Capsule type of Streptococcus pneumoniae determines growth phenotype.

The polysaccharide capsule of Streptococcus pneumoniae defines over ninety serotypes, which differ in their carriage prevalence and invasiveness for poorly understood reasons. Recently, an inverse correlation between carriage prevalence and oligosaccharide structure of a given capsule has been descr...

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Autores principales:	Lucy J Hathaway, Silvio D Brugger, Brigitte Morand, Mathieu Bangert, Jeannine U Rotzetter, Christoph Hauser, Werner A Graber, Suzanna Gore, Aras Kadioglu, Kathrin Mühlemann
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/b32bdc890c3545e6bcd7c929e3a8c36f
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spelling	oai:doaj.org-article:b32bdc890c3545e6bcd7c929e3a8c36f2021-11-18T06:04:40ZCapsule type of Streptococcus pneumoniae determines growth phenotype.1553-73661553-737410.1371/journal.ppat.1002574https://doaj.org/article/b32bdc890c3545e6bcd7c929e3a8c36f2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22412375/pdf/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374The polysaccharide capsule of Streptococcus pneumoniae defines over ninety serotypes, which differ in their carriage prevalence and invasiveness for poorly understood reasons. Recently, an inverse correlation between carriage prevalence and oligosaccharide structure of a given capsule has been described. Our previous work suggested a link between serotype and growth in vitro. Here we investigate whether capsule production interferes with growth in vitro and whether this predicts carriage prevalence in vivo. Eighty-one capsule switch mutants were constructed representing nine different serotypes, five of low (4, 7F, 14, 15, 18C) and four of high carriage prevalence (6B, 9V, 19F, 23F). Growth (length of lag phase, maximum optical density) of wildtype strains, nontypeable mutants and capsule switch mutants was studied in nutrient-restricted Lacks medium (MLM) and in rich undefined brain heart infusion broth supplemented with 5% foetal calf serum (BHI+FCS). In MLM growth phenotype depended on, and was transferred with, capsule operon type. Colonization efficiency of mouse nasopharynx also depended on, and was transferred with, capsule operon type. Capsule production interfered with growth, which correlated inversely with serotype-specific carriage prevalence. Serotypes with better growth and higher carriage prevalence produced thicker capsules (by electron microscopy, FITC-dextran exclusion assays and HPLC) than serotypes with delayed growth and low carriage prevalence. However, expression of cpsA, the first capsule gene, (by quantitative RT-PCR) correlated inversely with capsule thickness. Energy spent for capsule production (incorporation of H3-glucose) relative to amount of capsule produced was higher for serotypes with low carriage prevalence. Experiments in BHI+FCS showed overall better bacterial growth and more capsule production than growth in MLM and differences between serotypes were no longer apparent. Production of polysaccharide capsule in S. pneumoniae interferes with growth in nutrient-limiting conditions probably by competition for energy against the central metabolism. Serotype-specific nasopharyngeal carriage prevalence in vivo is predicted by the growth phenotype.Lucy J HathawaySilvio D BruggerBrigitte MorandMathieu BangertJeannine U RotzetterChristoph HauserWerner A GraberSuzanna GoreAras KadiogluKathrin MühlemannPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 8, Iss 3, p e1002574 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Lucy J Hathaway Silvio D Brugger Brigitte Morand Mathieu Bangert Jeannine U Rotzetter Christoph Hauser Werner A Graber Suzanna Gore Aras Kadioglu Kathrin Mühlemann Capsule type of Streptococcus pneumoniae determines growth phenotype.
description	The polysaccharide capsule of Streptococcus pneumoniae defines over ninety serotypes, which differ in their carriage prevalence and invasiveness for poorly understood reasons. Recently, an inverse correlation between carriage prevalence and oligosaccharide structure of a given capsule has been described. Our previous work suggested a link between serotype and growth in vitro. Here we investigate whether capsule production interferes with growth in vitro and whether this predicts carriage prevalence in vivo. Eighty-one capsule switch mutants were constructed representing nine different serotypes, five of low (4, 7F, 14, 15, 18C) and four of high carriage prevalence (6B, 9V, 19F, 23F). Growth (length of lag phase, maximum optical density) of wildtype strains, nontypeable mutants and capsule switch mutants was studied in nutrient-restricted Lacks medium (MLM) and in rich undefined brain heart infusion broth supplemented with 5% foetal calf serum (BHI+FCS). In MLM growth phenotype depended on, and was transferred with, capsule operon type. Colonization efficiency of mouse nasopharynx also depended on, and was transferred with, capsule operon type. Capsule production interfered with growth, which correlated inversely with serotype-specific carriage prevalence. Serotypes with better growth and higher carriage prevalence produced thicker capsules (by electron microscopy, FITC-dextran exclusion assays and HPLC) than serotypes with delayed growth and low carriage prevalence. However, expression of cpsA, the first capsule gene, (by quantitative RT-PCR) correlated inversely with capsule thickness. Energy spent for capsule production (incorporation of H3-glucose) relative to amount of capsule produced was higher for serotypes with low carriage prevalence. Experiments in BHI+FCS showed overall better bacterial growth and more capsule production than growth in MLM and differences between serotypes were no longer apparent. Production of polysaccharide capsule in S. pneumoniae interferes with growth in nutrient-limiting conditions probably by competition for energy against the central metabolism. Serotype-specific nasopharyngeal carriage prevalence in vivo is predicted by the growth phenotype.
format	article
author	Lucy J Hathaway Silvio D Brugger Brigitte Morand Mathieu Bangert Jeannine U Rotzetter Christoph Hauser Werner A Graber Suzanna Gore Aras Kadioglu Kathrin Mühlemann
author_facet	Lucy J Hathaway Silvio D Brugger Brigitte Morand Mathieu Bangert Jeannine U Rotzetter Christoph Hauser Werner A Graber Suzanna Gore Aras Kadioglu Kathrin Mühlemann
author_sort	Lucy J Hathaway
title	Capsule type of Streptococcus pneumoniae determines growth phenotype.
title_short	Capsule type of Streptococcus pneumoniae determines growth phenotype.
title_full	Capsule type of Streptococcus pneumoniae determines growth phenotype.
title_fullStr	Capsule type of Streptococcus pneumoniae determines growth phenotype.
title_full_unstemmed	Capsule type of Streptococcus pneumoniae determines growth phenotype.
title_sort	capsule type of streptococcus pneumoniae determines growth phenotype.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/b32bdc890c3545e6bcd7c929e3a8c36f
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_version_	1718424627619823616

Capsule type of Streptococcus pneumoniae determines growth phenotype.

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