An artificial triazole backbone linkage provides a split-and-click strategy to bioactive chemically modified CRISPR sgRNA

For CRISPR-Cas9 genome editing, Cas9 protein is guided to its target by single guide (sg) RNA. Here, the authors synthesised sgRNAs via convergent ‘click’ ligation of variable 20-mer RNAs that target the genome and a Cas9-binding 79-mer chimeric RNA/2´-OMe RNA of fixed sequence in a single tube....

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Autores principales:	Lapatrada Taemaitree, Arun Shivalingam, Afaf H. El-Sagheer, Tom Brown
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/b38a6c3af638463480d4a7be7b8e7281
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id	oai:doaj.org-article:b38a6c3af638463480d4a7be7b8e7281
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spelling	oai:doaj.org-article:b38a6c3af638463480d4a7be7b8e72812021-12-02T17:33:22ZAn artificial triazole backbone linkage provides a split-and-click strategy to bioactive chemically modified CRISPR sgRNA10.1038/s41467-019-09600-42041-1723https://doaj.org/article/b38a6c3af638463480d4a7be7b8e72812019-04-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-09600-4https://doaj.org/toc/2041-1723For CRISPR-Cas9 genome editing, Cas9 protein is guided to its target by single guide (sg) RNA. Here, the authors synthesised sgRNAs via convergent ‘click’ ligation of variable 20-mer RNAs that target the genome and a Cas9-binding 79-mer chimeric RNA/2´-OMe RNA of fixed sequence in a single tube.Lapatrada TaemaitreeArun ShivalingamAfaf H. El-SagheerTom BrownNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-8 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Lapatrada Taemaitree Arun Shivalingam Afaf H. El-Sagheer Tom Brown An artificial triazole backbone linkage provides a split-and-click strategy to bioactive chemically modified CRISPR sgRNA
description	For CRISPR-Cas9 genome editing, Cas9 protein is guided to its target by single guide (sg) RNA. Here, the authors synthesised sgRNAs via convergent ‘click’ ligation of variable 20-mer RNAs that target the genome and a Cas9-binding 79-mer chimeric RNA/2´-OMe RNA of fixed sequence in a single tube.
format	article
author	Lapatrada Taemaitree Arun Shivalingam Afaf H. El-Sagheer Tom Brown
author_facet	Lapatrada Taemaitree Arun Shivalingam Afaf H. El-Sagheer Tom Brown
author_sort	Lapatrada Taemaitree
title	An artificial triazole backbone linkage provides a split-and-click strategy to bioactive chemically modified CRISPR sgRNA
title_short	An artificial triazole backbone linkage provides a split-and-click strategy to bioactive chemically modified CRISPR sgRNA
title_full	An artificial triazole backbone linkage provides a split-and-click strategy to bioactive chemically modified CRISPR sgRNA
title_fullStr	An artificial triazole backbone linkage provides a split-and-click strategy to bioactive chemically modified CRISPR sgRNA
title_full_unstemmed	An artificial triazole backbone linkage provides a split-and-click strategy to bioactive chemically modified CRISPR sgRNA
title_sort	artificial triazole backbone linkage provides a split-and-click strategy to bioactive chemically modified crispr sgrna
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/b38a6c3af638463480d4a7be7b8e7281
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An artificial triazole backbone linkage provides a split-and-click strategy to bioactive chemically modified CRISPR sgRNA

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