Pooled CRISPRi screening of the cyanobacterium Synechocystis sp PCC 6803 for enhanced industrial phenotypes

Developing cyanobacteria as CO2-neutral cell factories relies on the knowledge of the regulation mechanisms for growth and metabolism. Here, the authors develop an inducible CRISPRi gene repression library in Synechocystis sp. PCC 6803 and screens genes potentially affecting growth and L-lactate tol...

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Autores principales:	Lun Yao, Kiyan Shabestary, Sara M. Björk, Johannes Asplund-Samuelsson, Haakan N. Joensson, Michael Jahn, Elton P. Hudson
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/b347e4caadcb4489961c56c34a9c1a76
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spelling	oai:doaj.org-article:b347e4caadcb4489961c56c34a9c1a762021-12-02T17:33:14ZPooled CRISPRi screening of the cyanobacterium Synechocystis sp PCC 6803 for enhanced industrial phenotypes10.1038/s41467-020-15491-72041-1723https://doaj.org/article/b347e4caadcb4489961c56c34a9c1a762020-04-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-15491-7https://doaj.org/toc/2041-1723Developing cyanobacteria as CO2-neutral cell factories relies on the knowledge of the regulation mechanisms for growth and metabolism. Here, the authors develop an inducible CRISPRi gene repression library in Synechocystis sp. PCC 6803 and screens genes potentially affecting growth and L-lactate tolerance and production.Lun YaoKiyan ShabestarySara M. BjörkJohannes Asplund-SamuelssonHaakan N. JoenssonMichael JahnElton P. HudsonNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-13 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Lun Yao Kiyan Shabestary Sara M. Björk Johannes Asplund-Samuelsson Haakan N. Joensson Michael Jahn Elton P. Hudson Pooled CRISPRi screening of the cyanobacterium Synechocystis sp PCC 6803 for enhanced industrial phenotypes
description	Developing cyanobacteria as CO2-neutral cell factories relies on the knowledge of the regulation mechanisms for growth and metabolism. Here, the authors develop an inducible CRISPRi gene repression library in Synechocystis sp. PCC 6803 and screens genes potentially affecting growth and L-lactate tolerance and production.
format	article
author	Lun Yao Kiyan Shabestary Sara M. Björk Johannes Asplund-Samuelsson Haakan N. Joensson Michael Jahn Elton P. Hudson
author_facet	Lun Yao Kiyan Shabestary Sara M. Björk Johannes Asplund-Samuelsson Haakan N. Joensson Michael Jahn Elton P. Hudson
author_sort	Lun Yao
title	Pooled CRISPRi screening of the cyanobacterium Synechocystis sp PCC 6803 for enhanced industrial phenotypes
title_short	Pooled CRISPRi screening of the cyanobacterium Synechocystis sp PCC 6803 for enhanced industrial phenotypes
title_full	Pooled CRISPRi screening of the cyanobacterium Synechocystis sp PCC 6803 for enhanced industrial phenotypes
title_fullStr	Pooled CRISPRi screening of the cyanobacterium Synechocystis sp PCC 6803 for enhanced industrial phenotypes
title_full_unstemmed	Pooled CRISPRi screening of the cyanobacterium Synechocystis sp PCC 6803 for enhanced industrial phenotypes
title_sort	pooled crispri screening of the cyanobacterium synechocystis sp pcc 6803 for enhanced industrial phenotypes
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/b347e4caadcb4489961c56c34a9c1a76
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_version_	1718380020900036608

Pooled CRISPRi screening of the cyanobacterium Synechocystis sp PCC 6803 for enhanced industrial phenotypes

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