A portable epigenetic switch for bistable gene expression in bacteria

Abstract We describe a portable epigenetic switch based on opvAB, a Salmonella enterica operon that undergoes bistable expression under DNA methylation control. A DNA fragment containing the opvAB promoter and the opvAB upstream regulatory region confers bistability to heterologous genes, yielding O...

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Autores principales:	David R. Olivenza, Hervé Nicoloff, María Antonia Sánchez-Romero, Ignacio Cota, Dan I. Andersson, Josep Casadesús
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b36d5c100fd74377906c0304d92d7e3f
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spelling	oai:doaj.org-article:b36d5c100fd74377906c0304d92d7e3f2021-12-02T15:09:00ZA portable epigenetic switch for bistable gene expression in bacteria10.1038/s41598-019-47650-22045-2322https://doaj.org/article/b36d5c100fd74377906c0304d92d7e3f2019-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-47650-2https://doaj.org/toc/2045-2322Abstract We describe a portable epigenetic switch based on opvAB, a Salmonella enterica operon that undergoes bistable expression under DNA methylation control. A DNA fragment containing the opvAB promoter and the opvAB upstream regulatory region confers bistability to heterologous genes, yielding OFF and ON subpopulations. Bistable expression under opvAB control is reproducible in Escherichia coli, showing that the opvAB switch can be functional in a heterologous host. Subpopulations of different sizes can be produced at will using engineered opvAB variants. Controlled formation of antibiotic-resistant and antibiotic-susceptible subpopulations may allow use of the opvAB switch in the study of bacterial heteroresistance to antibiotics.David R. OlivenzaHervé NicoloffMaría Antonia Sánchez-RomeroIgnacio CotaDan I. AnderssonJosep CasadesúsNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-9 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q David R. Olivenza Hervé Nicoloff María Antonia Sánchez-Romero Ignacio Cota Dan I. Andersson Josep Casadesús A portable epigenetic switch for bistable gene expression in bacteria
description	Abstract We describe a portable epigenetic switch based on opvAB, a Salmonella enterica operon that undergoes bistable expression under DNA methylation control. A DNA fragment containing the opvAB promoter and the opvAB upstream regulatory region confers bistability to heterologous genes, yielding OFF and ON subpopulations. Bistable expression under opvAB control is reproducible in Escherichia coli, showing that the opvAB switch can be functional in a heterologous host. Subpopulations of different sizes can be produced at will using engineered opvAB variants. Controlled formation of antibiotic-resistant and antibiotic-susceptible subpopulations may allow use of the opvAB switch in the study of bacterial heteroresistance to antibiotics.
format	article
author	David R. Olivenza Hervé Nicoloff María Antonia Sánchez-Romero Ignacio Cota Dan I. Andersson Josep Casadesús
author_facet	David R. Olivenza Hervé Nicoloff María Antonia Sánchez-Romero Ignacio Cota Dan I. Andersson Josep Casadesús
author_sort	David R. Olivenza
title	A portable epigenetic switch for bistable gene expression in bacteria
title_short	A portable epigenetic switch for bistable gene expression in bacteria
title_full	A portable epigenetic switch for bistable gene expression in bacteria
title_fullStr	A portable epigenetic switch for bistable gene expression in bacteria
title_full_unstemmed	A portable epigenetic switch for bistable gene expression in bacteria
title_sort	portable epigenetic switch for bistable gene expression in bacteria
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/b36d5c100fd74377906c0304d92d7e3f
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A portable epigenetic switch for bistable gene expression in bacteria

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