Diversity oriented biosynthesis via accelerated evolution of modular gene clusters
Reengineering polyketide synthase encoding genes to produce analogues of natural products can be slow and low-yielding. Here the authors use accelerated evolution to recombine the gene cluster for rapid production of rapamycin-related products.
Guardado en:
| Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Nature Portfolio
2017
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/b36ef21246ab47bfb8cbd4e969caea7c |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:b36ef21246ab47bfb8cbd4e969caea7c |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:b36ef21246ab47bfb8cbd4e969caea7c2021-12-02T14:42:27ZDiversity oriented biosynthesis via accelerated evolution of modular gene clusters10.1038/s41467-017-01344-32041-1723https://doaj.org/article/b36ef21246ab47bfb8cbd4e969caea7c2017-10-01T00:00:00Zhttps://doi.org/10.1038/s41467-017-01344-3https://doaj.org/toc/2041-1723Reengineering polyketide synthase encoding genes to produce analogues of natural products can be slow and low-yielding. Here the authors use accelerated evolution to recombine the gene cluster for rapid production of rapamycin-related products.Aleksandra WlodekSteve G. KendrewNigel J. CoatesAdam HoldJoanna PogwizdSteven RudderLesley S. SheehanSarah J. HigginbothamAnna E. Stanley-SmithTony WarneckMohammad Nur-E-AlamMarkus RadzomChristine J. MartinLois OvervoordeMarkiyan SamborskyySilke AltDaniel HeineGuy T. CarterEdmund I. GrazianiFrank E. KoehnLeonard McDonaldAlexander AlanineRosa María Rodríguez SarmientoSuzan Keen ChaoHasane RatniLucinda StewardIsobel H. NorvilleMitali Sarkar-TysonSteven J. MossPeter F. LeadlayBarrie WilkinsonMatthew A. GregoryNature PortfolioarticleScienceQENNature Communications, Vol 8, Iss 1, Pp 1-10 (2017) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Science Q |
| spellingShingle |
Science Q Aleksandra Wlodek Steve G. Kendrew Nigel J. Coates Adam Hold Joanna Pogwizd Steven Rudder Lesley S. Sheehan Sarah J. Higginbotham Anna E. Stanley-Smith Tony Warneck Mohammad Nur-E-Alam Markus Radzom Christine J. Martin Lois Overvoorde Markiyan Samborskyy Silke Alt Daniel Heine Guy T. Carter Edmund I. Graziani Frank E. Koehn Leonard McDonald Alexander Alanine Rosa María Rodríguez Sarmiento Suzan Keen Chao Hasane Ratni Lucinda Steward Isobel H. Norville Mitali Sarkar-Tyson Steven J. Moss Peter F. Leadlay Barrie Wilkinson Matthew A. Gregory Diversity oriented biosynthesis via accelerated evolution of modular gene clusters |
| description |
Reengineering polyketide synthase encoding genes to produce analogues of natural products can be slow and low-yielding. Here the authors use accelerated evolution to recombine the gene cluster for rapid production of rapamycin-related products. |
| format |
article |
| author |
Aleksandra Wlodek Steve G. Kendrew Nigel J. Coates Adam Hold Joanna Pogwizd Steven Rudder Lesley S. Sheehan Sarah J. Higginbotham Anna E. Stanley-Smith Tony Warneck Mohammad Nur-E-Alam Markus Radzom Christine J. Martin Lois Overvoorde Markiyan Samborskyy Silke Alt Daniel Heine Guy T. Carter Edmund I. Graziani Frank E. Koehn Leonard McDonald Alexander Alanine Rosa María Rodríguez Sarmiento Suzan Keen Chao Hasane Ratni Lucinda Steward Isobel H. Norville Mitali Sarkar-Tyson Steven J. Moss Peter F. Leadlay Barrie Wilkinson Matthew A. Gregory |
| author_facet |
Aleksandra Wlodek Steve G. Kendrew Nigel J. Coates Adam Hold Joanna Pogwizd Steven Rudder Lesley S. Sheehan Sarah J. Higginbotham Anna E. Stanley-Smith Tony Warneck Mohammad Nur-E-Alam Markus Radzom Christine J. Martin Lois Overvoorde Markiyan Samborskyy Silke Alt Daniel Heine Guy T. Carter Edmund I. Graziani Frank E. Koehn Leonard McDonald Alexander Alanine Rosa María Rodríguez Sarmiento Suzan Keen Chao Hasane Ratni Lucinda Steward Isobel H. Norville Mitali Sarkar-Tyson Steven J. Moss Peter F. Leadlay Barrie Wilkinson Matthew A. Gregory |
