Inducible Enrichment of Osa-miR1432 Confers Rice Bacterial Blight Resistance through Suppressing <i>OsCaML2</i>
MicroRNAs (miRNAs) handle immune response to pathogens by adjusting the function of target genes in plants. However, the experimentally documented miRNA/target modules implicated in the interplay between rice and <i>Xanthomonas oryzae</i> pv. <i>oryzae</i> (<i>Xoo</i...
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| Autores principales: | , , , , , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
MDPI AG
2021
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/b3a4e07673a642b0b12045ad3c00dad3 |
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| Sumario: | MicroRNAs (miRNAs) handle immune response to pathogens by adjusting the function of target genes in plants. However, the experimentally documented miRNA/target modules implicated in the interplay between rice and <i>Xanthomonas oryzae</i> pv. <i>oryzae</i> (<i>Xoo</i>) are still in the early stages. Herein, the expression of osa-miR1432 was induced in resistant genotype IRBB5, but not susceptible genotype IR24, under <i>Xoo</i> strain PXO86 attack. Overexpressed osa-miR1432 heightened rice disease resistance to <i>Xoo</i>, indicated by enhancive enrichment of defense marker genes, raised reactive oxygen species (ROS) levels, repressed bacterial growth and shortened leaf lesion length, whilst the disruptive accumulation of osa-miR1432 accelerated rice susceptibility to <i>Xoo</i> infection. Noticeably, <i>OsCaML2</i> (<i>LOC_Os03g59770</i>) was experimentally confirmed as a target gene of osa-miR1432, and the overexpressing <i>OsCaML2</i> transgenic plants exhibited compromised resistance to <i>Xoo</i> infestation. Our results indicate that osa-miR1432 and <i>OsCaML2</i> were differently responsive to <i>Xoo</i> invasion at the transcriptional level and fine-tune rice resistance to <i>Xoo</i> infection, which may be referable in resistance gene discovery and valuable in the pursuit of improving <i>Xoo</i> resistance in rice breeding. |
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