Acclimatization and Field Evaluation of Micropropagated Plants of Chrysanthemum Cv.'Arka Swarna'

Chrysanthemum cv.'Arka Swarna' was micropropagated using shoot-tip and nodal microcuttings on MS medium containing 3% sucrose and 0.25% Phytagel® in the absence of externally supplied plant growth regulators, yielding 90-100% rooted plantlets, or in medium containing 1 μM benzyladenine or...

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Autores principales:	Bindu Panicker, Pious Thomas, T Janakiram
Formato:	article
Lenguaje:	EN
Publicado:	Society for Promotion of Horticulture - Indian Institute of Horticultural Research 2009
Materias:	acclimatization chrysanthemum dendranthema grandiflora hardening micropropagation Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/b33625f226f849b8b6c76a2901ba586d
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spelling	oai:doaj.org-article:b33625f226f849b8b6c76a2901ba586d2021-12-02T12:10:31ZAcclimatization and Field Evaluation of Micropropagated Plants of Chrysanthemum Cv.'Arka Swarna'0973-354X2582-4899https://doaj.org/article/b33625f226f849b8b6c76a2901ba586d2009-06-01T00:00:00Zhttps://jhs.iihr.res.in/index.php/jhs/article/view/552https://doaj.org/toc/0973-354Xhttps://doaj.org/toc/2582-4899Chrysanthemum cv.'Arka Swarna' was micropropagated using shoot-tip and nodal microcuttings on MS medium containing 3% sucrose and 0.25% Phytagel® in the absence of externally supplied plant growth regulators, yielding 90-100% rooted plantlets, or in medium containing 1 μM benzyladenine or kinetin yielding 20-32% plantlets within 2-4 weeks of subculture. The stocks were acclimatized employing sachet technique wherein the rooted plantlets (2.5-4 cm) were planted in polythene bags of 5"×9" filled to one-third height with planting mixture. The closed bags with 1-5 plants were incubated under conditions similar to the in vitro stocks. The plantlets recorded 90-100% establishment within 4 weeks. The ex vitro established plants were evaluated in the field a month later by direct planting , or after one month in a field nursery-bed, along with conventional suckers. While field establishment (80-95%) was not significantly influenced by the treatments, micropropagated plants put through the nursery appeared to be the best among the three treatments in vegetative growth, floral characteristics and flower yield, demonstrating advantage of micropropagation over conventional propagation for shy-suckering chrysanthemums.Bindu PanickerPious ThomasT JanakiramSociety for Promotion of Horticulture - Indian Institute of Horticultural Researcharticleacclimatizationchrysanthemumdendranthema grandiflorahardeningmicropropagationPlant cultureSB1-1110ENJournal of Horticultural Sciences, Vol 4, Iss 1, Pp 32-35 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	acclimatization chrysanthemum dendranthema grandiflora hardening micropropagation Plant culture SB1-1110
spellingShingle	acclimatization chrysanthemum dendranthema grandiflora hardening micropropagation Plant culture SB1-1110 Bindu Panicker Pious Thomas T Janakiram Acclimatization and Field Evaluation of Micropropagated Plants of Chrysanthemum Cv.'Arka Swarna'
description	Chrysanthemum cv.'Arka Swarna' was micropropagated using shoot-tip and nodal microcuttings on MS medium containing 3% sucrose and 0.25% Phytagel® in the absence of externally supplied plant growth regulators, yielding 90-100% rooted plantlets, or in medium containing 1 μM benzyladenine or kinetin yielding 20-32% plantlets within 2-4 weeks of subculture. The stocks were acclimatized employing sachet technique wherein the rooted plantlets (2.5-4 cm) were planted in polythene bags of 5"×9" filled to one-third height with planting mixture. The closed bags with 1-5 plants were incubated under conditions similar to the in vitro stocks. The plantlets recorded 90-100% establishment within 4 weeks. The ex vitro established plants were evaluated in the field a month later by direct planting , or after one month in a field nursery-bed, along with conventional suckers. While field establishment (80-95%) was not significantly influenced by the treatments, micropropagated plants put through the nursery appeared to be the best among the three treatments in vegetative growth, floral characteristics and flower yield, demonstrating advantage of micropropagation over conventional propagation for shy-suckering chrysanthemums.
format	article
author	Bindu Panicker Pious Thomas T Janakiram
author_facet	Bindu Panicker Pious Thomas T Janakiram
author_sort	Bindu Panicker
title	Acclimatization and Field Evaluation of Micropropagated Plants of Chrysanthemum Cv.'Arka Swarna'
title_short	Acclimatization and Field Evaluation of Micropropagated Plants of Chrysanthemum Cv.'Arka Swarna'
title_full	Acclimatization and Field Evaluation of Micropropagated Plants of Chrysanthemum Cv.'Arka Swarna'
title_fullStr	Acclimatization and Field Evaluation of Micropropagated Plants of Chrysanthemum Cv.'Arka Swarna'
title_full_unstemmed	Acclimatization and Field Evaluation of Micropropagated Plants of Chrysanthemum Cv.'Arka Swarna'
title_sort	acclimatization and field evaluation of micropropagated plants of chrysanthemum cv.'arka swarna'
publisher	Society for Promotion of Horticulture - Indian Institute of Horticultural Research
publishDate	2009
url	https://doaj.org/article/b33625f226f849b8b6c76a2901ba586d
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Acclimatization and Field Evaluation of Micropropagated Plants of Chrysanthemum Cv.'Arka Swarna'

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