Inducible Enrichment of Osa-miR1432 Confers Rice Bacterial Blight Resistance through Suppressing OsCaML2

MicroRNAs (miRNAs) handle immune response to pathogens by adjusting the function of target genes in plants. However, the experimentally documented miRNA/target modules implicated in the interplay between rice and Xanthomonas oryzae pv. oryzae (Xoo</i...

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Autores principales:	Yanfeng Jia, Quanlin Li, Yuying Li, Wenxue Zhai, Guanghuai Jiang, Chunrong Li
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	rice bacterial blight osa-miR1432 <i>OsCaML2</i> Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/b3a4e07673a642b0b12045ad3c00dad3
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spelling	oai:doaj.org-article:b3a4e07673a642b0b12045ad3c00dad32021-11-11T16:50:26ZInducible Enrichment of Osa-miR1432 Confers Rice Bacterial Blight Resistance through Suppressing <i>OsCaML2</i>10.3390/ijms2221113671422-00671661-6596https://doaj.org/article/b3a4e07673a642b0b12045ad3c00dad32021-10-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/11367https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067MicroRNAs (miRNAs) handle immune response to pathogens by adjusting the function of target genes in plants. However, the experimentally documented miRNA/target modules implicated in the interplay between rice and <i>Xanthomonas oryzae</i> pv. <i>oryzae</i> (<i>Xoo</i>) are still in the early stages. Herein, the expression of osa-miR1432 was induced in resistant genotype IRBB5, but not susceptible genotype IR24, under <i>Xoo</i> strain PXO86 attack. Overexpressed osa-miR1432 heightened rice disease resistance to <i>Xoo</i>, indicated by enhancive enrichment of defense marker genes, raised reactive oxygen species (ROS) levels, repressed bacterial growth and shortened leaf lesion length, whilst the disruptive accumulation of osa-miR1432 accelerated rice susceptibility to <i>Xoo</i> infection. Noticeably, <i>OsCaML2</i> (<i>LOC_Os03g59770</i>) was experimentally confirmed as a target gene of osa-miR1432, and the overexpressing <i>OsCaML2</i> transgenic plants exhibited compromised resistance to <i>Xoo</i> infestation. Our results indicate that osa-miR1432 and <i>OsCaML2</i> were differently responsive to <i>Xoo</i> invasion at the transcriptional level and fine-tune rice resistance to <i>Xoo</i> infection, which may be referable in resistance gene discovery and valuable in the pursuit of improving <i>Xoo</i> resistance in rice breeding.Yanfeng JiaQuanlin LiYuying LiWenxue ZhaiGuanghuai JiangChunrong LiMDPI AGarticlerice bacterial blightosa-miR1432<i>OsCaML2</i>Biology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 11367, p 11367 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	rice bacterial blight osa-miR1432 <i>OsCaML2</i> Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	rice bacterial blight osa-miR1432 <i>OsCaML2</i> Biology (General) QH301-705.5 Chemistry QD1-999 Yanfeng Jia Quanlin Li Yuying Li Wenxue Zhai Guanghuai Jiang Chunrong Li Inducible Enrichment of Osa-miR1432 Confers Rice Bacterial Blight Resistance through Suppressing <i>OsCaML2</i>
description	MicroRNAs (miRNAs) handle immune response to pathogens by adjusting the function of target genes in plants. However, the experimentally documented miRNA/target modules implicated in the interplay between rice and <i>Xanthomonas oryzae</i> pv. <i>oryzae</i> (<i>Xoo</i>) are still in the early stages. Herein, the expression of osa-miR1432 was induced in resistant genotype IRBB5, but not susceptible genotype IR24, under <i>Xoo</i> strain PXO86 attack. Overexpressed osa-miR1432 heightened rice disease resistance to <i>Xoo</i>, indicated by enhancive enrichment of defense marker genes, raised reactive oxygen species (ROS) levels, repressed bacterial growth and shortened leaf lesion length, whilst the disruptive accumulation of osa-miR1432 accelerated rice susceptibility to <i>Xoo</i> infection. Noticeably, <i>OsCaML2</i> (<i>LOC_Os03g59770</i>) was experimentally confirmed as a target gene of osa-miR1432, and the overexpressing <i>OsCaML2</i> transgenic plants exhibited compromised resistance to <i>Xoo</i> infestation. Our results indicate that osa-miR1432 and <i>OsCaML2</i> were differently responsive to <i>Xoo</i> invasion at the transcriptional level and fine-tune rice resistance to <i>Xoo</i> infection, which may be referable in resistance gene discovery and valuable in the pursuit of improving <i>Xoo</i> resistance in rice breeding.
format	article
author	Yanfeng Jia Quanlin Li Yuying Li Wenxue Zhai Guanghuai Jiang Chunrong Li
author_facet	Yanfeng Jia Quanlin Li Yuying Li Wenxue Zhai Guanghuai Jiang Chunrong Li
author_sort	Yanfeng Jia
title	Inducible Enrichment of Osa-miR1432 Confers Rice Bacterial Blight Resistance through Suppressing <i>OsCaML2</i>
title_short	Inducible Enrichment of Osa-miR1432 Confers Rice Bacterial Blight Resistance through Suppressing <i>OsCaML2</i>
title_full	Inducible Enrichment of Osa-miR1432 Confers Rice Bacterial Blight Resistance through Suppressing <i>OsCaML2</i>
title_fullStr	Inducible Enrichment of Osa-miR1432 Confers Rice Bacterial Blight Resistance through Suppressing <i>OsCaML2</i>
title_full_unstemmed	Inducible Enrichment of Osa-miR1432 Confers Rice Bacterial Blight Resistance through Suppressing <i>OsCaML2</i>
title_sort	inducible enrichment of osa-mir1432 confers rice bacterial blight resistance through suppressing <i>oscaml2</i>
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/b3a4e07673a642b0b12045ad3c00dad3
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Inducible Enrichment of Osa-miR1432 Confers Rice Bacterial Blight Resistance through Suppressing <i>OsCaML2</i>

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