Electrochemical impedimetric biosensor based on a nanostructured polycarbonate substrate

Yu-Shan Chen1, Chia-Che Wu1, Jaw-Ji Tsai2, Gou-Jen Wang1,31Department of Mechanical Engineering, National Chung-Hsing University, 2Department of Medical Education and Research, Taichung Veterans General Hospital, 3Graduate Institute of Biomedical Engineering, National Chung-Hsing University, Taichun...

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Autores principales:	Chen YS, Wu CC, Tsai JJ, Wang GJ
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/b366e4e9c72d47ef8345a024739a79bd
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spelling	oai:doaj.org-article:b366e4e9c72d47ef8345a024739a79bd2021-12-02T04:20:59ZElectrochemical impedimetric biosensor based on a nanostructured polycarbonate substrate1176-91141178-2013https://doaj.org/article/b366e4e9c72d47ef8345a024739a79bd2012-01-01T00:00:00Zhttp://www.dovepress.com/electrochemical-impedimetric-biosensor-based-on-a-nanostructured-polyc-a9010https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Yu-Shan Chen1, Chia-Che Wu1, Jaw-Ji Tsai2, Gou-Jen Wang1,31Department of Mechanical Engineering, National Chung-Hsing University, 2Department of Medical Education and Research, Taichung Veterans General Hospital, 3Graduate Institute of Biomedical Engineering, National Chung-Hsing University, Taichung, TaiwanAbstract: This study integrates the techniques of nanoelectroforming, hot-embossing, and electrochemical deposition to develop a disposable, low-cost, and high sensitivity nanostructure biosensor. A modified anodic aluminum oxide barrier-layer surface was used as the template for thin nickel film deposition. After etching the anodic aluminum oxide template off, a three-dimensional mold of the concave nanostructure array was created. The fabricated three-dimensional nickel mold was further used for replica molding of a nanostructure polycarbonate substrate by hot-embossing. A thin gold film was then sputtered onto the polycarbonate substrate to form the electrode, followed by deposition of an orderly and uniform gold nanoparticle layer on the three-dimensional gold electrode using electrochemical deposition. Finally, silver nanoparticles were deposited on the uniformly deposited gold nanoparticles to enhance the conductivity of the sensor. Electrochemical impedance spectroscopy analysis was then used to detect the concentration of the target element. The sensitivity of the proposed scheme on the detection of the dust mite antigen, Der p2, reached 0.1 pg/mL.Keywords: nanoelectroforming, nanostructure polycarbonate substrate, gold nanoparticles, silver nanoparticles, electrochemical impedance spectroscopyChen YSWu CCTsai JJWang GJDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 133-140 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Chen YS Wu CC Tsai JJ Wang GJ Electrochemical impedimetric biosensor based on a nanostructured polycarbonate substrate
description	Yu-Shan Chen1, Chia-Che Wu1, Jaw-Ji Tsai2, Gou-Jen Wang1,31Department of Mechanical Engineering, National Chung-Hsing University, 2Department of Medical Education and Research, Taichung Veterans General Hospital, 3Graduate Institute of Biomedical Engineering, National Chung-Hsing University, Taichung, TaiwanAbstract: This study integrates the techniques of nanoelectroforming, hot-embossing, and electrochemical deposition to develop a disposable, low-cost, and high sensitivity nanostructure biosensor. A modified anodic aluminum oxide barrier-layer surface was used as the template for thin nickel film deposition. After etching the anodic aluminum oxide template off, a three-dimensional mold of the concave nanostructure array was created. The fabricated three-dimensional nickel mold was further used for replica molding of a nanostructure polycarbonate substrate by hot-embossing. A thin gold film was then sputtered onto the polycarbonate substrate to form the electrode, followed by deposition of an orderly and uniform gold nanoparticle layer on the three-dimensional gold electrode using electrochemical deposition. Finally, silver nanoparticles were deposited on the uniformly deposited gold nanoparticles to enhance the conductivity of the sensor. Electrochemical impedance spectroscopy analysis was then used to detect the concentration of the target element. The sensitivity of the proposed scheme on the detection of the dust mite antigen, Der p2, reached 0.1 pg/mL.Keywords: nanoelectroforming, nanostructure polycarbonate substrate, gold nanoparticles, silver nanoparticles, electrochemical impedance spectroscopy
format	article
author	Chen YS Wu CC Tsai JJ Wang GJ
author_facet	Chen YS Wu CC Tsai JJ Wang GJ
author_sort	Chen YS
title	Electrochemical impedimetric biosensor based on a nanostructured polycarbonate substrate
title_short	Electrochemical impedimetric biosensor based on a nanostructured polycarbonate substrate
title_full	Electrochemical impedimetric biosensor based on a nanostructured polycarbonate substrate
title_fullStr	Electrochemical impedimetric biosensor based on a nanostructured polycarbonate substrate
title_full_unstemmed	Electrochemical impedimetric biosensor based on a nanostructured polycarbonate substrate
title_sort	electrochemical impedimetric biosensor based on a nanostructured polycarbonate substrate
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/b366e4e9c72d47ef8345a024739a79bd
work_keys_str_mv	AT chenys electrochemicalimpedimetricbiosensorbasedonananostructuredpolycarbonatesubstrate AT wucc electrochemicalimpedimetricbiosensorbasedonananostructuredpolycarbonatesubstrate AT tsaijj electrochemicalimpedimetricbiosensorbasedonananostructuredpolycarbonatesubstrate AT wanggj electrochemicalimpedimetricbiosensorbasedonananostructuredpolycarbonatesubstrate
_version_	1718401330103451648

Electrochemical impedimetric biosensor based on a nanostructured polycarbonate substrate

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