrespondence: Somika BhatnagarPlant Transformation and Tissue Culture, Temasek Life Sciences Laboratory, 1 Research Link, National University of Singapore, 117604, SingaporeTel +65-68727630Email somika@tll.org.sgPurpose: Global demand for novel, biocompatible, eco-friendly resources to fight diseases inspired this study. We investigated plants used in traditional medicine systems and utilized nanotechnology to synthesize, evaluate, and enhance potential applications in nanomedicine.Methods: Aqueous leaf extract from Melia azedarach (MA) was utilized for bio-synthesis of silver nanoparticles (MA-AgNPs). Reaction conditions were optimized for high yield and colloidal stability was evaluated using UV-Vis spectroscopy. MA-AgNPs were characterized by scanning electron microscopy (SEM), energy-dispersive X-ray (EDX), transmission electron microscopy (TEM), X-ray diffraction (XRD), and Fourier transform infrared spectroscopy (FTIR). Standard methods were used to analyze the antibacterial, wound healing, antidiabetic, antioxidant, and cytotoxic activities.Results: The formation of MA-AgNPs at room temperature was confirmed by stable brown colloidal solution with maximum absorbance at 420 nm (UV-Vis Spectroscopy). MA-AgNPs were spherical (SEM), uniformly dispersed, 14–20 nm in diameter (TEM), and crystalline in nature (XRD). Presence of elemental silver was confirmed by peak at 3 KeV (EDX). FTIR data revealed the presence of functional groups which indicate phyto-constituents (polyphenols, flavonoids, and terpenoids) may have acted as the reducing and capping agents. MA-AgNPs (1000 μg/mL) showed larger zone of inhibition than MA-extract in the disk diffusion assay for human pathogenic gram positive bacteria, Bacillus cereus (34 mm) and gram negative, Escherichia coli (37 mm), thus confirming their higher antibacterial activity. The cell scratch assay on human dermal fibroblast cells revealed potential wound healing activity. The MA-AgNPs (400 μg/mL) demonstrated high antidiabetic efficacy as measured by α-amylase (85.75%) and α-glucosidase (80.33%) inhibition assays and antioxidant activity as analyzed by DPPH (63.83%) and ABTS (63.61%) radical scavenging assays. Toxic effect of MA-AgNPs against human chang liver cells (CCL-13) as determined by MTS assay, optical microscopic and CMFDA dye methods was insignificant.Conclusion: This sustainable, green synthesis of AgNPs is a competitive alternative to conventional methods and will play a significant role in biomedical applications of Melia azedarach.Keywords: medicinal plants, phytochemicals, nanotechnology, green synthesisChinnasamy GChandrasekharan SBhatnagar SDove Medical Pressarticlemedicinal plantsphytochemicalsnanotechnologygreen synthesisMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 9823-9836 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	medicinal plants phytochemicals nanotechnology green synthesis Medicine (General) R5-920
spellingShingle	medicinal plants phytochemicals nanotechnology green synthesis Medicine (General) R5-920 Chinnasamy G Chandrasekharan S Bhatnagar S Biosynthesis of Silver Nanoparticles from Melia azedarach: Enhancement of Antibacterial, Wound Healing, Antidiabetic and Antioxidant Activities
description	Gandhimathi Chinnasamy, Smitha Chandrasekharan, Somika Bhatnagar Plant Transformation and Tissue Culture, Temasek Life Sciences Laboratory, National University of Singapore, 117604, SingaporeCorrespondence: Somika BhatnagarPlant Transformation and Tissue Culture, Temasek Life Sciences Laboratory, 1 Research Link, National University of Singapore, 117604, SingaporeTel +65-68727630Email somika@tll.org.sgPurpose: Global demand for novel, biocompatible, eco-friendly resources to fight diseases inspired this study. We investigated plants used in traditional medicine systems and utilized nanotechnology to synthesize, evaluate, and enhance potential applications in nanomedicine.Methods: Aqueous leaf extract from