Effects of microgravity exposure and fructo-oligosaccharide ingestion on the proteome of soleus and extensor digitorum longus muscles in developing mice

Abstract Short-chain fatty acids produced by the gut bacterial fermentation of non-digestible carbohydrates, e.g., fructo-oligosaccharide (FOS), contribute to the maintenance of skeletal muscle mass and oxidative metabolic capacity. We evaluated the effect of FOS ingestion on protein expression of s...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Takashi Ohira, Yoko Ino, Yayoi Kimura, Yusuke Nakai, Ayuko Kimura, Yoichi Kurata, Hiroyuki Kagawa, Mitsuo Kimura, Kenji Egashira, Chie Matsuda, Yoshinobu Ohira, Satoshi Furukawa, Hisashi Hirano
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Biotechnology TP248.13-248.65 Physiology QP1-981
Acceso en línea:	https://doaj.org/article/b3782734408b463d92e0ab8c01d1b559
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:b3782734408b463d92e0ab8c01d1b559
record_format	dspace
spelling	oai:doaj.org-article:b3782734408b463d92e0ab8c01d1b5592021-12-02T18:02:24ZEffects of microgravity exposure and fructo-oligosaccharide ingestion on the proteome of soleus and extensor digitorum longus muscles in developing mice10.1038/s41526-021-00164-62373-8065https://doaj.org/article/b3782734408b463d92e0ab8c01d1b5592021-09-01T00:00:00Zhttps://doi.org/10.1038/s41526-021-00164-6https://doaj.org/toc/2373-8065Abstract Short-chain fatty acids produced by the gut bacterial fermentation of non-digestible carbohydrates, e.g., fructo-oligosaccharide (FOS), contribute to the maintenance of skeletal muscle mass and oxidative metabolic capacity. We evaluated the effect of FOS ingestion on protein expression of soleus (Sol) and extensor digitorum longus muscles in mice exposed to microgravity (μ-g). Twelve 9-week-old male C57BL/6J mice were raised individually on the International Space Station under μ-g or artificial 1-g and fed a diet with or without FOS (n = 3/group). Regardless of FOS ingestion, the absolute wet weights of both muscles tended to decrease, and the fiber phenotype in Sol muscles shifted toward fast-twitch type following μ-g exposure. However, FOS ingestion tended to mitigate the μ-g-exposure-related decrease in oxidative metabolism and enhance glutathione redox detoxification in Sol muscles. These results indicate that FOS ingestion mildly suppresses metabolic changes and oxidative stress in antigravity Sol muscles during spaceflight.Takashi OhiraYoko InoYayoi KimuraYusuke NakaiAyuko KimuraYoichi KurataHiroyuki KagawaMitsuo KimuraKenji EgashiraChie MatsudaYoshinobu OhiraSatoshi FurukawaHisashi HiranoNature PortfolioarticleBiotechnologyTP248.13-248.65PhysiologyQP1-981ENnpj Microgravity, Vol 7, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biotechnology TP248.13-248.65 Physiology QP1-981
spellingShingle	Biotechnology TP248.13-248.65 Physiology QP1-981 Takashi Ohira Yoko Ino Yayoi Kimura Yusuke Nakai Ayuko Kimura Yoichi Kurata Hiroyuki Kagawa Mitsuo Kimura Kenji Egashira Chie Matsuda Yoshinobu Ohira Satoshi Furukawa Hisashi Hirano Effects of microgravity exposure and fructo-oligosaccharide ingestion on the proteome of soleus and extensor digitorum longus muscles in developing mice
description	Abstract Short-chain fatty acids produced by the gut bacterial fermentation of non-digestible carbohydrates, e.g., fructo-oligosaccharide (FOS), contribute to the maintenance of skeletal muscle mass and oxidative metabolic capacity. We evaluated the effect of FOS ingestion on protein expression of soleus (Sol) and extensor digitorum longus muscles in mice exposed to microgravity (μ-g). Twelve 9-week-old male C57BL/6J mice were raised individually on the International Space Station under μ-g or artificial 1-g and fed a diet with or without FOS (n = 3/group). Regardless of FOS ingestion, the absolute wet weights of both muscles tended to decrease, and the fiber phenotype in Sol muscles shifted toward fast-twitch type following μ-g exposure. However, FOS ingestion tended to mitigate the μ-g-exposure-related decrease in oxidative metabolism and enhance glutathione redox detoxification in Sol muscles. These results indicate that FOS ingestion mildly suppresses metabolic changes and oxidative stress in antigravity Sol muscles during spaceflight.
