Pro-inflammatory effects of DEHP in SGBS-derived adipocytes and THP-1 macrophages

Abstract In the member countries of the Organization for Economic Co-operation and Development (OECD), overweight and obesity affect the majority of the population. The use of environmental chemicals, such as the plasticizer DEHP, has largely increased simultaneously with this development. DEHP is a...

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Autores principales:	Kristina Schaedlich, Laura-Sophie Beier, Judith Kolbe, Martin Wabitsch, Jana Ernst
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b38f5004aaab42cd93d2fff27d456468
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spelling	oai:doaj.org-article:b38f5004aaab42cd93d2fff27d4564682021-12-02T14:26:20ZPro-inflammatory effects of DEHP in SGBS-derived adipocytes and THP-1 macrophages10.1038/s41598-021-85119-32045-2322https://doaj.org/article/b38f5004aaab42cd93d2fff27d4564682021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-85119-3https://doaj.org/toc/2045-2322Abstract In the member countries of the Organization for Economic Co-operation and Development (OECD), overweight and obesity affect the majority of the population. The use of environmental chemicals, such as the plasticizer DEHP, has largely increased simultaneously with this development. DEHP is an "obesogen" that interferes with normal adipocyte differentiation and energy homeostasis. Obesity in turn is accompanied by chronic low-grade adipose tissue inflammation, leading to metabolic disorders such as type II diabetes. The main actors in adipose tissue inflammation are adipocytes and macrophages. However, the impact of DEHP on adipose tissue inflammation and the crosstalk between adipocytes and macrophages are unknown and the subjects of the current study. The influence of DEHP on inflammation was investigated in human Simpson–Golabi–Behmel syndrome (SGBS)-derived adipocytes and human THP-1 macrophages. The proinflammatory markers IL8, MCP1, IL1β, TNFα and others were measured (qRT-PCR, ELISA) in SGBS-derived adipocytes treated with DEHP [day 0 (d0)–d4; 50 µg/ml] and THP-1 macrophages cultured with conditioned medium (CM) from DEHP-treated adipocytes (SGBS-CM) (from d4 and d8). DEHP exposure led to a proinflammatory state in SGBS-derived adipocytes (e.g., increased secretion of IL8 and MCP1). Surprisingly, exposure of THP-1 macrophages to SGBS-CM did not show DEHP-induced effects. However, we demonstrated that medium containing (pre)adipocyte-secreted factors had a significant impact on the expression and secretion of macrophage and inflammatory markers in THP-1 macrophages in general and led to the significantly increased accumulation of intracellular lipid droplets.Kristina SchaedlichLaura-Sophie BeierJudith KolbeMartin WabitschJana ErnstNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kristina Schaedlich Laura-Sophie Beier Judith Kolbe Martin Wabitsch Jana Ernst Pro-inflammatory effects of DEHP in SGBS-derived adipocytes and THP-1 macrophages
description	Abstract In the member countries of the Organization for Economic Co-operation and Development (OECD), overweight and obesity affect the majority of the population. The use of environmental chemicals, such as the plasticizer DEHP, has largely increased simultaneously with this development. DEHP is an "obesogen" that interferes with normal adipocyte differentiation and energy homeostasis. Obesity in turn is accompanied by chronic low-grade adipose tissue inflammation, leading to metabolic disorders such as type II diabetes. The main actors in adipose tissue inflammation are adipocytes and macrophages. However, the impact of DEHP on adipose tissue inflammation and the crosstalk between adipocytes and macrophages are unknown and the subjects of the current study. The influence of DEHP on inflammation was investigated in human Simpson–Golabi–Behmel syndrome (SGBS)-derived adipocytes and human THP-1 macrophages. The proinflammatory markers IL8, MCP1, IL1β, TNFα and others were measured (qRT-PCR, ELISA) in SGBS-derived adipocytes treated with DEHP [day 0 (d0)–d4; 50 µg/ml] and THP-1 macrophages cultured with conditioned medium (CM) from DEHP-treated adipocytes (SGBS-CM) (from d4 and d8). DEHP exposure led to a proinflammatory state in SGBS-derived adipocytes (e.g., increased secretion of IL8 and MCP1). Surprisingly, exposure of THP-1 macrophages to SGBS-CM did not show DEHP-induced effects. However, we demonstrated that medium containing (pre)adipocyte-secreted factors had a significant impact on the expression and secretion of macrophage and inflammatory markers in THP-1 macrophages in general and led to the significantly increased accumulation of intracellular lipid droplets.
format	article
author	Kristina Schaedlich Laura-Sophie Beier Judith Kolbe Martin Wabitsch Jana Ernst
author_facet	Kristina Schaedlich Laura-Sophie Beier Judith Kolbe Martin Wabitsch Jana Ernst
author_sort	Kristina Schaedlich
title	Pro-inflammatory effects of DEHP in SGBS-derived adipocytes and THP-1 macrophages
title_short	Pro-inflammatory effects of DEHP in SGBS-derived adipocytes and THP-1 macrophages
title_full	Pro-inflammatory effects of DEHP in SGBS-derived adipocytes and THP-1 macrophages
title_fullStr	Pro-inflammatory effects of DEHP in SGBS-derived adipocytes and THP-1 macrophages
title_full_unstemmed	Pro-inflammatory effects of DEHP in SGBS-derived adipocytes and THP-1 macrophages
title_sort	pro-inflammatory effects of dehp in sgbs-derived adipocytes and thp-1 macrophages
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/b38f5004aaab42cd93d2fff27d456468
work_keys_str_mv	AT kristinaschaedlich proinflammatoryeffectsofdehpinsgbsderivedadipocytesandthp1macrophages AT laurasophiebeier proinflammatoryeffectsofdehpinsgbsderivedadipocytesandthp1macrophages AT judithkolbe proinflammatoryeffectsofdehpinsgbsderivedadipocytesandthp1macrophages AT martinwabitsch proinflammatoryeffectsofdehpinsgbsderivedadipocytesandthp1macrophages AT janaernst proinflammatoryeffectsofdehpinsgbsderivedadipocytesandthp1macrophages
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Pro-inflammatory effects of DEHP in SGBS-derived adipocytes and THP-1 macrophages

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