Compartmentalized 3D Tissue Culture Arrays under Controlled Microfluidic Delivery

Abstract We demonstrate an in vitro microfluidic cell culture platform that consists of periodic 3D hydrogel compartments with controllable shapes. The microchip is composed of approximately 500 discontinuous collagen gel compartments locally patterned in between PDMS pillars, separated by microflui...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Burcu Gumuscu, Hugo J. Albers, Albert van den Berg, Jan C. T. Eijkel, Andries D. van der Meer
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b37e46c92e784e2f9d2c167bb49b230a
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:b37e46c92e784e2f9d2c167bb49b230a
record_format	dspace
spelling	oai:doaj.org-article:b37e46c92e784e2f9d2c167bb49b230a2021-12-02T15:06:15ZCompartmentalized 3D Tissue Culture Arrays under Controlled Microfluidic Delivery10.1038/s41598-017-01944-52045-2322https://doaj.org/article/b37e46c92e784e2f9d2c167bb49b230a2017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01944-5https://doaj.org/toc/2045-2322Abstract We demonstrate an in vitro microfluidic cell culture platform that consists of periodic 3D hydrogel compartments with controllable shapes. The microchip is composed of approximately 500 discontinuous collagen gel compartments locally patterned in between PDMS pillars, separated by microfluidic channels. The typical volume of each compartment is 7.5 nanoliters. The compartmentalized design of the microchip and continuous fluid delivery enable long-term culturing of Caco-2 human intestine cells. We found that the cells started to spontaneously grow into 3D folds on day 3 of the culture. On day 8, Caco-2 cells were co-cultured for 36 hours under microfluidic perfusion with intestinal bacteria (E. coli) which did not overgrow in the system, and adhered to the Caco-2 cells without affecting cell viability. Continuous perfusion enabled the preliminary evaluation of drug effects by treating the co-culture of Caco-2 and E. coli with 34 µg ml−1 chloramphenicol during 36 hours, resulting in the death of the bacteria. Caco-2 cells were also cultured in different compartment geometries with large and small hydrogel interfaces, leading to differences in proliferation and cell spreading profile of Caco-2 cells. The presented approach of compartmentalized cell culture with facile microfluidic control can substantially increase the throughput of in vitro drug screening in the future.Burcu GumuscuHugo J. AlbersAlbert van den BergJan C. T. EijkelAndries D. van der MeerNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Burcu Gumuscu Hugo J. Albers Albert van den Berg Jan C. T. Eijkel Andries D. van der Meer Compartmentalized 3D Tissue Culture Arrays under Controlled Microfluidic Delivery
description	Abstract We demonstrate an in vitro microfluidic cell culture platform that consists of periodic 3D hydrogel compartments with controllable shapes. The microchip is composed of approximately 500 discontinuous collagen gel compartments locally patterned in between PDMS pillars, separated by microfluidic channels. The typical volume of each compartment is 7.5 nanoliters. The compartmentalized design of the microchip and continuous fluid delivery enable long-term culturing of Caco-2 human intestine cells. We found that the cells started to spontaneously grow into 3D folds on day 3 of the culture. On day 8, Caco-2 cells were co-cultured for 36 hours under microfluidic perfusion with intestinal bacteria (E. coli) which did not overgrow in the system, and adhered to the Caco-2 cells without affecting cell viability. Continuous perfusion enabled the preliminary evaluation of drug effects by treating the co-culture of Caco-2 and E. coli with 34 µg ml−1 chloramphenicol during 36 hours, resulting in the death of the bacteria. Caco-2 cells were also cultured in different compartment geometries with large and small hydrogel interfaces, leading to differences in proliferation and cell spreading profile of Caco-2 cells. The presented approach of compartmentalized cell culture with facile microfluidic control can substantially increase the throughput of in vitro drug screening in the future.
format	article
author	Burcu Gumuscu Hugo J. Albers Albert van den Berg Jan C. T. Eijkel Andries D. van der Meer
author_facet	Burcu Gumuscu Hugo J. Albers Albert van den Berg Jan C. T. Eijkel Andries D. van der Meer
author_sort	Burcu Gumuscu
title	Compartmentalized 3D Tissue Culture Arrays under Controlled Microfluidic Delivery
title_short	Compartmentalized 3D Tissue Culture Arrays under Controlled Microfluidic Delivery
title_full	Compartmentalized 3D Tissue Culture Arrays under Controlled Microfluidic Delivery
title_fullStr	Compartmentalized 3D Tissue Culture Arrays under Controlled Microfluidic Delivery
title_full_unstemmed	Compartmentalized 3D Tissue Culture Arrays under Controlled Microfluidic Delivery
title_sort	compartmentalized 3d tissue culture arrays under controlled microfluidic delivery
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/b37e46c92e784e2f9d2c167bb49b230a
work_keys_str_mv	AT burcugumuscu compartmentalized3dtissueculturearraysundercontrolledmicrofluidicdelivery AT hugojalbers compartmentalized3dtissueculturearraysundercontrolledmicrofluidicdelivery AT albertvandenberg compartmentalized3dtissueculturearraysundercontrolledmicrofluidicdelivery AT jancteijkel compartmentalized3dtissueculturearraysundercontrolledmicrofluidicdelivery AT andriesdvandermeer compartmentalized3dtissueculturearraysundercontrolledmicrofluidicdelivery
_version_	1718388537677578240

Compartmentalized 3D Tissue Culture Arrays under Controlled Microfluidic Delivery

Ejemplares similares