Melanocytes in black-boned chicken have immune contribution under infectious bursal disease virus infection

ABSTRACT: In black-boned chicken, melanocytes are widely distributed in their inner organs. However, the roles of these cells are not fully elucidated. In this study, we used 3-wk-old female Silky Fowl to investigate the functions of melanocytes under infection with infectious bursal disease virus (...

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Autores principales:	Deping Han, Yurong Tai, Guoying Hua, Xue Yang, Jianfei Chen, Junying Li, Xuemei Deng
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	melanocyte avian mucosal immunity infectious bursa disease virus Animal culture SF1-1100
Acceso en línea:	https://doaj.org/article/b358e5d67daa47c0bfe546bd385f75ed
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spelling	oai:doaj.org-article:b358e5d67daa47c0bfe546bd385f75ed2021-11-24T04:22:37ZMelanocytes in black-boned chicken have immune contribution under infectious bursal disease virus infection0032-579110.1016/j.psj.2021.101498https://doaj.org/article/b358e5d67daa47c0bfe546bd385f75ed2021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S0032579121005204https://doaj.org/toc/0032-5791ABSTRACT: In black-boned chicken, melanocytes are widely distributed in their inner organs. However, the roles of these cells are not fully elucidated. In this study, we used 3-wk-old female Silky Fowl to investigate the functions of melanocytes under infection with infectious bursal disease virus (IBDV). We found the melanocytes in the bursa of Fabricius involved in IBDV infection shown as abundant melanin were transported into the nodule and lamina propria where obvious apoptotic cells and higher expression of BAX were detected. Genes related to the toll-like receptor (TLR) signaling pathway were highly detected by quantitative PCR, including TLR1, TLR3, TLR4, TLR15, myeloid differential protein-88, interferon-α, and interferon-β. We then isolated and infected primary melanocytes with IBDV in vitro and found that higher expressions of immune genes were detected at 24 and 48 h after infection; the upregulated innate and adaptive immune genes were involved in the pathogenesis of IBDV infection, including TLR3, TLR7, interleukin 15 (IL15), IL18, IL1rap, CD7, BG2, ERAP1, and SLA2. These changes in gene expression were highly associated with microtubule-based movement, antigen processing and presentation, defense against viruses, and innate immune responses. Our results indicated that the widely distributed melanocytes in Silky Fowl could migrate to play important innate immune roles during virus infection.Deping HanYurong TaiGuoying HuaXue YangJianfei ChenJunying LiXuemei DengElsevierarticlemelanocyteavianmucosal immunityinfectious bursa disease virusAnimal cultureSF1-1100ENPoultry Science, Vol 100, Iss 12, Pp 101498- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	melanocyte avian mucosal immunity infectious bursa disease virus Animal culture SF1-1100
spellingShingle	melanocyte avian mucosal immunity infectious bursa disease virus Animal culture SF1-1100 Deping Han Yurong Tai Guoying Hua Xue Yang Jianfei Chen Junying Li Xuemei Deng Melanocytes in black-boned chicken have immune contribution under infectious bursal disease virus infection
description	ABSTRACT: In black-boned chicken, melanocytes are widely distributed in their inner organs. However, the roles of these cells are not fully elucidated. In this study, we used 3-wk-old female Silky Fowl to investigate the functions of melanocytes under infection with infectious bursal disease virus (IBDV). We found the melanocytes in the bursa of Fabricius involved in IBDV infection shown as abundant melanin were transported into the nodule and lamina propria where obvious apoptotic cells and higher expression of BAX were detected. Genes related to the toll-like receptor (TLR) signaling pathway were highly detected by quantitative PCR, including TLR1, TLR3, TLR4, TLR15, myeloid differential protein-88, interferon-α, and interferon-β. We then isolated and infected primary melanocytes with IBDV in vitro and found that higher expressions of immune genes were detected at 24 and 48 h after infection; the upregulated innate and adaptive immune genes were involved in the pathogenesis of IBDV infection, including TLR3, TLR7, interleukin 15 (IL15), IL18, IL1rap, CD7, BG2, ERAP1, and SLA2. These changes in gene expression were highly associated with microtubule-based movement, antigen processing and presentation, defense against viruses, and innate immune responses. Our results indicated that the widely distributed melanocytes in Silky Fowl could migrate to play important innate immune roles during virus infection.
format	article
author	Deping Han Yurong Tai Guoying Hua Xue Yang Jianfei Chen Junying Li Xuemei Deng
author_facet	Deping Han Yurong Tai Guoying Hua Xue Yang Jianfei Chen Junying Li Xuemei Deng
author_sort	Deping Han
title	Melanocytes in black-boned chicken have immune contribution under infectious bursal disease virus infection
title_short	Melanocytes in black-boned chicken have immune contribution under infectious bursal disease virus infection
title_full	Melanocytes in black-boned chicken have immune contribution under infectious bursal disease virus infection
title_fullStr	Melanocytes in black-boned chicken have immune contribution under infectious bursal disease virus infection
title_full_unstemmed	Melanocytes in black-boned chicken have immune contribution under infectious bursal disease virus infection
title_sort	melanocytes in black-boned chicken have immune contribution under infectious bursal disease virus infection
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/b358e5d67daa47c0bfe546bd385f75ed
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Melanocytes in black-boned chicken have immune contribution under infectious bursal disease virus infection

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