Screening and molecular identification of lipolytic bacteria from spent bleaching earth

Abstract. Soleha S, Retnaningrum E. 2020. Screening and molecular identification of lipolytic bacteria from spent bleaching earth. Biodiversitas 21: 4155-4161. Lipolytic bacteria can produce nanobioremediation materials that encourage researchers to explore the diversity of these bacteria. Lipolytic...

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Autores principales:	SITI SOLEHA, Endah Retnaningrum
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2020
Materias:	16s rrna, lipolytic index, moraxella sp, nanobioremediation, phylogenetic tree Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/b34cacfda281453e8674d8fdfa91bb15
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spelling	oai:doaj.org-article:b34cacfda281453e8674d8fdfa91bb152021-11-22T00:41:43ZScreening and molecular identification of lipolytic bacteria from spent bleaching earth1412-033X2085-472210.13057/biodiv/d210929https://doaj.org/article/b34cacfda281453e8674d8fdfa91bb152020-08-01T00:00:00Zhttps://smujo.id/biodiv/article/view/6073https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Soleha S, Retnaningrum E. 2020. Screening and molecular identification of lipolytic bacteria from spent bleaching earth. Biodiversitas 21: 4155-4161. Lipolytic bacteria can produce nanobioremediation materials that encourage researchers to explore the diversity of these bacteria. Lipolytic bacteria were isolated from spent bleaching earth (SBE). Bacteria isolates were screened for their lipolytic index and crude lipase activity. Strain with the highest lipolytic index and crude lipase activity were identified using the 16S rRNA gene as genetic markers. The maximum likelihood method in MEGA6 software has been used to analyze the relationship of the isolate. The results showed that strain SBE01 had the highest lipolytic index and crude lipase activity of 8.8 ± 0.7 mm and 5.7 U/mL, respectively. Based on 16S rRNA gene analysis, the isolate was identified as Moraxella sp. Phylogenetic tree was shown that SBE01 isolate was closely related to Moraxella osloensis. Moraxella sp. has the potential to be used as a bioremediation agent from oil-contaminated environment.SITI SOLEHAEndah RetnaningrumMBI & UNS Soloarticle16s rrna, lipolytic index, moraxella sp, nanobioremediation, phylogenetic treeBiology (General)QH301-705.5ENBiodiversitas, Vol 21, Iss 9 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	16s rrna, lipolytic index, moraxella sp, nanobioremediation, phylogenetic tree Biology (General) QH301-705.5
spellingShingle	16s rrna, lipolytic index, moraxella sp, nanobioremediation, phylogenetic tree Biology (General) QH301-705.5 SITI SOLEHA Endah Retnaningrum Screening and molecular identification of lipolytic bacteria from spent bleaching earth
description	Abstract. Soleha S, Retnaningrum E. 2020. Screening and molecular identification of lipolytic bacteria from spent bleaching earth. Biodiversitas 21: 4155-4161. Lipolytic bacteria can produce nanobioremediation materials that encourage researchers to explore the diversity of these bacteria. Lipolytic bacteria were isolated from spent bleaching earth (SBE). Bacteria isolates were screened for their lipolytic index and crude lipase activity. Strain with the highest lipolytic index and crude lipase activity were identified using the 16S rRNA gene as genetic markers. The maximum likelihood method in MEGA6 software has been used to analyze the relationship of the isolate. The results showed that strain SBE01 had the highest lipolytic index and crude lipase activity of 8.8 ± 0.7 mm and 5.7 U/mL, respectively. Based on 16S rRNA gene analysis, the isolate was identified as Moraxella sp. Phylogenetic tree was shown that SBE01 isolate was closely related to Moraxella osloensis. Moraxella sp. has the potential to be used as a bioremediation agent from oil-contaminated environment.
format	article
author	SITI SOLEHA Endah Retnaningrum
author_facet	SITI SOLEHA Endah Retnaningrum
author_sort	SITI SOLEHA
title	Screening and molecular identification of lipolytic bacteria from spent bleaching earth
title_short	Screening and molecular identification of lipolytic bacteria from spent bleaching earth
title_full	Screening and molecular identification of lipolytic bacteria from spent bleaching earth
title_fullStr	Screening and molecular identification of lipolytic bacteria from spent bleaching earth
title_full_unstemmed	Screening and molecular identification of lipolytic bacteria from spent bleaching earth
title_sort	screening and molecular identification of lipolytic bacteria from spent bleaching earth
publisher	MBI & UNS Solo
publishDate	2020
url	https://doaj.org/article/b34cacfda281453e8674d8fdfa91bb15
work_keys_str_mv	AT sitisoleha screeningandmolecularidentificationoflipolyticbacteriafromspentbleachingearth AT endahretnaningrum screeningandmolecularidentificationoflipolyticbacteriafromspentbleachingearth
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Screening and molecular identification of lipolytic bacteria from spent bleaching earth

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