Research on oral microbiota of monozygotic twins with discordant caries experience - in vitro and in vivo study

Abstract Oral microbiome is potentially correlated with many diseases, such as dental caries, periodontitis, oral cancer and some systemic diseases. Twin model, as an effective method for studying human microbiota, is widely used in research of relationship between oral microbiota and dental caries....

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Autores principales:	Hongle Wu, Benhua Zeng, Bolei Li, Biao Ren, Jianhua Zhao, Mingyun Li, Xian Peng, Mingye Feng, Jiyao Li, Hong Wei, Lei Cheng, Xuedong Zhou
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b388081efad44cda958c7cecc9068001
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spelling	oai:doaj.org-article:b388081efad44cda958c7cecc90680012021-12-02T16:08:01ZResearch on oral microbiota of monozygotic twins with discordant caries experience - in vitro and in vivo study10.1038/s41598-018-25636-w2045-2322https://doaj.org/article/b388081efad44cda958c7cecc90680012018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-25636-whttps://doaj.org/toc/2045-2322Abstract Oral microbiome is potentially correlated with many diseases, such as dental caries, periodontitis, oral cancer and some systemic diseases. Twin model, as an effective method for studying human microbiota, is widely used in research of relationship between oral microbiota and dental caries. However, there were few researches focusing on caries discordant twins. In this study, in vitro assays were conducted combined with 16S rRNA sequencing analysis on oral microbiota sampled from twins who presented discordant caries experience and mice model was developed as well. Results showed that oral microbiota from caries-active twin possessed higher metabolic activity and produced more lactic production. 16S rRNA sequencing analysis showed that more than 80% of family taxa could be transferred into gnotobiotic-mice. Key caries-associated genera were significantly different between twins and the same difference in genus level could be found in mice as well (p < 0.05). This study suggested that oral microbiota of twins could be distinguished from each other despite the similarities in genetic make-up, living environment, and lifestyle. The difference in microbiota was applied to develop a mice model which may facilitate the investigation of core microbiota of dental caries.Hongle WuBenhua ZengBolei LiBiao RenJianhua ZhaoMingyun LiXian PengMingye FengJiyao LiHong WeiLei ChengXuedong ZhouNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-8 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hongle Wu Benhua Zeng Bolei Li Biao Ren Jianhua Zhao Mingyun Li Xian Peng Mingye Feng Jiyao Li Hong Wei Lei Cheng Xuedong Zhou Research on oral microbiota of monozygotic twins with discordant caries experience - in vitro and in vivo study
description	Abstract Oral microbiome is potentially correlated with many diseases, such as dental caries, periodontitis, oral cancer and some systemic diseases. Twin model, as an effective method for studying human microbiota, is widely used in research of relationship between oral microbiota and dental caries. However, there were few researches focusing on caries discordant twins. In this study, in vitro assays were conducted combined with 16S rRNA sequencing analysis on oral microbiota sampled from twins who presented discordant caries experience and mice model was developed as well. Results showed that oral microbiota from caries-active twin possessed higher metabolic activity and produced more lactic production. 16S rRNA sequencing analysis showed that more than 80% of family taxa could be transferred into gnotobiotic-mice. Key caries-associated genera were significantly different between twins and the same difference in genus level could be found in mice as well (p < 0.05). This study suggested that oral microbiota of twins could be distinguished from each other despite the similarities in genetic make-up, living environment, and lifestyle. The difference in microbiota was applied to develop a mice model which may facilitate the investigation of core microbiota of dental caries.
format	article
author	Hongle Wu Benhua Zeng Bolei Li Biao Ren Jianhua Zhao Mingyun Li Xian Peng Mingye Feng Jiyao Li Hong Wei Lei Cheng Xuedong Zhou
author_facet	Hongle Wu Benhua Zeng Bolei Li Biao Ren Jianhua Zhao Mingyun Li Xian Peng Mingye Feng Jiyao Li Hong Wei Lei Cheng Xuedong Zhou
author_sort	Hongle Wu
title	Research on oral microbiota of monozygotic twins with discordant caries experience - in vitro and in vivo study
title_short	Research on oral microbiota of monozygotic twins with discordant caries experience - in vitro and in vivo study
title_full	Research on oral microbiota of monozygotic twins with discordant caries experience - in vitro and in vivo study
title_fullStr	Research on oral microbiota of monozygotic twins with discordant caries experience - in vitro and in vivo study
title_full_unstemmed	Research on oral microbiota of monozygotic twins with discordant caries experience - in vitro and in vivo study
title_sort	research on oral microbiota of monozygotic twins with discordant caries experience - in vitro and in vivo study
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/b388081efad44cda958c7cecc9068001
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Research on oral microbiota of monozygotic twins with discordant caries experience - in vitro and in vivo study

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