Characterization of <i>Escherichia coli</i> from Edible Insect Species: Detection of Shiga Toxin-Producing Isolate

Insects as novel foods are gaining popularity in Europe. Regulation (EU) 2015/2283 laid the framework for the application process to market food insects in member states, but potential hazards are still being evaluated. The aim of this study was to investigate samples of edible insect species for th...

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Autores principales:	Anja Müller, Diana Seinige, Nils T. Grabowski, Birte Ahlfeld, Min Yue, Corinna Kehrenberg
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	Shiga toxin <i>Escherichia coli</i> antimicrobial resistance edible insects Chemical technology TP1-1185
Acceso en línea:	https://doaj.org/article/b3701ebda792412f9f23dbf3a5e1da68
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spelling	oai:doaj.org-article:b3701ebda792412f9f23dbf3a5e1da682021-11-25T17:32:32ZCharacterization of <i>Escherichia coli</i> from Edible Insect Species: Detection of Shiga Toxin-Producing Isolate10.3390/foods101125522304-8158https://doaj.org/article/b3701ebda792412f9f23dbf3a5e1da682021-10-01T00:00:00Zhttps://www.mdpi.com/2304-8158/10/11/2552https://doaj.org/toc/2304-8158Insects as novel foods are gaining popularity in Europe. Regulation (EU) 2015/2283 laid the framework for the application process to market food insects in member states, but potential hazards are still being evaluated. The aim of this study was to investigate samples of edible insect species for the presence of antimicrobial-resistant and Shiga toxin-producing <i>Escherichia coli</i> (STEC). Twenty-one <i>E. coli</i> isolates, recovered from samples of five different edible insect species, were subjected to antimicrobial susceptibility testing, PCR-based phylotyping, and macrorestriction analysis. The presence of genes associated with antimicrobial resistance or virulence, including <i>stx1</i>, <i>stx2,</i> and <i>eae</i>, was investigated by PCR. All isolates were subjected to genome sequencing, multilocus sequence typing, and serotype prediction. The isolates belonged either to phylogenetic group A, comprising mostly commensal <i>E. coli</i>, or group B1. One O178:H7 isolate, recovered from a <i>Zophobas atratus</i> sample, was identified as a STEC. A single isolate was resistant to tetracyclines and carried the <i>tet</i>(B) gene. Overall, this study shows that STEC can be present in edible insects, representing a potential health hazard. In contrast, the low resistance rate among the isolates indicates a low risk for the transmission of antimicrobial-resistant <i>E. coli</i> to consumers.Anja MüllerDiana SeinigeNils T. GrabowskiBirte AhlfeldMin YueCorinna KehrenbergMDPI AGarticleShiga toxin<i>Escherichia coli</i>antimicrobial resistanceedible insectsChemical technologyTP1-1185ENFoods, Vol 10, Iss 2552, p 2552 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Shiga toxin <i>Escherichia coli</i> antimicrobial resistance edible insects Chemical technology TP1-1185
spellingShingle	Shiga toxin <i>Escherichia coli</i> antimicrobial resistance edible insects Chemical technology TP1-1185 Anja Müller Diana Seinige Nils T. Grabowski Birte Ahlfeld Min Yue Corinna Kehrenberg Characterization of <i>Escherichia coli</i> from Edible Insect Species: Detection of Shiga Toxin-Producing Isolate
description	Insects as novel foods are gaining popularity in Europe. Regulation (EU) 2015/2283 laid the framework for the application process to market food insects in member states, but potential hazards are still being evaluated. The aim of this study was to investigate samples of edible insect species for the presence of antimicrobial-resistant and Shiga toxin-producing <i>Escherichia coli</i> (STEC). Twenty-one <i>E. coli</i> isolates, recovered from samples of five different edible insect species, were subjected to antimicrobial susceptibility testing, PCR-based phylotyping, and macrorestriction analysis. The presence of genes associated with antimicrobial resistance or virulence, including <i>stx1</i>, <i>stx2,</i> and <i>eae</i>, was investigated by PCR. All isolates were subjected to genome sequencing, multilocus sequence typing, and serotype prediction. The isolates belonged either to phylogenetic group A, comprising mostly commensal <i>E. coli</i>, or group B1. One O178:H7 isolate, recovered from a <i>Zophobas atratus</i> sample, was identified as a STEC. A single isolate was resistant to tetracyclines and carried the <i>tet</i>(B) gene. Overall, this study shows that STEC can be present in edible insects, representing a potential health hazard. In contrast, the low resistance rate among the isolates indicates a low risk for the transmission of antimicrobial-resistant <i>E. coli</i> to consumers.
format	article
author	Anja Müller Diana Seinige Nils T. Grabowski Birte Ahlfeld Min Yue Corinna Kehrenberg
author_facet	Anja Müller Diana Seinige Nils T. Grabowski Birte Ahlfeld Min Yue Corinna Kehrenberg
author_sort	Anja Müller
title	Characterization of <i>Escherichia coli</i> from Edible Insect Species: Detection of Shiga Toxin-Producing Isolate
title_short	Characterization of <i>Escherichia coli</i> from Edible Insect Species: Detection of Shiga Toxin-Producing Isolate
title_full	Characterization of <i>Escherichia coli</i> from Edible Insect Species: Detection of Shiga Toxin-Producing Isolate
title_fullStr	Characterization of <i>Escherichia coli</i> from Edible Insect Species: Detection of Shiga Toxin-Producing Isolate
title_full_unstemmed	Characterization of <i>Escherichia coli</i> from Edible Insect Species: Detection of Shiga Toxin-Producing Isolate
title_sort	characterization of <i>escherichia coli</i> from edible insect species: detection of shiga toxin-producing isolate
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/b3701ebda792412f9f23dbf3a5e1da68
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Characterization of <i>Escherichia coli</i> from Edible Insect Species: Detection of Shiga Toxin-Producing Isolate

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