Magnesium Sensing Regulates Intestinal Colonization of Enterohemorrhagic <named-content content-type="genus-species">Escherichia coli</named-content> O157:H7

ABSTRACT The large intestinal pathogen enterohemorrhagic Escherichia coli (EHEC) O157:H7 detects host cues to regulate virulence gene expression during colonization and infection. However, virulence regulatory mechanisms of EHEC O157:H7 in the human large intestine are not fully understood. Herein,...

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Autores principales:	Yutao Liu, Runhua Han, Junyue Wang, Pan Yang, Fang Wang, Bin Yang
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2020
Materias:	bacterial adherence magnesium locus of enterocyte effacement (LEE) virulence Z4267 gene regulation Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/b34329fc437e471797607ec7699c12ce
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spelling	oai:doaj.org-article:b34329fc437e471797607ec7699c12ce2021-11-15T15:55:43ZMagnesium Sensing Regulates Intestinal Colonization of Enterohemorrhagic <named-content content-type="genus-species">Escherichia coli</named-content> O157:H710.1128/mBio.02470-202150-7511https://doaj.org/article/b34329fc437e471797607ec7699c12ce2020-12-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.02470-20https://doaj.org/toc/2150-7511ABSTRACT The large intestinal pathogen enterohemorrhagic Escherichia coli (EHEC) O157:H7 detects host cues to regulate virulence gene expression during colonization and infection. However, virulence regulatory mechanisms of EHEC O157:H7 in the human large intestine are not fully understood. Herein, we identified a virulence-regulating pathway where the PhoQ/PhoP two-component regulatory system senses low magnesium levels and signals to the O island 119-encoded Z4267 (LmiA; low magnesium-induced regulator A), directly activating loci of enterocyte effacement genes to promote EHEC O157:H7 adherence in the large intestine. Disruption of this pathway significantly decreased EHEC O157:H7 adherence in the mouse intestinal tract. Moreover, feeding mice a magnesium-rich diet significantly reduced EHEC O157:H7 adherence in vivo. This LmiA-mediated virulence regulatory pathway is also conserved among several EHEC and enteropathogenic E. coli serotypes; therefore, our findings support the use of magnesium as a dietary supplement and provide greater insights into the dietary cues that can prevent enteric infections. IMPORTANCE Sensing specific gut metabolites is an important strategy for inducing crucial virulence programs by enterohemorrhagic Escherichia coli (EHEC) O157:H7 during colonization and infection. Here, we identified a virulence-regulating pathway wherein the PhoQ/PhoP two-component regulatory system signals to the O island 119-encoded low magnesium-induced regulator A (LmiA), which, in turn, activates locus of enterocyte effacement (LEE) genes to promote EHEC O157:H7 adherence in the low-magnesium conditions of the large intestine. This regulatory pathway is widely present in a range of EHEC and enteropathogenic E. coli (EPEC) serotypes. Disruption of this pathway significantly decreased EHEC O157:H7 adherence in the mouse intestinal tract. Moreover, mice fed a magnesium-rich diet showed significantly reduced EHEC O157:H7 adherence in vivo, indicating that magnesium may help in preventing EHEC and EPEC infection in humans.Yutao LiuRunhua HanJunyue WangPan YangFang WangBin YangAmerican Society for Microbiologyarticlebacterial adherencemagnesiumlocus of enterocyte effacement (LEE)virulenceZ4267gene regulationMicrobiologyQR1-502ENmBio, Vol 11, Iss 6 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	bacterial adherence magnesium locus of enterocyte effacement (LEE) virulence Z4267 gene regulation Microbiology QR1-502
spellingShingle	bacterial adherence magnesium locus of enterocyte effacement (LEE) virulence Z4267 gene regulation Microbiology QR1-502 Yutao Liu Runhua Han Junyue Wang Pan Yang Fang Wang Bin Yang Magnesium Sensing Regulates Intestinal Colonization of Enterohemorrhagic <named-content content-type="genus-species">Escherichia coli</named-content> O157:H7
description	ABSTRACT The large intestinal pathogen enterohemorrhagic Escherichia coli (EHEC) O157:H7 detects host cues to regulate virulence gene expression during colonization and infection. However, virulence regulatory mechanisms of EHEC O157:H7 in the human large intestine are not fully understood. Herein, we identified a virulence-regulating pathway where the PhoQ/PhoP two-component regulatory system senses low magnesium levels and signals to the O island 119-encoded Z4267 (LmiA; low magnesium-induced regulator A), directly activating loci of enterocyte effacement genes to promote EHEC O157:H7 adherence in the large intestine. Disruption of this pathway significantly decreased EHEC O157:H7 adherence in the mouse intestinal tract. Moreover, feeding mice a magnesium-rich diet significantly reduced EHEC O157:H7 adherence in vivo. This LmiA-mediated virulence regulatory pathway is also conserved among several EHEC and enteropathogenic E. coli serotypes; therefore, our findings support the use of magnesium as a dietary supplement and provide greater insights into the dietary cues that can prevent enteric infections. IMPORTANCE Sensing specific gut metabolites is an important strategy for inducing crucial virulence programs by enterohemorrhagic Escherichia coli (EHEC) O157:H7 during colonization and infection. Here, we identified a virulence-regulating pathway wherein the PhoQ/PhoP two-component regulatory system signals to the O island 119-encoded low magnesium-induced regulator A (LmiA), which, in turn, activates locus of enterocyte effacement (LEE) genes to promote EHEC O157:H7 adherence in the low-magnesium conditions of the large intestine. This regulatory pathway is widely present in a range of EHEC and enteropathogenic E. coli (EPEC) serotypes. Disruption of this pathway significantly decreased EHEC O157:H7 adherence in the mouse intestinal tract. Moreover, mice fed a magnesium-rich diet showed significantly reduced EHEC O157:H7 adherence in vivo, indicating that magnesium may help in preventing EHEC and EPEC infection in humans.
format	article
author	Yutao Liu Runhua Han Junyue Wang Pan Yang Fang Wang Bin Yang
author_facet	Yutao Liu Runhua Han Junyue Wang Pan Yang Fang Wang Bin Yang
author_sort	Yutao Liu
title	Magnesium Sensing Regulates Intestinal Colonization of Enterohemorrhagic <named-content content-type="genus-species">Escherichia coli</named-content> O157:H7
title_short	Magnesium Sensing Regulates Intestinal Colonization of Enterohemorrhagic <named-content content-type="genus-species">Escherichia coli</named-content> O157:H7
title_full	Magnesium Sensing Regulates Intestinal Colonization of Enterohemorrhagic <named-content content-type="genus-species">Escherichia coli</named-content> O157:H7
title_fullStr	Magnesium Sensing Regulates Intestinal Colonization of Enterohemorrhagic <named-content content-type="genus-species">Escherichia coli</named-content> O157:H7
title_full_unstemmed	Magnesium Sensing Regulates Intestinal Colonization of Enterohemorrhagic <named-content content-type="genus-species">Escherichia coli</named-content> O157:H7
title_sort	magnesium sensing regulates intestinal colonization of enterohemorrhagic <named-content content-type="genus-species">escherichia coli</named-content> o157:h7
publisher	American Society for Microbiology
publishDate	2020
url	https://doaj.org/article/b34329fc437e471797607ec7699c12ce
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Magnesium Sensing Regulates Intestinal Colonization of Enterohemorrhagic <named-content content-type="genus-species">Escherichia coli</named-content> O157:H7

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