Molecular Characterization of Phytophthora nicotianae Associated with Diseases of Horticultural Crops by RFLP of PCR Internal Transcribed Spacer Region of Ribosomal DNA and AFLP Fingerprints

Molecular characterization of Phytophthora isolates from tobacco, tomato, carnation, gerbera, crossandra and periwinkle was carried out using internal transcribed spacer(ITS) regions of rDNA gene repeat and amplified fragment length polymorphism (AFLP). All the isolates of Phytophthora were identica...

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Autor principal:	P Chowdappa
Formato:	article
Lenguaje:	EN
Publicado:	Society for Promotion of Horticulture - Indian Institute of Horticultural Research 2007
Materias:	phytophthora nicotianae aflp its-rflp Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/b36e2638127142b486627653dd56b2b5
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spelling	oai:doaj.org-article:b36e2638127142b486627653dd56b2b52021-12-02T12:52:11ZMolecular Characterization of Phytophthora nicotianae Associated with Diseases of Horticultural Crops by RFLP of PCR Internal Transcribed Spacer Region of Ribosomal DNA and AFLP Fingerprints0973-354X2582-4899https://doaj.org/article/b36e2638127142b486627653dd56b2b52007-06-01T00:00:00Zhttps://jhs.iihr.res.in/index.php/jhs/article/view/640https://doaj.org/toc/0973-354Xhttps://doaj.org/toc/2582-4899Molecular characterization of Phytophthora isolates from tobacco, tomato, carnation, gerbera, crossandra and periwinkle was carried out using internal transcribed spacer(ITS) regions of rDNA gene repeat and amplified fragment length polymorphism (AFLP). All the isolates of Phytophthora were identical in morphology and ITSRFLP patterns, indicating P. nicotianae and P. parasitica are synonymous. However, based on AFLP, four sub groups were evident within population of P. nicotianae (P. parasitica): Group I, includes isolates from tomato, tobacco and periwinkle. Isolates from carnation represents Group II. Isolates from gerbera falls under group III. The crossandra isolates formed a Group IV. Thus, present study showed the existence of four molecular sub groups within population of P. nicotianae (P. parasitica) in India.P ChowdappaSociety for Promotion of Horticulture - Indian Institute of Horticultural Researcharticlephytophthora nicotianaeaflpits-rflpPlant cultureSB1-1110ENJournal of Horticultural Sciences, Vol 2, Iss 1, Pp 58-62 (2007)
institution	DOAJ
collection	DOAJ
language	EN
topic	phytophthora nicotianae aflp its-rflp Plant culture SB1-1110
spellingShingle	phytophthora nicotianae aflp its-rflp Plant culture SB1-1110 P Chowdappa Molecular Characterization of Phytophthora nicotianae Associated with Diseases of Horticultural Crops by RFLP of PCR Internal Transcribed Spacer Region of Ribosomal DNA and AFLP Fingerprints
description	Molecular characterization of Phytophthora isolates from tobacco, tomato, carnation, gerbera, crossandra and periwinkle was carried out using internal transcribed spacer(ITS) regions of rDNA gene repeat and amplified fragment length polymorphism (AFLP). All the isolates of Phytophthora were identical in morphology and ITSRFLP patterns, indicating P. nicotianae and P. parasitica are synonymous. However, based on AFLP, four sub groups were evident within population of P. nicotianae (P. parasitica): Group I, includes isolates from tomato, tobacco and periwinkle. Isolates from carnation represents Group II. Isolates from gerbera falls under group III. The crossandra isolates formed a Group IV. Thus, present study showed the existence of four molecular sub groups within population of P. nicotianae (P. parasitica) in India.
format	article
author	P Chowdappa
author_facet	P Chowdappa
author_sort	P Chowdappa
title	Molecular Characterization of Phytophthora nicotianae Associated with Diseases of Horticultural Crops by RFLP of PCR Internal Transcribed Spacer Region of Ribosomal DNA and AFLP Fingerprints
title_short	Molecular Characterization of Phytophthora nicotianae Associated with Diseases of Horticultural Crops by RFLP of PCR Internal Transcribed Spacer Region of Ribosomal DNA and AFLP Fingerprints
title_full	Molecular Characterization of Phytophthora nicotianae Associated with Diseases of Horticultural Crops by RFLP of PCR Internal Transcribed Spacer Region of Ribosomal DNA and AFLP Fingerprints
title_fullStr	Molecular Characterization of Phytophthora nicotianae Associated with Diseases of Horticultural Crops by RFLP of PCR Internal Transcribed Spacer Region of Ribosomal DNA and AFLP Fingerprints
title_full_unstemmed	Molecular Characterization of Phytophthora nicotianae Associated with Diseases of Horticultural Crops by RFLP of PCR Internal Transcribed Spacer Region of Ribosomal DNA and AFLP Fingerprints
title_sort	molecular characterization of phytophthora nicotianae associated with diseases of horticultural crops by rflp of pcr internal transcribed spacer region of ribosomal dna and aflp fingerprints
publisher	Society for Promotion of Horticulture - Indian Institute of Horticultural Research
publishDate	2007
url	https://doaj.org/article/b36e2638127142b486627653dd56b2b5
work_keys_str_mv	AT pchowdappa molecularcharacterizationofphytophthoranicotianaeassociatedwithdiseasesofhorticulturalcropsbyrflpofpcrinternaltranscribedspacerregionofribosomaldnaandaflpfingerprints
_version_	1718393645358383104

Molecular Characterization of Phytophthora nicotianae Associated with Diseases of Horticultural Crops by RFLP of PCR Internal Transcribed Spacer Region of Ribosomal DNA and AFLP Fingerprints

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