Phytohormonal crosstalk modulates the expression of miR166/165s, target Class III HD-ZIPs, and KANADI genes during root growth in Arabidopsis thaliana

Abstract Both phytohormones and non-coding microRNAs (miRNAs) play important role in root development in Arabidopsis thaliana. Mature miR166/165 s, which are derived from precursor transcripts of concerned genes, regulate developmental processes, including leaf and root patterning, by targeting Clas...

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Autores principales:	Archita Singh, Shradha Roy, Sharmila Singh, Shabari Sarkar Das, Vibhav Gautam, Sandeep Yadav, Ashutosh Kumar, Alka Singh, Sukanya Samantha, Ananda K. Sarkar
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b393be25f10f4442bf778031adca23ff
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spelling	oai:doaj.org-article:b393be25f10f4442bf778031adca23ff2021-12-02T12:30:44ZPhytohormonal crosstalk modulates the expression of miR166/165s, target Class III HD-ZIPs, and KANADI genes during root growth in Arabidopsis thaliana10.1038/s41598-017-03632-w2045-2322https://doaj.org/article/b393be25f10f4442bf778031adca23ff2017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03632-whttps://doaj.org/toc/2045-2322Abstract Both phytohormones and non-coding microRNAs (miRNAs) play important role in root development in Arabidopsis thaliana. Mature miR166/165 s, which are derived from precursor transcripts of concerned genes, regulate developmental processes, including leaf and root patterning, by targeting Class III HOMEODOMAIN LEUCINE-ZIPPER (HD-ZIP III) transcription factors (TFs). However, their regulation through hormones remained poorly understood. Here, we show that several phytohormones dynamically regulate the spatio-temporal expression pattern of miR166/165 and target HD-ZIP IIIs in developing roots. Hormone signaling pathway mutants show differential expression pattern of miR166/165, providing further genetic evidence for multilayered regulation of these genes through phytohormones. We further show that a crosstalk of at least six different phytohormones regulate the miR166/165, their target HD-ZIP IIIs, and KANADI (KANs). Our results suggest that HD-ZIP IIIs mediated root development is modulated both transcriptionally through phytohormones and KANs, and post-transcriptionally by miR166/165 that in turn are also regulated by the phytohormonal crosstalk.Archita SinghShradha RoySharmila SinghShabari Sarkar DasVibhav GautamSandeep YadavAshutosh KumarAlka SinghSukanya SamanthaAnanda K. SarkarNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Archita Singh Shradha Roy Sharmila Singh Shabari Sarkar Das Vibhav Gautam Sandeep Yadav Ashutosh Kumar Alka Singh Sukanya Samantha Ananda K. Sarkar Phytohormonal crosstalk modulates the expression of miR166/165s, target Class III HD-ZIPs, and KANADI genes during root growth in Arabidopsis thaliana
description	Abstract Both phytohormones and non-coding microRNAs (miRNAs) play important role in root development in Arabidopsis thaliana. Mature miR166/165 s, which are derived from precursor transcripts of concerned genes, regulate developmental processes, including leaf and root patterning, by targeting Class III HOMEODOMAIN LEUCINE-ZIPPER (HD-ZIP III) transcription factors (TFs). However, their regulation through hormones remained poorly understood. Here, we show that several phytohormones dynamically regulate the spatio-temporal expression pattern of miR166/165 and target HD-ZIP IIIs in developing roots. Hormone signaling pathway mutants show differential expression pattern of miR166/165, providing further genetic evidence for multilayered regulation of these genes through phytohormones. We further show that a crosstalk of at least six different phytohormones regulate the miR166/165, their target HD-ZIP IIIs, and KANADI (KANs). Our results suggest that HD-ZIP IIIs mediated root development is modulated both transcriptionally through phytohormones and KANs, and post-transcriptionally by miR166/165 that in turn are also regulated by the phytohormonal crosstalk.
format	article
author	Archita Singh Shradha Roy Sharmila Singh Shabari Sarkar Das Vibhav Gautam Sandeep Yadav Ashutosh Kumar Alka Singh Sukanya Samantha Ananda K. Sarkar
author_facet	Archita Singh Shradha Roy Sharmila Singh Shabari Sarkar Das Vibhav Gautam Sandeep Yadav Ashutosh Kumar Alka Singh Sukanya Samantha Ananda K. Sarkar
author_sort	Archita Singh
title	Phytohormonal crosstalk modulates the expression of miR166/165s, target Class III HD-ZIPs, and KANADI genes during root growth in Arabidopsis thaliana
title_short	Phytohormonal crosstalk modulates the expression of miR166/165s, target Class III HD-ZIPs, and KANADI genes during root growth in Arabidopsis thaliana
title_full	Phytohormonal crosstalk modulates the expression of miR166/165s, target Class III HD-ZIPs, and KANADI genes during root growth in Arabidopsis thaliana
title_fullStr	Phytohormonal crosstalk modulates the expression of miR166/165s, target Class III HD-ZIPs, and KANADI genes during root growth in Arabidopsis thaliana
title_full_unstemmed	Phytohormonal crosstalk modulates the expression of miR166/165s, target Class III HD-ZIPs, and KANADI genes during root growth in Arabidopsis thaliana
title_sort	phytohormonal crosstalk modulates the expression of mir166/165s, target class iii hd-zips, and kanadi genes during root growth in arabidopsis thaliana
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/b393be25f10f4442bf778031adca23ff
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Phytohormonal crosstalk modulates the expression of miR166/165s, target Class III HD-ZIPs, and KANADI genes during root growth in Arabidopsis thaliana

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