Multifunctional targeting micelle nanocarriers with both imaging and therapeutic potential for bladder cancer

Tzu-yin Lin,1 Hongyong Zhang,1 Juntao Luo,2,5 Yuanpei Li,2 Tingjuan Gao,3 Primo N Lara Jr,1,4,6 Ralph de Vere White,4 Kit S Lam,1,2 Chong-Xian Pan,1,3,61Division of Hematology and Oncology, Department of Internal Medicine, 2Department of Biochemistry and Molecular Medicine, 3NSF Center for Biophoton...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Lin TY, Zhang H, Luo J, Li Y, Gao T, Lara Jr PN, de Vere White R, Lam KS, Pan CX
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/b33b651757b049e7a5d42ff0db51455d
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:b33b651757b049e7a5d42ff0db51455d
record_format	dspace
spelling	oai:doaj.org-article:b33b651757b049e7a5d42ff0db51455d2021-12-02T07:56:17ZMultifunctional targeting micelle nanocarriers with both imaging and therapeutic potential for bladder cancer1176-91141178-2013https://doaj.org/article/b33b651757b049e7a5d42ff0db51455d2012-06-01T00:00:00Zhttp://www.dovepress.com/multifunctional-targeting-micelle-nanocarriers-with-both-imaging-and-t-a10050https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Tzu-yin Lin,1 Hongyong Zhang,1 Juntao Luo,2,5 Yuanpei Li,2 Tingjuan Gao,3 Primo N Lara Jr,1,4,6 Ralph de Vere White,4 Kit S Lam,1,2 Chong-Xian Pan,1,3,61Division of Hematology and Oncology, Department of Internal Medicine, 2Department of Biochemistry and Molecular Medicine, 3NSF Center for Biophotonics Science and Technology, School of Medicine, 4Department of Urology, University of California-Davis, Sacramento, CA, 5Department of Pharmacology, SUNY Upstate Medical University, Syracuse, NY, 6VA Northern California Health Care System, Mather, CA, USABackground: We previously developed a bladder cancer-specific ligand (PLZ4) that can specifically bind to both human and dog bladder cancer cells in vitro and in vivo. We have also developed a micelle nanocarrier drug-delivery system. Here, we assessed whether the targeting micelles decorated with PLZ4 on the surface could specifically target dog bladder cancer cells.Materials and methods: Micelle-building monomers (ie, telodendrimers) were synthesized through conjugation of polyethylene glycol with a cholic acid cluster at one end and PLZ4 at the other, which then self-assembled in an aqueous solution to form micelles. Dog bladder cancer cell lines were used for in vitro and in vivo drug delivery studies.Results: Compared to nontargeting micelles, targeting PLZ4 micelles (23.2 &plusmn; 8.1 nm in diameter) loaded with the imaging agent DiD and the chemotherapeutic drug paclitaxel or daunorubicin were more efficient in targeted drug delivery and more effective in cell killing in vitro. PLZ4 facilitated the uptake of micelles together with the cargo load into the target cells. We also developed an orthotopic invasive dog bladder cancer xenograft model in mice. In vivo studies with this model showed the targeting micelles were more efficient in targeted drug delivery than the free dye (14.3&times;; P &lt; 0.01) and nontargeting micelles (1.5&times;; P &lt; 0.05).Conclusion: Targeting micelles decorated with PLZ4 can selectively target dog bladder cancer cells and potentially be developed as imaging and therapeutic agents in a clinical setting. Preclinical studies of targeting micelles can be performed in dogs with spontaneous bladder cancer before proceeding with studies using human patients.Keywords: bladder urothelial carcinoma, nanoparticle, bladder cancer-specific peptide, targeted therapy, diagnostic imagingLin TYZhang HLuo JLi YGao TLara Jr PNde Vere White RLam KSPan CXDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 2793-2804 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Lin TY Zhang H Luo J Li Y Gao T Lara Jr PN de Vere White R Lam KS Pan CX Multifunctional targeting micelle nanocarriers with both imaging and therapeutic potential for bladder cancer