| author_sort |
Aleksandra Wlodek |
| title |
Diversity oriented biosynthesis via accelerated evolution of modular gene clusters |
| title_short |
Diversity oriented biosynthesis via accelerated evolution of modular gene clusters |
| title_full |
Diversity oriented biosynthesis via accelerated evolution of modular gene clusters |
| title_fullStr |
Diversity oriented biosynthesis via accelerated evolution of modular gene clusters |
| title_full_unstemmed |
Diversity oriented biosynthesis via accelerated evolution of modular gene clusters |
| title_sort |
diversity oriented biosynthesis via accelerated evolution of modular gene clusters |
| publisher |
Nature Portfolio |
| publishDate |
2017 |
| url |
https://doaj.org/article/b36ef21246ab47bfb8cbd4e969caea7c |
| work_keys_str_mv |
AT aleksandrawlodek diversityorientedbiosynthesisviaacceleratedevolutionofmodulargeneclusters AT stevegkendrew diversityorientedbiosynthesisviaacceleratedevolutionofmodulargeneclusters AT nigeljcoates diversityorientedbiosynthesisviaacceleratedevolutionofmodulargeneclusters AT adamhold diversityorientedbiosynthesisviaacceleratedevolutionofmodulargeneclusters AT joannapogwizd diversityorientedbiosynthesisviaacceleratedevolutionofmodulargeneclusters AT stevenrudder diversityorientedbiosynthesisviaacceleratedevolutionofmodulargeneclusters AT lesleyssheehan diversityorientedbiosynthesisviaacceleratedevolutionofmodulargeneclusters AT sarahjhigginbotham diversityorientedbiosynthesisviaacceleratedevolutionofmodulargeneclusters AT annaestanleysmith diversityorientedbiosynthesisviaacceleratedevolutionofmodulargeneclusters AT tonywarneck diversityorientedbiosynthesisviaacceleratedevolutionofmodulargeneclusters AT mohammadnurealam diversityorientedbiosynthesisviaacceleratedevolutionofmodulargeneclusters AT markusradzom diversityorientedbiosynthesisviaacceleratedevolutionofmodulargeneclusters AT christinejmartin diversityorientedbiosynthesisviaacceleratedevolutionofmodulargeneclusters AT loisovervoorde diversityorientedbiosynthesisviaacceleratedevolutionofmodulargeneclusters AT markiyansamborskyy diversityorientedbiosynthesisviaacceleratedevolutionofmodulargeneclusters AT silkealt diversityorientedbiosynthesisviaacceleratedevolutionofmodulargeneclusters AT danielheine diversityorientedbiosynthesisviaacceleratedevolutionofmodulargeneclusters AT guytcarter diversityorientedbiosynthesisviaacceleratedevolutionofmodulargeneclusters AT edmundigraziani diversityorientedbiosynthesisviaacceleratedevolutionofmodulargeneclusters AT frankekoehn diversityorientedbiosynthesisviaacceleratedevolutionofmodulargeneclusters AT leonardmcdonald diversityorientedbiosynthesisviaacceleratedevolutionofmodulargeneclusters AT alexanderalanine diversityorientedbiosynthesisviaacceleratedevolutionofmodulargeneclusters AT rosamariarodriguezsarmiento diversityorientedbiosynthesisviaacceleratedevolutionofmodulargeneclusters AT suzankeenchao diversityorientedbiosynthesisviaacceleratedevolutionofmodulargeneclusters AT hasaneratni diversityorientedbiosynthesisviaacceleratedevolutionofmodulargeneclusters AT lucindasteward diversityorientedbiosynthesisviaacceleratedevolutionofmodulargeneclusters AT isobelhnorville diversityorientedbiosynthesisviaacceleratedevolutionofmodulargeneclusters AT mitalisarkartyson diversityorientedbiosynthesisviaacceleratedevolutionofmodulargeneclusters AT stevenjmoss diversityorientedbiosynthesisviaacceleratedevolutionofmodulargeneclusters AT peterfleadlay diversityorientedbiosynthesisviaacceleratedevolutionofmodulargeneclusters AT barriewilkinson diversityorientedbiosynthesisviaacceleratedevolutionofmodulargeneclusters AT matthewagregory diversityorientedbiosynthesisviaacceleratedevolutionofmodulargeneclusters |
| _version_ |
1718389711021539328 |