Exoproteomic profiling uncovers critical determinants for virulence of livestock-associated and human-originated Staphylococcus aureus ST398 strains

Staphylococcus aureus: with the sequence type (ST) 398 was previously associated with livestock carriage. However, in recent years livestock-independent S. aureus ST398 has emerged, representing a potential health risk for humans especially in nosocomial settings. Judged by whole-genome sequencing a...

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Autores principales:	Xin Zhao, Monika A. Chlebowicz-Flissikowska, Min Wang, Elias Vera Murguia, Anne de Jong, Dörte Becher, Sandra Maaß, Girbe Buist, Jan Maarten van Dijl
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2020
Materias:	s. aureus st398 exoproteome virulence galleria mellonella hela cells Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/b377714e301d423c9ec86ea29d9732e7
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spelling	oai:doaj.org-article:b377714e301d423c9ec86ea29d9732e72021-11-17T14:21:58ZExoproteomic profiling uncovers critical determinants for virulence of livestock-associated and human-originated Staphylococcus aureus ST398 strains2150-55942150-560810.1080/21505594.2020.1793525https://doaj.org/article/b377714e301d423c9ec86ea29d9732e72020-12-01T00:00:00Zhttp://dx.doi.org/10.1080/21505594.2020.1793525https://doaj.org/toc/2150-5594https://doaj.org/toc/2150-5608Staphylococcus aureus: with the sequence type (ST) 398 was previously associated with livestock carriage. However, in recent years livestock-independent S. aureus ST398 has emerged, representing a potential health risk for humans especially in nosocomial settings. Judged by whole-genome sequencing analyses, the livestock- and human originated strains belong to two different S. aureus ST398 clades but, to date, it was not known to what extent these clades differ in terms of actual virulence. Therefore, the objective of this study was to profile the exoproteomes of 30 representative S. aureus ST398 strains by mass spectrometry, to assess clade-specific differences in virulence factor secretion, and to correlate the identified proteins and their relative abundance to the strains’ actual virulence. Although the human-originated strains are more heterogeneous at the genome level, our observations show that they are more homogeneous in terms of virulence factor production than the livestock-associated strains. To assess differences in virulence, infection models based on larvae of the wax moth Galleria mellonella and the human HeLa cell line were applied. Correlation of the exoproteome data to larval killing and toxicity toward HeLa cells uncovered critical roles of the staphylococcal Sbi, SpA, SCIN and CHIPS proteins in virulence. These findings were validated by showing that sbi or spa mutant bacteria are attenuated in G. mellonella and that the purified SCIN and CHIPS proteins are toxic for HeLa cells. Altogether, we show that exoproteome profiling allows the identification of critical determinants for virulence of livestock-associated and human-originated S. aureus ST398 strains.Xin ZhaoMonika A. Chlebowicz-FlissikowskaMin WangElias Vera MurguiaAnne de JongDörte BecherSandra MaaßGirbe BuistJan Maarten van DijlTaylor & Francis Grouparticles. aureusst398exoproteomevirulencegalleria mellonellahela cellsInfectious and parasitic diseasesRC109-216ENVirulence, Vol 11, Iss 1, Pp 947-963 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	s. aureus st398 exoproteome virulence galleria mellonella hela cells Infectious and parasitic diseases RC109-216
spellingShingle	s. aureus st398 exoproteome virulence galleria mellonella hela cells Infectious and parasitic diseases RC109-216 Xin Zhao Monika A. Chlebowicz-Flissikowska Min Wang Elias Vera Murguia Anne de Jong Dörte Becher Sandra Maaß Girbe Buist Jan Maarten van Dijl Exoproteomic profiling uncovers critical determinants for virulence of livestock-associated and human-originated Staphylococcus aureus ST398 strains
description	Staphylococcus aureus: with the sequence type (ST) 398 was previously associated with livestock carriage. However, in recent years livestock-independent S. aureus ST398 has emerged, representing a potential health risk for humans especially in nosocomial settings. Judged by whole-genome sequencing analyses, the livestock- and human originated strains belong to two different S. aureus ST398 clades but, to date, it was not known to what extent these clades differ in terms of actual virulence. Therefore, the objective of this study was to profile the exoproteomes of 30 representative S. aureus ST398 strains by mass spectrometry, to assess clade-specific differences in virulence factor secretion, and to correlate the identified proteins and their relative abundance to the strains’ actual virulence. Although the human-originated strains are more heterogeneous at the genome level, our observations show that they are more homogeneous in terms of virulence factor production than the livestock-associated strains. To assess differences in virulence, infection models based on larvae of the wax moth Galleria mellonella and the human HeLa cell line were applied. Correlation of the exoproteome data to larval killing and toxicity toward HeLa cells uncovered critical roles of the staphylococcal Sbi, SpA, SCIN and CHIPS proteins in virulence. These findings were validated by showing that sbi or spa mutant bacteria are attenuated in G. mellonella and that the purified SCIN and CHIPS proteins are toxic for HeLa cells. Altogether, we show that exoproteome profiling allows the identification of critical determinants for virulence of livestock-associated and human-originated S. aureus ST398 strains.
format	article
author	Xin Zhao Monika A. Chlebowicz-Flissikowska Min Wang Elias Vera Murguia Anne de Jong Dörte Becher Sandra Maaß Girbe Buist Jan Maarten van Dijl
author_facet	Xin Zhao Monika A. Chlebowicz-Flissikowska Min Wang Elias Vera Murguia Anne de Jong Dörte Becher Sandra Maaß Girbe Buist Jan Maarten van Dijl
author_sort	Xin Zhao
title	Exoproteomic profiling uncovers critical determinants for virulence of livestock-associated and human-originated Staphylococcus aureus ST398 strains
title_short	Exoproteomic profiling uncovers critical determinants for virulence of livestock-associated and human-originated Staphylococcus aureus ST398 strains
title_full	Exoproteomic profiling uncovers critical determinants for virulence of livestock-associated and human-originated Staphylococcus aureus ST398 strains
title_fullStr	Exoproteomic profiling uncovers critical determinants for virulence of livestock-associated and human-originated Staphylococcus aureus ST398 strains
title_full_unstemmed	Exoproteomic profiling uncovers critical determinants for virulence of livestock-associated and human-originated Staphylococcus aureus ST398 strains
title_sort	exoproteomic profiling uncovers critical determinants for virulence of livestock-associated and human-originated staphylococcus aureus st398 strains
publisher	Taylor & Francis Group
publishDate	2020
url	https://doaj.org/article/b377714e301d423c9ec86ea29d9732e7
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Exoproteomic profiling uncovers critical determinants for virulence of livestock-associated and human-originated Staphylococcus aureus ST398 strains

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