description	Tzu-yin Lin,1 Hongyong Zhang,1 Juntao Luo,2,5 Yuanpei Li,2 Tingjuan Gao,3 Primo N Lara Jr,1,4,6 Ralph de Vere White,4 Kit S Lam,1,2 Chong-Xian Pan,1,3,61Division of Hematology and Oncology, Department of Internal Medicine, 2Department of Biochemistry and Molecular Medicine, 3NSF Center for Biophotonics Science and Technology, School of Medicine, 4Department of Urology, University of California-Davis, Sacramento, CA, 5Department of Pharmacology, SUNY Upstate Medical University, Syracuse, NY, 6VA Northern California Health Care System, Mather, CA, USABackground: We previously developed a bladder cancer-specific ligand (PLZ4) that can specifically bind to both human and dog bladder cancer cells in vitro and in vivo. We have also developed a micelle nanocarrier drug-delivery system. Here, we assessed whether the targeting micelles decorated with PLZ4 on the surface could specifically target dog bladder cancer cells.Materials and methods: Micelle-building monomers (ie, telodendrimers) were synthesized through conjugation of polyethylene glycol with a cholic acid cluster at one end and PLZ4 at the other, which then self-assembled in an aqueous solution to form micelles. Dog bladder cancer cell lines were used for in vitro and in vivo drug delivery studies.Results: Compared to nontargeting micelles, targeting PLZ4 micelles (23.2 &plusmn; 8.1 nm in diameter) loaded with the imaging agent DiD and the chemotherapeutic drug paclitaxel or daunorubicin were more efficient in targeted drug delivery and more effective in cell killing in vitro. PLZ4 facilitated the uptake of micelles together with the cargo load into the target cells. We also developed an orthotopic invasive dog bladder cancer xenograft model in mice. In vivo studies with this model showed the targeting micelles were more efficient in targeted drug delivery than the free dye (14.3&times;; P &lt; 0.01) and nontargeting micelles (1.5&times;; P &lt; 0.05).Conclusion: Targeting micelles decorated with PLZ4 can selectively target dog bladder cancer cells and potentially be developed as imaging and therapeutic agents in a clinical setting. Preclinical studies of targeting micelles can be performed in dogs with spontaneous bladder cancer before proceeding with studies using human patients.Keywords: bladder urothelial carcinoma, nanoparticle, bladder cancer-specific peptide, targeted therapy, diagnostic imaging
format	article
author	Lin TY Zhang H Luo J Li Y Gao T Lara Jr PN de Vere White R Lam KS Pan CX
author_facet	Lin TY Zhang H Luo J Li Y Gao T Lara Jr PN de Vere White R Lam KS Pan CX
author_sort	Lin TY
title	Multifunctional targeting micelle nanocarriers with both imaging and therapeutic potential for bladder cancer
title_short	Multifunctional targeting micelle nanocarriers with both imaging and therapeutic potential for bladder cancer
title_full	Multifunctional targeting micelle nanocarriers with both imaging and therapeutic potential for bladder cancer
title_fullStr	Multifunctional targeting micelle nanocarriers with both imaging and therapeutic potential for bladder cancer
title_full_unstemmed	Multifunctional targeting micelle nanocarriers with both imaging and therapeutic potential for bladder cancer
title_sort	multifunctional targeting micelle nanocarriers with both imaging and therapeutic potential for bladder cancer
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/b33b651757b049e7a5d42ff0db51455d
work_keys_str_mv	AT linty multifunctionaltargetingmicellenanocarrierswithbothimagingandtherapeuticpotentialforbladdercancer AT zhangh multifunctionaltargetingmicellenanocarrierswithbothimagingandtherapeuticpotentialforbladdercancer AT luoj multifunctionaltargetingmicellenanocarrierswithbothimagingandtherapeuticpotentialforbladdercancer AT liy multifunctionaltargetingmicellenanocarrierswithbothimagingandtherapeuticpotentialforbladdercancer AT gaot multifunctionaltargetingmicellenanocarrierswithbothimagingandtherapeuticpotentialforbladdercancer AT larajrpn multifunctionaltargetingmicellenanocarrierswithbothimagingandtherapeuticpotentialforbladdercancer AT deverewhiter multifunctionaltargetingmicellenanocarrierswithbothimagingandtherapeuticpotentialforbladdercancer AT lamks multifunctionaltargetingmicellenanocarrierswithbothimagingandtherapeuticpotentialforbladdercancer AT pancx multifunctionaltargetingmicellenanocarrierswithbothimagingandtherapeuticpotentialforbladdercancer
_version_	1718399121775132672

Multifunctional targeting micelle nanocarriers with both imaging and therapeutic potential for bladder cancer

Ejemplares similares