Molecular characterization of clinical isolates of Aeromonas species from Malaysia.

<h4>Background</h4>Aeromonas species are common inhabitants of aquatic environments giving rise to infections in both fish and humans. Identification of aeromonads to the species level is problematic and complex due to their phenotypic and genotypic heterogeneity.<h4>Methodology/pr...

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Autores principales:	S D Puthucheary, Suat Moi Puah, Kek Heng Chua
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b3b2b120da2a474d855b7c2fa87c29fa
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spelling	oai:doaj.org-article:b3b2b120da2a474d855b7c2fa87c29fa2021-11-18T07:26:46ZMolecular characterization of clinical isolates of Aeromonas species from Malaysia.1932-620310.1371/journal.pone.0030205https://doaj.org/article/b3b2b120da2a474d855b7c2fa87c29fa2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22383958/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Aeromonas species are common inhabitants of aquatic environments giving rise to infections in both fish and humans. Identification of aeromonads to the species level is problematic and complex due to their phenotypic and genotypic heterogeneity.<h4>Methodology/principal findings</h4>Aeromonas hydrophila or Aeromonas sp were genetically re-identified using a combination of previously published methods targeting GCAT, 16S rDNA and rpoD genes. Characterization based on the genus specific GCAT-PCR showed that 94 (96%) of the 98 strains belonged to the genus Aeromonas. Considering the patterns obtained for the 94 isolates with the 16S rDNA-RFLP identification method, 3 clusters were recognised, i.e. A. caviae (61%), A. hydrophila (17%) and an unknown group (22%) with atypical RFLP restriction patterns. However, the phylogenetic tree constructed with the obtained rpoD sequences showed that 47 strains (50%) clustered with the sequence of the type strain of A. aquariorum, 18 (19%) with A. caviae, 16 (17%) with A. hydrophila, 12 (13%) with A. veronii and one strain (1%) with the type strain of A. trota. PCR investigation revealed the presence of 10 virulence genes in the 94 isolates as: lip (91%), exu (87%), ela (86%), alt (79%), ser (77%), fla (74%), aer (72%), act (43%), aexT (24%) and ast (23%).<h4>Conclusions/significance</h4>This study emphasizes the importance of using more than one method for the correct identification of Aeromonas strains. The sequences of the rpoD gene enabled the unambiguous identication of the 94 Aeromonas isolates in accordance with results of other recent studies. Aeromonas aquariorum showed to be the most prevalent species (50%) containing an important subset of virulence genes lip/alt/ser/fla/aer. Different combinations of the virulence genes present in the isolates indicate their probable role in the pathogenesis of Aeromonas infections.S D PuthuchearySuat Moi PuahKek Heng ChuaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 2, p e30205 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q S D Puthucheary Suat Moi Puah Kek Heng Chua Molecular characterization of clinical isolates of Aeromonas species from Malaysia.
description	<h4>Background</h4>Aeromonas species are common inhabitants of aquatic environments giving rise to infections in both fish and humans. Identification of aeromonads to the species level is problematic and complex due to their phenotypic and genotypic heterogeneity.<h4>Methodology/principal findings</h4>Aeromonas hydrophila or Aeromonas sp were genetically re-identified using a combination of previously published methods targeting GCAT, 16S rDNA and rpoD genes. Characterization based on the genus specific GCAT-PCR showed that 94 (96%) of the 98 strains belonged to the genus Aeromonas. Considering the patterns obtained for the 94 isolates with the 16S rDNA-RFLP identification method, 3 clusters were recognised, i.e. A. caviae (61%), A. hydrophila (17%) and an unknown group (22%) with atypical RFLP restriction patterns. However, the phylogenetic tree constructed with the obtained rpoD sequences showed that 47 strains (50%) clustered with the sequence of the type strain of A. aquariorum, 18 (19%) with A. caviae, 16 (17%) with A. hydrophila, 12 (13%) with A. veronii and one strain (1%) with the type strain of A. trota. PCR investigation revealed the presence of 10 virulence genes in the 94 isolates as: lip (91%), exu (87%), ela (86%), alt (79%), ser (77%), fla (74%), aer (72%), act (43%), aexT (24%) and ast (23%).<h4>Conclusions/significance</h4>This study emphasizes the importance of using more than one method for the correct identification of Aeromonas strains. The sequences of the rpoD gene enabled the unambiguous identication of the 94 Aeromonas isolates in accordance with results of other recent studies. Aeromonas aquariorum showed to be the most prevalent species (50%) containing an important subset of virulence genes lip/alt/ser/fla/aer. Different combinations of the virulence genes present in the isolates indicate their probable role in the pathogenesis of Aeromonas infections.
format	article
author	S D Puthucheary Suat Moi Puah Kek Heng Chua
author_facet	S D Puthucheary Suat Moi Puah Kek Heng Chua
author_sort	S D Puthucheary
title	Molecular characterization of clinical isolates of Aeromonas species from Malaysia.
title_short	Molecular characterization of clinical isolates of Aeromonas species from Malaysia.
title_full	Molecular characterization of clinical isolates of Aeromonas species from Malaysia.
title_fullStr	Molecular characterization of clinical isolates of Aeromonas species from Malaysia.
title_full_unstemmed	Molecular characterization of clinical isolates of Aeromonas species from Malaysia.
title_sort	molecular characterization of clinical isolates of aeromonas species from malaysia.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/b3b2b120da2a474d855b7c2fa87c29fa
work_keys_str_mv	AT sdputhucheary molecularcharacterizationofclinicalisolatesofaeromonasspeciesfrommalaysia AT suatmoipuah molecularcharacterizationofclinicalisolatesofaeromonasspeciesfrommalaysia AT kekhengchua molecularcharacterizationofclinicalisolatesofaeromonasspeciesfrommalaysia
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Molecular characterization of clinical isolates of Aeromonas species from Malaysia.

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