TLR3 and TLR7 modulate IgE production in antigen induced pulmonary inflammation via influencing IL-4 expression in immune organs.
<h4>Background</h4>Toll-like receptors (TLRs) as pattern recognition receptors, participate in both innate and adaptive immune responses, and seem to play an important role in the pathogenesis of asthma. This study aimed to identify key TLRs involved in antigen induced pulmonary inflamma...
Guardado en:
| Autores principales: | , , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Public Library of Science (PLoS)
2011
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/b385b256763c40caaca4b5cb65b2bd80 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:b385b256763c40caaca4b5cb65b2bd80 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:b385b256763c40caaca4b5cb65b2bd802021-11-18T06:58:09ZTLR3 and TLR7 modulate IgE production in antigen induced pulmonary inflammation via influencing IL-4 expression in immune organs.1932-620310.1371/journal.pone.0017252https://doaj.org/article/b385b256763c40caaca4b5cb65b2bd802011-02-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21364926/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Toll-like receptors (TLRs) as pattern recognition receptors, participate in both innate and adaptive immune responses, and seem to play an important role in the pathogenesis of asthma. This study aimed to identify key TLRs involved in antigen induced pulmonary inflammation (AIPI), a rat model for asthma, and to explore the role of TLRs in the disease development.<h4>Methods and findings</h4>E3 rats were sensitized with ovalbumin (OVA)/alum intraperitoneally and intranasally challenged with OVA to induce AIPI model. TLR1-9 and cytokine mRNA expression in spleen, lung and mediastinal lymph node (mLN) tissues were screened by quantitative real-time polymerase chain reaction. TLR7 expression was found to be significantly down-regulated in spleen while TLR3 and TLR8 expression was up-regulated in mLN of AIPI rats. Furthermore, imiquimod (a ligand of TLR7) and TLR3 specific short-hairpin RNA plasmid for RNA interference were administrated, respectively, in vivo to AIPI rats to observe their effects on the disease by assessing various asthmatic parameters. The numbers of total cells, eosinophils, macrophages and lymphocytes were counted according to differential morphology in bronchoalveolar lavage fluid. Serum IgE and OVA specific IgG(1) concentration was detected by enzyme-linked immunosorbent assay. The results showed that both TLR7 ligand treatment and TLR3 RNAi in vivo decreased serum IgE level and interleukin-4 mRNA expression.<h4>Conclusion/significance</h4>TLR3 in mLN and TLR7 in spleen both systemically modulate disease development in AIPI rats via altering serum IgE concentration relevant to Th2 responses. And these findings may provide an important clue for further research in the asthma pathogenesis and suggest a new remedy for asthma treatment.Liesu MengXiaojing HeWenhua ZhuXudong YangCongshan JiangQingzhu SunM BSimeng ZhangQian XueXinfang XieShemin LuShemin LuPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 2, p e17252 (2011) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q Liesu Meng Xiaojing He Wenhua Zhu Xudong Yang Congshan Jiang Qingzhu Sun M B Simeng Zhang Qian Xue Xinfang Xie Shemin Lu Shemin Lu TLR3 and TLR7 modulate IgE production in antigen induced pulmonary inflammation via influencing IL-4 expression in immune organs. |
| description |
