Novel Chitosan-Silica Hybrid Hydrogels for Cell Encapsulation and Drug Delivery

Hydrogels constructed from naturally derived polymers provide an aqueous environment that encourages cell growth, however, mechanical properties are poor and degradation can be difficult to predict. Whilst, synthetic hydrogels exhibit some improved mechanical properties, these materials lack biochem...

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Autores principales:	Soher N. Jayash, Paul R. Cooper, Richard M. Shelton, Sarah A. Kuehne, Gowsihan Poologasundarampillai
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	chitosan thiolated chitosan organic-inorganic hybrid hydrogel sol-gel process cell encapsulation drug delivery Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/b34821a2af5941c5b45b4ddf6a9d0d5a
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spelling	oai:doaj.org-article:b34821a2af5941c5b45b4ddf6a9d0d5a2021-11-25T17:54:51ZNovel Chitosan-Silica Hybrid Hydrogels for Cell Encapsulation and Drug Delivery10.3390/ijms2222122671422-00671661-6596https://doaj.org/article/b34821a2af5941c5b45b4ddf6a9d0d5a2021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/22/12267https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Hydrogels constructed from naturally derived polymers provide an aqueous environment that encourages cell growth, however, mechanical properties are poor and degradation can be difficult to predict. Whilst, synthetic hydrogels exhibit some improved mechanical properties, these materials lack biochemical cues for cells growing and have limited biodegradation. To produce hydrogels that support 3D cell cultures to form tissue mimics, materials must exhibit appropriate biological and mechanical properties. In this study, novel organic-inorganic hybrid hydrogels based on chitosan and silica were prepared using the sol-gel technique. The chemical, physical and biological properties of the hydrogels were assessed. Statistical analysis was performed using One-Way ANOVAs and independent-sample <i>t</i>-tests. Fourier transform infrared spectroscopy showed characteristic absorption bands including amide II, Si-O and Si-O-Si confirming formation of hybrid networks. Oscillatory rheometry was used to characterise the sol to gel transition and viscoelastic behaviour of hydrogels. Furthermore, in vitro degradation revealed both chitosan and silica were released over 21 days. The hydrogels exhibited high loading efficiency as total protein loading was released in a week. There were significant differences between TC<sub>2</sub>G and C<sub>2</sub>G at all-time points (<i>p</i> < 0.05). The viability of osteoblasts seeded on, and encapsulated within, the hydrogels was >70% over 168 h culture and antimicrobial activity was demonstrated against <i>Pseudomonas aeruginosa</i> and <i>Enterococcus faecalis</i>. The hydrogels developed here offer alternatives for biopolymer hydrogels for biomedical use, including for application in drug/cell delivery and for bone tissue engineering.Soher N. JayashPaul R. CooperRichard M. SheltonSarah A. KuehneGowsihan PoologasundarampillaiMDPI AGarticlechitosanthiolated chitosanorganic-inorganic hybrid hydrogelsol-gel processcell encapsulationdrug deliveryBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12267, p 12267 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	chitosan thiolated chitosan organic-inorganic hybrid hydrogel sol-gel process cell encapsulation drug delivery Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	chitosan thiolated chitosan organic-inorganic hybrid hydrogel sol-gel process cell encapsulation drug delivery Biology (General) QH301-705.5 Chemistry QD1-999 Soher N. Jayash Paul R. Cooper Richard M. Shelton Sarah A. Kuehne Gowsihan Poologasundarampillai Novel Chitosan-Silica Hybrid Hydrogels for Cell Encapsulation and Drug Delivery
description	Hydrogels constructed from naturally derived polymers provide an aqueous environment that encourages cell growth, however, mechanical properties are poor and degradation can be difficult to predict. Whilst, synthetic hydrogels exhibit some improved mechanical properties, these materials lack biochemical cues for cells growing and have limited biodegradation. To produce hydrogels that support 3D cell cultures to form tissue mimics, materials must exhibit appropriate biological and mechanical properties. In this study, novel organic-inorganic hybrid hydrogels based on chitosan and silica were prepared using the sol-gel technique. The chemical, physical and biological properties of the hydrogels were assessed. Statistical analysis was performed using One-Way ANOVAs and independent-sample <i>t</i>-tests. Fourier transform infrared spectroscopy showed characteristic absorption bands including amide II, Si-O and Si-O-Si confirming formation of hybrid networks. Oscillatory rheometry was used to characterise the sol to gel transition and viscoelastic behaviour of hydrogels. Furthermore, in vitro degradation revealed both chitosan and silica were released over 21 days. The hydrogels exhibited high loading efficiency as total protein loading was released in a week. There were significant differences between TC<sub>2</sub>G and C<sub>2</sub>G at all-time points (<i>p</i> < 0.05). The viability of osteoblasts seeded on, and encapsulated within, the hydrogels was >70% over 168 h culture and antimicrobial activity was demonstrated against <i>Pseudomonas aeruginosa</i> and <i>Enterococcus faecalis</i>. The hydrogels developed here offer alternatives for biopolymer hydrogels for biomedical use, including for application in drug/cell delivery and for bone tissue engineering.
format	article
author	Soher N. Jayash Paul R. Cooper Richard M. Shelton Sarah A. Kuehne Gowsihan Poologasundarampillai
author_facet	Soher N. Jayash Paul R. Cooper Richard M. Shelton Sarah A. Kuehne Gowsihan Poologasundarampillai
author_sort	Soher N. Jayash
title	Novel Chitosan-Silica Hybrid Hydrogels for Cell Encapsulation and Drug Delivery
title_short	Novel Chitosan-Silica Hybrid Hydrogels for Cell Encapsulation and Drug Delivery
title_full	Novel Chitosan-Silica Hybrid Hydrogels for Cell Encapsulation and Drug Delivery
title_fullStr	Novel Chitosan-Silica Hybrid Hydrogels for Cell Encapsulation and Drug Delivery
title_full_unstemmed	Novel Chitosan-Silica Hybrid Hydrogels for Cell Encapsulation and Drug Delivery
title_sort	novel chitosan-silica hybrid hydrogels for cell encapsulation and drug delivery
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/b34821a2af5941c5b45b4ddf6a9d0d5a
work_keys_str_mv	AT sohernjayash novelchitosansilicahybridhydrogelsforcellencapsulationanddrugdelivery AT paulrcooper novelchitosansilicahybridhydrogelsforcellencapsulationanddrugdelivery AT richardmshelton novelchitosansilicahybridhydrogelsforcellencapsulationanddrugdelivery AT sarahakuehne novelchitosansilicahybridhydrogelsforcellencapsulationanddrugdelivery AT gowsihanpoologasundarampillai novelchitosansilicahybridhydrogelsforcellencapsulationanddrugdelivery
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Novel Chitosan-Silica Hybrid Hydrogels for Cell Encapsulation and Drug Delivery

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