Live-cell imaging of circadian clock protein dynamics in CRISPR-generated knock-in cells

Live-cell imaging of circadian clock protein dynamics in CRISPR-generated knock-in cells

Live-cell recordings have been an important tool for studying circadian rhythms. Here the authors use CRISPR gene editing mediated knock-in to fluorescently tag Per2 and Cry1, and study cellular circadian dynamics of these two clock proteins.

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Detalles Bibliográficos
Autores principales: Christian H. Gabriel, Marta del Olmo, Amin Zehtabian, Marten Jäger, Silke Reischl, Hannah van Dijk, Carolin Ulbricht, Asylkhan Rakhymzhan, Thomas Korte, Barbara Koller, Astrid Grudziecki, Bert Maier, Andreas Herrmann, Raluca Niesner, Tomasz Zemojtel, Helge Ewers, Adrián E. Granada, Hanspeter Herzel, Achim Kramer
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2021
Materias:
Science
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Acceso en línea:https://doaj.org/article/b3b650cbf5af40ee9a15a843fab1524b
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Descripción
Sumario:Live-cell recordings have been an important tool for studying circadian rhythms. Here the authors use CRISPR gene editing mediated knock-in to fluorescently tag Per2 and Cry1, and study cellular circadian dynamics of these two clock proteins.

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