Developing functional markers for vitamin E biosynthesis in oil palm.

Vitamin E is essential for human health and plays positive roles in anti-oxidation. Previously, we detected large variation in vitamin E content among 161 oil palm accessions. In this study, twenty oil palm accessions with distinct variation in vitamin E contents (171.30 to 1 258.50 ppm) were select...

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Autores principales:	Yajing Dou, Wei Xia, Annaliese S Mason, Dongyi Huang, Xiwei Sun, Haikuo Fan, Yong Xiao
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b324ecaf27fa44fa956ca1e0eef7413f
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spelling	oai:doaj.org-article:b324ecaf27fa44fa956ca1e0eef7413f2021-12-02T20:12:40ZDeveloping functional markers for vitamin E biosynthesis in oil palm.1932-620310.1371/journal.pone.0259684https://doaj.org/article/b324ecaf27fa44fa956ca1e0eef7413f2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0259684https://doaj.org/toc/1932-6203Vitamin E is essential for human health and plays positive roles in anti-oxidation. Previously, we detected large variation in vitamin E content among 161 oil palm accessions. In this study, twenty oil palm accessions with distinct variation in vitamin E contents (171.30 to 1 258.50 ppm) were selected for genetic variation analysis and developing functional markers associated with vitamin E contents. Thirty-seven homologous genes in oil palm belonging to vitamin E biosynthesis pathway were identified via BLASTP analysis, the lengths of which ranged from 426 to 25 717 bp (average 7 089 bp). Multiplex PCR sequencing for the 37 genes found 1 703 SNPs and 85 indels among the 20 oil palm accessions, with 226 SNPs locating in the coding regions. Clustering analysis for these polymorphic loci showed that the 20 oil palm accessions could be divided into five groups. Among these groups, group I included eight oil palm accessions whose vitamin E content (mean value: 893.50 ppm) was far higher than other groups (mean value 256.29 to 532.94 ppm). Correlation analysis between the markers and vitamin E traits showed that 134 SNP and 7 indel markers were significantly (p < 0.05) related with total vitamin E content. Among these functional markers, the indel EgTMT-1-24 was highly correlated with variation in vitamin E content, especially tocotrienol content. Our study identified a number of candidate function associated markers and provided clues for further research into molecular breeding for high vitamin E content oil palm.Yajing DouWei XiaAnnaliese S MasonDongyi HuangXiwei SunHaikuo FanYong XiaoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 11, p e0259684 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yajing Dou Wei Xia Annaliese S Mason Dongyi Huang Xiwei Sun Haikuo Fan Yong Xiao Developing functional markers for vitamin E biosynthesis in oil palm.
description	Vitamin E is essential for human health and plays positive roles in anti-oxidation. Previously, we detected large variation in vitamin E content among 161 oil palm accessions. In this study, twenty oil palm accessions with distinct variation in vitamin E contents (171.30 to 1 258.50 ppm) were selected for genetic variation analysis and developing functional markers associated with vitamin E contents. Thirty-seven homologous genes in oil palm belonging to vitamin E biosynthesis pathway were identified via BLASTP analysis, the lengths of which ranged from 426 to 25 717 bp (average 7 089 bp). Multiplex PCR sequencing for the 37 genes found 1 703 SNPs and 85 indels among the 20 oil palm accessions, with 226 SNPs locating in the coding regions. Clustering analysis for these polymorphic loci showed that the 20 oil palm accessions could be divided into five groups. Among these groups, group I included eight oil palm accessions whose vitamin E content (mean value: 893.50 ppm) was far higher than other groups (mean value 256.29 to 532.94 ppm). Correlation analysis between the markers and vitamin E traits showed that 134 SNP and 7 indel markers were significantly (p < 0.05) related with total vitamin E content. Among these functional markers, the indel EgTMT-1-24 was highly correlated with variation in vitamin E content, especially tocotrienol content. Our study identified a number of candidate function associated markers and provided clues for further research into molecular breeding for high vitamin E content oil palm.
format	article
author	Yajing Dou Wei Xia Annaliese S Mason Dongyi Huang Xiwei Sun Haikuo Fan Yong Xiao
author_facet	Yajing Dou Wei Xia Annaliese S Mason Dongyi Huang Xiwei Sun Haikuo Fan Yong Xiao
author_sort	Yajing Dou
title	Developing functional markers for vitamin E biosynthesis in oil palm.
title_short	Developing functional markers for vitamin E biosynthesis in oil palm.
title_full	Developing functional markers for vitamin E biosynthesis in oil palm.
title_fullStr	Developing functional markers for vitamin E biosynthesis in oil palm.
title_full_unstemmed	Developing functional markers for vitamin E biosynthesis in oil palm.
title_sort	developing functional markers for vitamin e biosynthesis in oil palm.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/b324ecaf27fa44fa956ca1e0eef7413f
work_keys_str_mv	AT yajingdou developingfunctionalmarkersforvitaminebiosynthesisinoilpalm AT weixia developingfunctionalmarkersforvitaminebiosynthesisinoilpalm AT annaliesesmason developingfunctionalmarkersforvitaminebiosynthesisinoilpalm AT dongyihuang developingfunctionalmarkersforvitaminebiosynthesisinoilpalm AT xiweisun developingfunctionalmarkersforvitaminebiosynthesisinoilpalm AT haikuofan developingfunctionalmarkersforvitaminebiosynthesisinoilpalm AT yongxiao developingfunctionalmarkersforvitaminebiosynthesisinoilpalm
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Developing functional markers for vitamin E biosynthesis in oil palm.

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