Development of a DNA Bar-coding Project as a Biology Laboratory Module

This article is intended for faculty who are looking for new techniques for teaching a genetics or molecular ecology lab. We have used the bar-coding protocol for both a non-majors watershed ecology lab and a majors-specific genetics lab with equal success. The exercise involves extracting mitochond...

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Autores principales:	David Dunbar, Caroline Nielsen
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2010
Materias:	Special aspects of education LC8-6691 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/b34cd258ee0d47a8a8c46b8e3d0b5ac7
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spelling	oai:doaj.org-article:b34cd258ee0d47a8a8c46b8e3d0b5ac72021-11-15T15:04:11ZDevelopment of a DNA Bar-coding Project as a Biology Laboratory Module10.1128/jmbe.v11i2.1791935-78851935-7877https://doaj.org/article/b34cd258ee0d47a8a8c46b8e3d0b5ac72010-01-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/jmbe.v11i2.179https://doaj.org/toc/1935-7877https://doaj.org/toc/1935-7885This article is intended for faculty who are looking for new techniques for teaching a genetics or molecular ecology lab. We have used the bar-coding protocol for both a non-majors watershed ecology lab and a majors-specific genetics lab with equal success. The exercise involves extracting mitochondrial DNA from animal tissue, amplifying a portion of the mitochondrial DNA by the polymerase chain reaction (PCR), and sequencing the amplified DNA to determine the animal to the species level. Logistically, time spent on the DNA bar-coding procedure could be as short as 2–3 weeks or last an entire semester, depending on course outcomes and time availability.David DunbarCaroline NielsenAmerican Society for MicrobiologyarticleSpecial aspects of educationLC8-6691Biology (General)QH301-705.5ENJournal of Microbiology & Biology Education, Vol 11, Iss 2, Pp 160-161 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Special aspects of education LC8-6691 Biology (General) QH301-705.5
spellingShingle	Special aspects of education LC8-6691 Biology (General) QH301-705.5 David Dunbar Caroline Nielsen Development of a DNA Bar-coding Project as a Biology Laboratory Module
description	This article is intended for faculty who are looking for new techniques for teaching a genetics or molecular ecology lab. We have used the bar-coding protocol for both a non-majors watershed ecology lab and a majors-specific genetics lab with equal success. The exercise involves extracting mitochondrial DNA from animal tissue, amplifying a portion of the mitochondrial DNA by the polymerase chain reaction (PCR), and sequencing the amplified DNA to determine the animal to the species level. Logistically, time spent on the DNA bar-coding procedure could be as short as 2–3 weeks or last an entire semester, depending on course outcomes and time availability.
format	article
author	David Dunbar Caroline Nielsen
author_facet	David Dunbar Caroline Nielsen
author_sort	David Dunbar
title	Development of a DNA Bar-coding Project as a Biology Laboratory Module
title_short	Development of a DNA Bar-coding Project as a Biology Laboratory Module
title_full	Development of a DNA Bar-coding Project as a Biology Laboratory Module
title_fullStr	Development of a DNA Bar-coding Project as a Biology Laboratory Module
title_full_unstemmed	Development of a DNA Bar-coding Project as a Biology Laboratory Module
title_sort	development of a dna bar-coding project as a biology laboratory module
publisher	American Society for Microbiology
publishDate	2010
url	https://doaj.org/article/b34cd258ee0d47a8a8c46b8e3d0b5ac7
work_keys_str_mv	AT daviddunbar developmentofadnabarcodingprojectasabiologylaboratorymodule AT carolinenielsen developmentofadnabarcodingprojectasabiologylaboratorymodule
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Development of a DNA Bar-coding Project as a Biology Laboratory Module

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