Evaluation of protein kinase D auto-phosphorylation as biomarker for NLRP3 inflammasome activation

<h4>Background</h4> The NLRP3 inflammasome is a critical component of sterile inflammation, which is involved in many diseases. However, there is currently no known proximal biomarker for measuring NLRP3 activation in pathological conditions. Protein kinase D (PKD) has emerged as an impo...

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Autores principales:	Diane Heiser, Joëlle Rubert, Adeline Unterreiner, Claudine Maurer, Marion Kamke, Ursula Bodendorf, Christopher J. Farady, Ben Roediger, Frédéric Bornancin
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b34f9244fab24b9282945afea3cea706
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spelling	oai:doaj.org-article:b34f9244fab24b9282945afea3cea7062021-11-25T06:11:00ZEvaluation of protein kinase D auto-phosphorylation as biomarker for NLRP3 inflammasome activation1932-6203https://doaj.org/article/b34f9244fab24b9282945afea3cea7062021-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8589197/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4> The NLRP3 inflammasome is a critical component of sterile inflammation, which is involved in many diseases. However, there is currently no known proximal biomarker for measuring NLRP3 activation in pathological conditions. Protein kinase D (PKD) has emerged as an important NLRP3 kinase that catalyzes the release of a phosphorylated NLRP3 species that is competent for inflammasome complex assembly. <h4>Methods</h4> To explore the potential for PKD activation to serve as a selective biomarker of the NLRP3 pathway, we tested various stimulatory conditions in THP-1 and U937 cell lines, probing the inflammasome space beyond NLRP3. We analyzed the correlation between PKD activation (monitored by its auto-phosphorylation) and functional inflammasome readouts. <h4>Results</h4> PKD activation/auto-phosphorylation always preceded cleavage of caspase-1 and gasdermin D, and treatment with the PKD inhibitor CRT0066101 could block NLRP3 inflammasome assembly and interleukin-1β production. Conversely, blocking NLRP3 either genetically or using the MCC950 inhibitor prevented PKD auto-phosphorylation, indicating a bidirectional functional crosstalk between NLRP3 and PKD. Further assessments of the pyrin and NLRC4 pathways, however, revealed that PKD auto-phosphorylation can be triggered by a broad range of stimuli unrelated to NLRP3 inflammasome assembly. <h4>Conclusion</h4> Although PKD and NLRP3 become functionally interconnected during NLRP3 activation, the promiscuous reactivity of PKD challenges its potential use for tracing the NLRP3 inflammasome pathway.Diane HeiserJoëlle RubertAdeline UnterreinerClaudine MaurerMarion KamkeUrsula BodendorfChristopher J. FaradyBen RoedigerFrédéric BornancinPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Diane Heiser Joëlle Rubert Adeline Unterreiner Claudine Maurer Marion Kamke Ursula Bodendorf Christopher J. Farady Ben Roediger Frédéric Bornancin Evaluation of protein kinase D auto-phosphorylation as biomarker for NLRP3 inflammasome activation
description	<h4>Background</h4> The NLRP3 inflammasome is a critical component of sterile inflammation, which is involved in many diseases. However, there is currently no known proximal biomarker for measuring NLRP3 activation in pathological conditions. Protein kinase D (PKD) has emerged as an important NLRP3 kinase that catalyzes the release of a phosphorylated NLRP3 species that is competent for inflammasome complex assembly. <h4>Methods</h4> To explore the potential for PKD activation to serve as a selective biomarker of the NLRP3 pathway, we tested various stimulatory conditions in THP-1 and U937 cell lines, probing the inflammasome space beyond NLRP3. We analyzed the correlation between PKD activation (monitored by its auto-phosphorylation) and functional inflammasome readouts. <h4>Results</h4> PKD activation/auto-phosphorylation always preceded cleavage of caspase-1 and gasdermin D, and treatment with the PKD inhibitor CRT0066101 could block NLRP3 inflammasome assembly and interleukin-1β production. Conversely, blocking NLRP3 either genetically or using the MCC950 inhibitor prevented PKD auto-phosphorylation, indicating a bidirectional functional crosstalk between NLRP3 and PKD. Further assessments of the pyrin and NLRC4 pathways, however, revealed that PKD auto-phosphorylation can be triggered by a broad range of stimuli unrelated to NLRP3 inflammasome assembly. <h4>Conclusion</h4> Although PKD and NLRP3 become functionally interconnected during NLRP3 activation, the promiscuous reactivity of PKD challenges its potential use for tracing the NLRP3 inflammasome pathway.
format	article
author	Diane Heiser Joëlle Rubert Adeline Unterreiner Claudine Maurer Marion Kamke Ursula Bodendorf Christopher J. Farady Ben Roediger Frédéric Bornancin
author_facet	Diane Heiser Joëlle Rubert Adeline Unterreiner Claudine Maurer Marion Kamke Ursula Bodendorf Christopher J. Farady Ben Roediger Frédéric Bornancin
author_sort	Diane Heiser
title	Evaluation of protein kinase D auto-phosphorylation as biomarker for NLRP3 inflammasome activation
title_short	Evaluation of protein kinase D auto-phosphorylation as biomarker for NLRP3 inflammasome activation
title_full	Evaluation of protein kinase D auto-phosphorylation as biomarker for NLRP3 inflammasome activation
title_fullStr	Evaluation of protein kinase D auto-phosphorylation as biomarker for NLRP3 inflammasome activation
title_full_unstemmed	Evaluation of protein kinase D auto-phosphorylation as biomarker for NLRP3 inflammasome activation
title_sort	evaluation of protein kinase d auto-phosphorylation as biomarker for nlrp3 inflammasome activation
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/b34f9244fab24b9282945afea3cea706
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Evaluation of protein kinase D auto-phosphorylation as biomarker for NLRP3 inflammasome activation

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