<h4>Background</h4>Toll-like receptors (TLRs) as pattern recognition receptors, participate in both innate and adaptive immune responses, and seem to play an important role in the pathogenesis of asthma. This study aimed to identify key TLRs involved in antigen induced pulmonary inflammation (AIPI), a rat model for asthma, and to explore the role of TLRs in the disease development.<h4>Methods and findings</h4>E3 rats were sensitized with ovalbumin (OVA)/alum intraperitoneally and intranasally challenged with OVA to induce AIPI model. TLR1-9 and cytokine mRNA expression in spleen, lung and mediastinal lymph node (mLN) tissues were screened by quantitative real-time polymerase chain reaction. TLR7 expression was found to be significantly down-regulated in spleen while TLR3 and TLR8 expression was up-regulated in mLN of AIPI rats. Furthermore, imiquimod (a ligand of TLR7) and TLR3 specific short-hairpin RNA plasmid for RNA interference were administrated, respectively, in vivo to AIPI rats to observe their effects on the disease by assessing various asthmatic parameters. The numbers of total cells, eosinophils, macrophages and lymphocytes were counted according to differential morphology in bronchoalveolar lavage fluid. Serum IgE and OVA specific IgG(1) concentration was detected by enzyme-linked immunosorbent assay. The results showed that both TLR7 ligand treatment and TLR3 RNAi in vivo decreased serum IgE level and interleukin-4 mRNA expression.<h4>Conclusion/significance</h4>TLR3 in mLN and TLR7 in spleen both systemically modulate disease development in AIPI rats via altering serum IgE concentration relevant to Th2 responses. And these findings may provide an important clue for further research in the asthma pathogenesis and suggest a new remedy for asthma treatment. |
| format |
article |
| author |
Liesu Meng Xiaojing He Wenhua Zhu Xudong Yang Congshan Jiang Qingzhu Sun M B Simeng Zhang Qian Xue Xinfang Xie Shemin Lu Shemin Lu |
| author_facet |
Liesu Meng Xiaojing He Wenhua Zhu Xudong Yang Congshan Jiang Qingzhu Sun M B Simeng Zhang Qian Xue Xinfang Xie Shemin Lu Shemin Lu |
| author_sort |
Liesu Meng |
| title |
TLR3 and TLR7 modulate IgE production in antigen induced pulmonary inflammation via influencing IL-4 expression in immune organs. |
| title_short |
TLR3 and TLR7 modulate IgE production in antigen induced pulmonary inflammation via influencing IL-4 expression in immune organs. |
| title_full |
TLR3 and TLR7 modulate IgE production in antigen induced pulmonary inflammation via influencing IL-4 expression in immune organs. |
| title_fullStr |
TLR3 and TLR7 modulate IgE production in antigen induced pulmonary inflammation via influencing IL-4 expression in immune organs. |
| title_full_unstemmed |
TLR3 and TLR7 modulate IgE production in antigen induced pulmonary inflammation via influencing IL-4 expression in immune organs. |
| title_sort |
tlr3 and tlr7 modulate ige production in antigen induced pulmonary inflammation via influencing il-4 expression in immune organs. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2011 |
| url |
https://doaj.org/article/b385b256763c40caaca4b5cb65b2bd80 |
| work_keys_str_mv |
AT liesumeng tlr3andtlr7modulateigeproductioninantigeninducedpulmonaryinflammationviainfluencingil4expressioninimmuneorgans AT xiaojinghe tlr3andtlr7modulateigeproductioninantigeninducedpulmonaryinflammationviainfluencingil4expressioninimmuneorgans AT wenhuazhu tlr3andtlr7modulateigeproductioninantigeninducedpulmonaryinflammationviainfluencingil4expressioninimmuneorgans AT xudongyang tlr3andtlr7modulateigeproductioninantigeninducedpulmonaryinflammationviainfluencingil4expressioninimmuneorgans AT congshanjiang tlr3andtlr7modulateigeproductioninantigeninducedpulmonaryinflammationviainfluencingil4expressioninimmuneorgans AT qingzhusun tlr3andtlr7modulateigeproductioninantigeninducedpulmonaryinflammationviainfluencingil4expressioninimmuneorgans AT mb tlr3andtlr7modulateigeproductioninantigeninducedpulmonaryinflammationviainfluencingil4expressioninimmuneorgans AT simengzhang tlr3andtlr7modulateigeproductioninantigeninducedpulmonaryinflammationviainfluencingil4expressioninimmuneorgans AT qianxue tlr3andtlr7modulateigeproductioninantigeninducedpulmonaryinflammationviainfluencingil4expressioninimmuneorgans AT xinfangxie tlr3andtlr7modulateigeproductioninantigeninducedpulmonaryinflammationviainfluencingil4expressioninimmuneorgans AT sheminlu tlr3andtlr7modulateigeproductioninantigeninducedpulmonaryinflammationviainfluencingil4expressioninimmuneorgans AT sheminlu tlr3andtlr7modulateigeproductioninantigeninducedpulmonaryinflammationviainfluencingil4expressioninimmuneorgans |
| _version_ |
1718424126923735040 |