Live-cell imaging of circadian clock protein dynamics in CRISPR-generated knock-in cells
Live-cell recordings have been an important tool for studying circadian rhythms. Here the authors use CRISPR gene editing mediated knock-in to fluorescently tag Per2 and Cry1, and study cellular circadian dynamics of these two clock proteins.
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Nature Portfolio
2021
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oai:doaj.org-article:b3b650cbf5af40ee9a15a843fab1524b2021-12-02T16:04:16ZLive-cell imaging of circadian clock protein dynamics in CRISPR-generated knock-in cells10.1038/s41467-021-24086-92041-1723https://doaj.org/article/b3b650cbf5af40ee9a15a843fab1524b2021-06-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-24086-9https://doaj.org/toc/2041-1723Live-cell recordings have been an important tool for studying circadian rhythms. Here the authors use CRISPR gene editing mediated knock-in to fluorescently tag Per2 and Cry1, and study cellular circadian dynamics of these two clock proteins.Christian H. GabrielMarta del OlmoAmin ZehtabianMarten JägerSilke ReischlHannah van DijkCarolin UlbrichtAsylkhan RakhymzhanThomas KorteBarbara KollerAstrid GrudzieckiBert MaierAndreas HerrmannRaluca NiesnerTomasz ZemojtelHelge EwersAdrián E. GranadaHanspeter HerzelAchim KramerNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-15 (2021) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Science Q |
| spellingShingle |
Science Q Christian H. Gabriel Marta del Olmo Amin Zehtabian Marten Jäger Silke Reischl Hannah van Dijk Carolin Ulbricht Asylkhan Rakhymzhan Thomas Korte Barbara Koller Astrid Grudziecki Bert Maier Andreas Herrmann Raluca Niesner Tomasz Zemojtel Helge Ewers Adrián E. Granada Hanspeter Herzel Achim Kramer Live-cell imaging of circadian clock protein dynamics in CRISPR-generated knock-in cells |
| description |
Live-cell recordings have been an important tool for studying circadian rhythms. Here the authors use CRISPR gene editing mediated knock-in to fluorescently tag Per2 and Cry1, and study cellular circadian dynamics of these two clock proteins. |
| format |
article |
| author |
Christian H. Gabriel Marta del Olmo Amin Zehtabian Marten Jäger Silke Reischl Hannah van Dijk Carolin Ulbricht Asylkhan Rakhymzhan Thomas Korte Barbara Koller Astrid Grudziecki Bert Maier Andreas Herrmann Raluca Niesner Tomasz Zemojtel Helge Ewers Adrián E. Granada Hanspeter Herzel Achim Kramer |
| author_facet |
Christian H. Gabriel Marta del Olmo Amin Zehtabian Marten Jäger Silke Reischl Hannah van Dijk Carolin Ulbricht Asylkhan Rakhymzhan Thomas Korte Barbara Koller Astrid Grudziecki Bert Maier Andreas Herrmann Raluca Niesner Tomasz Zemojtel Helge Ewers Adrián E. Granada Hanspeter Herzel Achim Kramer |
| author_sort |
Christian H. Gabriel |
| title |
Live-cell imaging of circadian clock protein dynamics in CRISPR-generated knock-in cells |
| title_short |
Live-cell imaging of circadian clock protein dynamics in CRISPR-generated knock-in cells |
| title_full |
Live-cell imaging of circadian clock protein dynamics in CRISPR-generated knock-in cells |
| title_fullStr |
Live-cell imaging of circadian clock protein dynamics in CRISPR-generated knock-in cells |
| title_full_unstemmed |
Live-cell imaging of circadian clock protein dynamics in CRISPR-generated knock-in cells |
| title_sort |
live-cell imaging of circadian clock protein dynamics in crispr-generated knock-in cells |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/b3b650cbf5af40ee9a15a843fab1524b |
| work_keys_str_mv |
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