The Arabidopsis thaliana homeobox gene ATHB12 is involved in symptom development caused by geminivirus infection.

<h4>Background</h4>Geminiviruses are single-stranded DNA viruses that infect a number of monocotyledonous and dicotyledonous plants. Arabidopsis is susceptible to infection with the Curtovirus, Beet severe curly top virus (BSCTV). Infection of Arabidopsis with BSCTV causes severe symptom...

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Autores principales:	Jungan Park, Hyun-Ju Lee, Choong-Ill Cheon, Sung-Han Kim, Yoon-Sun Hur, Chung-Kyun Auh, Kyung-Hwan Im, Dae-Jin Yun, Sukchan Lee, Keith R Davis
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b34e78c67da94780a171e55ab25e2a20
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spelling	oai:doaj.org-article:b34e78c67da94780a171e55ab25e2a202021-11-18T06:53:45ZThe Arabidopsis thaliana homeobox gene ATHB12 is involved in symptom development caused by geminivirus infection.1932-620310.1371/journal.pone.0020054https://doaj.org/article/b34e78c67da94780a171e55ab25e2a202011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21625602/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Geminiviruses are single-stranded DNA viruses that infect a number of monocotyledonous and dicotyledonous plants. Arabidopsis is susceptible to infection with the Curtovirus, Beet severe curly top virus (BSCTV). Infection of Arabidopsis with BSCTV causes severe symptoms characterized by stunting, leaf curling, and the development of abnormal inflorescence and root structures. BSCTV-induced symptom development requires the virus-encoded C4 protein which is thought to interact with specific plant-host proteins and disrupt signaling pathways important for controlling cell division and development. Very little is known about the specific plant regulatory factors that participate in BSCTV-induced symptom development. This study was conducted to identify specific transcription factors that are induced by BSCTV infection.<h4>Methodology/principal findings</h4>Arabidopsis plants were inoculated with BSCTV and the induction of specific transcription factors was monitored using quantitative real-time polymerase chain reaction assays. We found that the ATHB12 and ATHB7 genes, members of the homeodomain-leucine zipper family of transcription factors previously shown to be induced by abscisic acid and water stress, are induced in symptomatic tissues of Arabidopsis inoculated with BSCTV. ATHB12 expression is correlated with an array of morphological abnormalities including leaf curling, stunting, and callus-like structures in infected Arabidopsis. Inoculation of plants with a BSCTV mutant with a defective c4 gene failed to induce ATHB12. Transgenic plants expressing the BSCTV C4 gene exhibited increased ATHB12 expression whereas BSCTV-infected ATHB12 knock-down plants developed milder symptoms and had lower ATHB12 expression compared to the wild-type plants. Reporter gene studies demonstrated that the ATHB12 promoter was responsive to BSCTV infection and the highest expression levels were observed in symptomatic tissues where cell cycle genes also were induced.<h4>Conclusions/significance</h4>These results suggest that ATHB7 and ATHB12 may play an important role in the activation of the abnormal cell division associated with symptom development during geminivirus infection.Jungan ParkHyun-Ju LeeChoong-Ill CheonSung-Han KimYoon-Sun HurChung-Kyun AuhKyung-Hwan ImDae-Jin YunSukchan LeeKeith R DavisPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 5, p e20054 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jungan Park Hyun-Ju Lee Choong-Ill Cheon Sung-Han Kim Yoon-Sun Hur Chung-Kyun Auh Kyung-Hwan Im Dae-Jin Yun Sukchan Lee Keith R Davis The Arabidopsis thaliana homeobox gene ATHB12 is involved in symptom development caused by geminivirus infection.
description	<h4>Background</h4>Geminiviruses are single-stranded DNA viruses that infect a number of monocotyledonous and dicotyledonous plants. Arabidopsis is susceptible to infection with the Curtovirus, Beet severe curly top virus (BSCTV). Infection of Arabidopsis with BSCTV causes severe symptoms characterized by stunting, leaf curling, and the development of abnormal inflorescence and root structures. BSCTV-induced symptom development requires the virus-encoded C4 protein which is thought to interact with specific plant-host proteins and disrupt signaling pathways important for controlling cell division and development. Very little is known about the specific plant regulatory factors that participate in BSCTV-induced symptom development. This study was conducted to identify specific transcription factors that are induced by BSCTV infection.<h4>Methodology/principal findings</h4>Arabidopsis plants were inoculated with BSCTV and the induction of specific transcription factors was monitored using quantitative real-time polymerase chain reaction assays. We found that the ATHB12 and ATHB7 genes, members of the homeodomain-leucine zipper family of transcription factors previously shown to be induced by abscisic acid and water stress, are induced in symptomatic tissues of Arabidopsis inoculated with BSCTV. ATHB12 expression is correlated with an array of morphological abnormalities including leaf curling, stunting, and callus-like structures in infected Arabidopsis. Inoculation of plants with a BSCTV mutant with a defective c4 gene failed to induce ATHB12. Transgenic plants expressing the BSCTV C4 gene exhibited increased ATHB12 expression whereas BSCTV-infected ATHB12 knock-down plants developed milder symptoms and had lower ATHB12 expression compared to the wild-type plants. Reporter gene studies demonstrated that the ATHB12 promoter was responsive to BSCTV infection and the highest expression levels were observed in symptomatic tissues where cell cycle genes also were induced.<h4>Conclusions/significance</h4>These results suggest that ATHB7 and ATHB12 may play an important role in the activation of the abnormal cell division associated with symptom development during geminivirus infection.
format	article
author	Jungan Park Hyun-Ju Lee Choong-Ill Cheon Sung-Han Kim Yoon-Sun Hur Chung-Kyun Auh Kyung-Hwan Im Dae-Jin Yun Sukchan Lee Keith R Davis
author_facet	Jungan Park Hyun-Ju Lee Choong-Ill Cheon Sung-Han Kim Yoon-Sun Hur Chung-Kyun Auh Kyung-Hwan Im Dae-Jin Yun Sukchan Lee Keith R Davis
author_sort	Jungan Park
title	The Arabidopsis thaliana homeobox gene ATHB12 is involved in symptom development caused by geminivirus infection.
title_short	The Arabidopsis thaliana homeobox gene ATHB12 is involved in symptom development caused by geminivirus infection.
title_full	The Arabidopsis thaliana homeobox gene ATHB12 is involved in symptom development caused by geminivirus infection.
title_fullStr	The Arabidopsis thaliana homeobox gene ATHB12 is involved in symptom development caused by geminivirus infection.
title_full_unstemmed	The Arabidopsis thaliana homeobox gene ATHB12 is involved in symptom development caused by geminivirus infection.
title_sort	arabidopsis thaliana homeobox gene athb12 is involved in symptom development caused by geminivirus infection.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/b34e78c67da94780a171e55ab25e2a20
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The Arabidopsis thaliana homeobox gene ATHB12 is involved in symptom development caused by geminivirus infection.

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