Unexpected functional implication of a stable succinimide in the structural stability of Methanocaldococcus jannaschii glutaminase

Succinimide is a post-translational modification susceptible to rapid hydrolysis and generally associated with protein destabilisation. Here, the authors use mass spectroscopy to identify a stable succinimide intermediate that is responsible for the high thermostability of a thermophilic enzyme.

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Autores principales:	Sanjeev Kumar, Sunita Prakash, Kallol Gupta, Aparna Dongre, Padmanabhan Balaram, Hemalatha Balaram
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2016
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/b34f767175e64052bff780cd4687ca4d
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spelling	oai:doaj.org-article:b34f767175e64052bff780cd4687ca4d2021-12-02T16:50:56ZUnexpected functional implication of a stable succinimide in the structural stability of Methanocaldococcus jannaschii glutaminase10.1038/ncomms127982041-1723https://doaj.org/article/b34f767175e64052bff780cd4687ca4d2016-09-01T00:00:00Zhttps://doi.org/10.1038/ncomms12798https://doaj.org/toc/2041-1723Succinimide is a post-translational modification susceptible to rapid hydrolysis and generally associated with protein destabilisation. Here, the authors use mass spectroscopy to identify a stable succinimide intermediate that is responsible for the high thermostability of a thermophilic enzyme.Sanjeev KumarSunita PrakashKallol GuptaAparna DongrePadmanabhan BalaramHemalatha BalaramNature PortfolioarticleScienceQENNature Communications, Vol 7, Iss 1, Pp 1-14 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Sanjeev Kumar Sunita Prakash Kallol Gupta Aparna Dongre Padmanabhan Balaram Hemalatha Balaram Unexpected functional implication of a stable succinimide in the structural stability of Methanocaldococcus jannaschii glutaminase
description	Succinimide is a post-translational modification susceptible to rapid hydrolysis and generally associated with protein destabilisation. Here, the authors use mass spectroscopy to identify a stable succinimide intermediate that is responsible for the high thermostability of a thermophilic enzyme.
format	article
author	Sanjeev Kumar Sunita Prakash Kallol Gupta Aparna Dongre Padmanabhan Balaram Hemalatha Balaram
author_facet	Sanjeev Kumar Sunita Prakash Kallol Gupta Aparna Dongre Padmanabhan Balaram Hemalatha Balaram
author_sort	Sanjeev Kumar
title	Unexpected functional implication of a stable succinimide in the structural stability of Methanocaldococcus jannaschii glutaminase
title_short	Unexpected functional implication of a stable succinimide in the structural stability of Methanocaldococcus jannaschii glutaminase
title_full	Unexpected functional implication of a stable succinimide in the structural stability of Methanocaldococcus jannaschii glutaminase
title_fullStr	Unexpected functional implication of a stable succinimide in the structural stability of Methanocaldococcus jannaschii glutaminase
title_full_unstemmed	Unexpected functional implication of a stable succinimide in the structural stability of Methanocaldococcus jannaschii glutaminase
title_sort	unexpected functional implication of a stable succinimide in the structural stability of methanocaldococcus jannaschii glutaminase
publisher	Nature Portfolio
publishDate	2016
url	https://doaj.org/article/b34f767175e64052bff780cd4687ca4d
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Unexpected functional implication of a stable succinimide in the structural stability of Methanocaldococcus jannaschii glutaminase

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