format	article
author	Takashi Ohira Yoko Ino Yayoi Kimura Yusuke Nakai Ayuko Kimura Yoichi Kurata Hiroyuki Kagawa Mitsuo Kimura Kenji Egashira Chie Matsuda Yoshinobu Ohira Satoshi Furukawa Hisashi Hirano
author_facet	Takashi Ohira Yoko Ino Yayoi Kimura Yusuke Nakai Ayuko Kimura Yoichi Kurata Hiroyuki Kagawa Mitsuo Kimura Kenji Egashira Chie Matsuda Yoshinobu Ohira Satoshi Furukawa Hisashi Hirano
author_sort	Takashi Ohira
title	Effects of microgravity exposure and fructo-oligosaccharide ingestion on the proteome of soleus and extensor digitorum longus muscles in developing mice
title_short	Effects of microgravity exposure and fructo-oligosaccharide ingestion on the proteome of soleus and extensor digitorum longus muscles in developing mice
title_full	Effects of microgravity exposure and fructo-oligosaccharide ingestion on the proteome of soleus and extensor digitorum longus muscles in developing mice
title_fullStr	Effects of microgravity exposure and fructo-oligosaccharide ingestion on the proteome of soleus and extensor digitorum longus muscles in developing mice
title_full_unstemmed	Effects of microgravity exposure and fructo-oligosaccharide ingestion on the proteome of soleus and extensor digitorum longus muscles in developing mice
title_sort	effects of microgravity exposure and fructo-oligosaccharide ingestion on the proteome of soleus and extensor digitorum longus muscles in developing mice
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/b3782734408b463d92e0ab8c01d1b559
work_keys_str_mv	AT takashiohira effectsofmicrogravityexposureandfructooligosaccharideingestionontheproteomeofsoleusandextensordigitorumlongusmusclesindevelopingmice AT yokoino effectsofmicrogravityexposureandfructooligosaccharideingestionontheproteomeofsoleusandextensordigitorumlongusmusclesindevelopingmice AT yayoikimura effectsofmicrogravityexposureandfructooligosaccharideingestionontheproteomeofsoleusandextensordigitorumlongusmusclesindevelopingmice AT yusukenakai effectsofmicrogravityexposureandfructooligosaccharideingestionontheproteomeofsoleusandextensordigitorumlongusmusclesindevelopingmice AT ayukokimura effectsofmicrogravityexposureandfructooligosaccharideingestionontheproteomeofsoleusandextensordigitorumlongusmusclesindevelopingmice AT yoichikurata effectsofmicrogravityexposureandfructooligosaccharideingestionontheproteomeofsoleusandextensordigitorumlongusmusclesindevelopingmice AT hiroyukikagawa effectsofmicrogravityexposureandfructooligosaccharideingestionontheproteomeofsoleusandextensordigitorumlongusmusclesindevelopingmice AT mitsuokimura effectsofmicrogravityexposureandfructooligosaccharideingestionontheproteomeofsoleusandextensordigitorumlongusmusclesindevelopingmice AT kenjiegashira effectsofmicrogravityexposureandfructooligosaccharideingestionontheproteomeofsoleusandextensordigitorumlongusmusclesindevelopingmice AT chiematsuda effectsofmicrogravityexposureandfructooligosaccharideingestionontheproteomeofsoleusandextensordigitorumlongusmusclesindevelopingmice AT yoshinobuohira effectsofmicrogravityexposureandfructooligosaccharideingestionontheproteomeofsoleusandextensordigitorumlongusmusclesindevelopingmice AT satoshifurukawa effectsofmicrogravityexposureandfructooligosaccharideingestionontheproteomeofsoleusandextensordigitorumlongusmusclesindevelopingmice AT hisashihirano effectsofmicrogravityexposureandfructooligosaccharideingestionontheproteomeofsoleusandextensordigitorumlongusmusclesindevelopingmice
_version_	1718378891108679680

Effects of microgravity exposure and fructo-oligosaccharide ingestion on the proteome of soleus and extensor digitorum longus muscles in developing mice

Ejemplares similares