Melia azedarach (MA) was utilized for bio-synthesis of silver nanoparticles (MA-AgNPs). Reaction conditions were optimized for high yield and colloidal stability was evaluated using UV-Vis spectroscopy. MA-AgNPs were characterized by scanning electron microscopy (SEM), energy-dispersive X-ray (EDX), transmission electron microscopy (TEM), X-ray diffraction (XRD), and Fourier transform infrared spectroscopy (FTIR). Standard methods were used to analyze the antibacterial, wound healing, antidiabetic, antioxidant, and cytotoxic activities.Results: The formation of MA-AgNPs at room temperature was confirmed by stable brown colloidal solution with maximum absorbance at 420 nm (UV-Vis Spectroscopy). MA-AgNPs were spherical (SEM), uniformly dispersed, 14–20 nm in diameter (TEM), and crystalline in nature (XRD). Presence of elemental silver was confirmed by peak at 3 KeV (EDX). FTIR data revealed the presence of functional groups which indicate phyto-constituents (polyphenols, flavonoids, and terpenoids) may have acted as the reducing and capping agents. MA-AgNPs (1000 μg/mL) showed larger zone of inhibition than MA-extract in the disk diffusion assay for human pathogenic gram positive bacteria, Bacillus cereus (34 mm) and gram negative, Escherichia coli (37 mm), thus confirming their higher antibacterial activity. The cell scratch assay on human dermal fibroblast cells revealed potential wound healing activity. The MA-AgNPs (400 μg/mL) demonstrated high antidiabetic efficacy as measured by α-amylase (85.75%) and α-glucosidase (80.33%) inhibition assays and antioxidant activity as analyzed by DPPH (63.83%) and ABTS (63.61%) radical scavenging assays. Toxic effect of MA-AgNPs against human chang liver cells (CCL-13) as determined by MTS assay, optical microscopic and CMFDA dye methods was insignificant.Conclusion: This sustainable, green synthesis of AgNPs is a competitive alternative to conventional methods and will play a significant role in biomedical applications of Melia azedarach.Keywords: medicinal plants, phytochemicals, nanotechnology, green synthesis
format	article
author	Chinnasamy G Chandrasekharan S Bhatnagar S
author_facet	Chinnasamy G Chandrasekharan S Bhatnagar S
author_sort	Chinnasamy G
title	Biosynthesis of Silver Nanoparticles from Melia azedarach: Enhancement of Antibacterial, Wound Healing, Antidiabetic and Antioxidant Activities
title_short	Biosynthesis of Silver Nanoparticles from Melia azedarach: Enhancement of Antibacterial, Wound Healing, Antidiabetic and Antioxidant Activities
title_full	Biosynthesis of Silver Nanoparticles from Melia azedarach: Enhancement of Antibacterial, Wound Healing, Antidiabetic and Antioxidant Activities
title_fullStr	Biosynthesis of Silver Nanoparticles from Melia azedarach: Enhancement of Antibacterial, Wound Healing, Antidiabetic and Antioxidant Activities
title_full_unstemmed	Biosynthesis of Silver Nanoparticles from Melia azedarach: Enhancement of Antibacterial, Wound Healing, Antidiabetic and Antioxidant Activities
title_sort	biosynthesis of silver nanoparticles from melia azedarach: enhancement of antibacterial, wound healing, antidiabetic and antioxidant activities
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/b32494b4c5334d128b053b075a15fc4d
work_keys_str_mv	AT chinnasamyg biosynthesisofsilvernanoparticlesfrommeliaazedarachenhancementofantibacterialwoundhealingantidiabeticandantioxidantactivities AT chandrasekharans biosynthesisofsilvernanoparticlesfrommeliaazedarachenhancementofantibacterialwoundhealingantidiabeticandantioxidantactivities AT bhatnagars biosynthesisofsilvernanoparticlesfrommeliaazedarachenhancementofantibacterialwoundhealingantidiabeticandantioxidantactivities
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Biosynthesis of Silver Nanoparticles from Melia azedarach: Enhancement of Antibacterial, Wound Healing, Antidiabetic and Antioxidant Activities

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