Wafer-Scale Patterning of Protein Templates for Hydrogel Fabrication

Human-induced pluripotent stem cell-derived cardiomyocytes are a potentially unlimited cell source and promising patient-specific in vitro model of cardiac diseases. Yet, these cells are limited by immaturity and population heterogeneity. Current in vitro studies aiming at better understanding of th...

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Autores principales:	Anna A. Kim, Erica A. Castillo, Kerry V. Lane, Gabriela V. Torres, Orlando Chirikian, Robin E. Wilson, Sydney A. Lance, Gaspard Pardon, Beth L. Pruitt
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	microfabrication lift-off protein patterning hydrogels single-cell cardiomyocytes single-cell analysis Mechanical engineering and machinery TJ1-1570
Acceso en línea:	https://doaj.org/article/b35a717fbaa54a8eb0387e860132b500
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spelling	oai:doaj.org-article:b35a717fbaa54a8eb0387e860132b5002021-11-25T18:23:35ZWafer-Scale Patterning of Protein Templates for Hydrogel Fabrication10.3390/mi121113862072-666Xhttps://doaj.org/article/b35a717fbaa54a8eb0387e860132b5002021-11-01T00:00:00Zhttps://www.mdpi.com/2072-666X/12/11/1386https://doaj.org/toc/2072-666XHuman-induced pluripotent stem cell-derived cardiomyocytes are a potentially unlimited cell source and promising patient-specific in vitro model of cardiac diseases. Yet, these cells are limited by immaturity and population heterogeneity. Current in vitro studies aiming at better understanding of the mechanical and chemical cues in the microenvironment that drive cellular maturation involve deformable materials and precise manipulation of the microenvironment with, for example, micropatterns. Such microenvironment manipulation most often involves microfabrication protocols which are time-consuming, require cleanroom facilities and photolithography expertise. Here, we present a method to increase the scale of the fabrication pipeline, thereby enabling large-batch generation of shelf-stable microenvironment protein templates on glass chips. This decreases fabrication time and allows for more flexibility in the subsequent steps, for example, in tuning the material properties and the selection of extracellular matrix or cell proteins. Further, the fabrication of deformable hydrogels has been optimized for compatibility with these templates, in addition to the templates being able to be used to acquire protein patterns directly on the glass chips. With our approach, we have successfully controlled the shapes of cardiomyocytes seeded on Matrigel-patterned hydrogels.Anna A. KimErica A. CastilloKerry V. LaneGabriela V. TorresOrlando ChirikianRobin E. WilsonSydney A. LanceGaspard PardonBeth L. PruittMDPI AGarticlemicrofabricationlift-off protein patterninghydrogelssingle-cell cardiomyocytessingle-cell analysisMechanical engineering and machineryTJ1-1570ENMicromachines, Vol 12, Iss 1386, p 1386 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	microfabrication lift-off protein patterning hydrogels single-cell cardiomyocytes single-cell analysis Mechanical engineering and machinery TJ1-1570
spellingShingle	microfabrication lift-off protein patterning hydrogels single-cell cardiomyocytes single-cell analysis Mechanical engineering and machinery TJ1-1570 Anna A. Kim Erica A. Castillo Kerry V. Lane Gabriela V. Torres Orlando Chirikian Robin E. Wilson Sydney A. Lance Gaspard Pardon Beth L. Pruitt Wafer-Scale Patterning of Protein Templates for Hydrogel Fabrication
description	Human-induced pluripotent stem cell-derived cardiomyocytes are a potentially unlimited cell source and promising patient-specific in vitro model of cardiac diseases. Yet, these cells are limited by immaturity and population heterogeneity. Current in vitro studies aiming at better understanding of the mechanical and chemical cues in the microenvironment that drive cellular maturation involve deformable materials and precise manipulation of the microenvironment with, for example, micropatterns. Such microenvironment manipulation most often involves microfabrication protocols which are time-consuming, require cleanroom facilities and photolithography expertise. Here, we present a method to increase the scale of the fabrication pipeline, thereby enabling large-batch generation of shelf-stable microenvironment protein templates on glass chips. This decreases fabrication time and allows for more flexibility in the subsequent steps, for example, in tuning the material properties and the selection of extracellular matrix or cell proteins. Further, the fabrication of deformable hydrogels has been optimized for compatibility with these templates, in addition to the templates being able to be used to acquire protein patterns directly on the glass chips. With our approach, we have successfully controlled the shapes of cardiomyocytes seeded on Matrigel-patterned hydrogels.
format	article
author	Anna A. Kim Erica A. Castillo Kerry V. Lane Gabriela V. Torres Orlando Chirikian Robin E. Wilson Sydney A. Lance Gaspard Pardon Beth L. Pruitt
author_facet	Anna A. Kim Erica A. Castillo Kerry V. Lane Gabriela V. Torres Orlando Chirikian Robin E. Wilson Sydney A. Lance Gaspard Pardon Beth L. Pruitt
author_sort	Anna A. Kim
title	Wafer-Scale Patterning of Protein Templates for Hydrogel Fabrication
title_short	Wafer-Scale Patterning of Protein Templates for Hydrogel Fabrication
title_full	Wafer-Scale Patterning of Protein Templates for Hydrogel Fabrication
title_fullStr	Wafer-Scale Patterning of Protein Templates for Hydrogel Fabrication
title_full_unstemmed	Wafer-Scale Patterning of Protein Templates for Hydrogel Fabrication
title_sort	wafer-scale patterning of protein templates for hydrogel fabrication
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/b35a717fbaa54a8eb0387e860132b500
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Wafer-Scale Patterning of Protein Templates for Hydrogel Fabrication

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