Plasma concentrations of branched-chain amino acids differ with Holstein genetic strain in pasture-based dairy systems

Abstract In pasture-based systems, there are nutritional and climatic challenges exacerbated across lactation; thus, dairy cows require an enhanced adaptive capacity compared with cows in confined systems. We aimed to evaluate the effect of lactation stage (21 vs. 180 days in milk, DIM) and Holstein...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ezequiel Jorge-Smeding, Mariana Carriquiry, Gonzalo Cantalapiedra-Hijar, Alejandro Mendoza, Ana Laura Astessiano
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b3bc1aee6d04446ca8f0fb783e0c58eb
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:b3bc1aee6d04446ca8f0fb783e0c58eb
record_format	dspace
spelling	oai:doaj.org-article:b3bc1aee6d04446ca8f0fb783e0c58eb2021-11-21T12:21:19ZPlasma concentrations of branched-chain amino acids differ with Holstein genetic strain in pasture-based dairy systems10.1038/s41598-021-01564-02045-2322https://doaj.org/article/b3bc1aee6d04446ca8f0fb783e0c58eb2021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-01564-0https://doaj.org/toc/2045-2322Abstract In pasture-based systems, there are nutritional and climatic challenges exacerbated across lactation; thus, dairy cows require an enhanced adaptive capacity compared with cows in confined systems. We aimed to evaluate the effect of lactation stage (21 vs. 180 days in milk, DIM) and Holstein genetic strain (North American Holstein, NAH, n = 8; New Zealand Holstein, NZH, n = 8) on metabolic adaptations of grazing dairy cows through plasma metabolomic profiling and its association with classical metabolites. Although 67 metabolites were affected (FDR < 0.05) by DIM, no metabolite was observed to differ between genetic strains while only alanine was affected (FDR = 0.02) by the interaction between genetic strain and DIM. However, complementary tools for time-series analysis (ASCA analysis, MEBA ranking) indicated that alanine and the branched-chain amino acids (BCAA) differed between genetic strains in a lactation-stage dependent manner. Indeed, NZH cows had lower (P-Tukey < 0.05) plasma concentrations of leucine, isoleucine and valine than NAH cows at 21 DIM, probably signaling for greater insulin sensitivity. Metabolic pathway analysis also revealed that, independently of genetic strains, AA metabolism might be structurally involved in homeorhetic changes as 40% (19/46) of metabolic pathways differentially expressed (FDR < 0.05) between 21 and 180 DIM belonged to AA metabolism.Ezequiel Jorge-SmedingMariana CarriquiryGonzalo Cantalapiedra-HijarAlejandro MendozaAna Laura AstessianoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ezequiel Jorge-Smeding Mariana Carriquiry Gonzalo Cantalapiedra-Hijar Alejandro Mendoza Ana Laura Astessiano Plasma concentrations of branched-chain amino acids differ with Holstein genetic strain in pasture-based dairy systems
description	Abstract In pasture-based systems, there are nutritional and climatic challenges exacerbated across lactation; thus, dairy cows require an enhanced adaptive capacity compared with cows in confined systems. We aimed to evaluate the effect of lactation stage (21 vs. 180 days in milk, DIM) and Holstein genetic strain (North American Holstein, NAH, n = 8; New Zealand Holstein, NZH, n = 8) on metabolic adaptations of grazing dairy cows through plasma metabolomic profiling and its association with classical metabolites. Although 67 metabolites were affected (FDR < 0.05) by DIM, no metabolite was observed to differ between genetic strains while only alanine was affected (FDR = 0.02) by the interaction between genetic strain and DIM. However, complementary tools for time-series analysis (ASCA analysis, MEBA ranking) indicated that alanine and the branched-chain amino acids (BCAA) differed between genetic strains in a lactation-stage dependent manner. Indeed, NZH cows had lower (P-Tukey < 0.05) plasma concentrations of leucine, isoleucine and valine than NAH cows at 21 DIM, probably signaling for greater insulin sensitivity. Metabolic pathway analysis also revealed that, independently of genetic strains, AA metabolism might be structurally involved in homeorhetic changes as 40% (19/46) of metabolic pathways differentially expressed (FDR < 0.05) between 21 and 180 DIM belonged to AA metabolism.
format	article
author	Ezequiel Jorge-Smeding Mariana Carriquiry Gonzalo Cantalapiedra-Hijar Alejandro Mendoza Ana Laura Astessiano
author_facet	Ezequiel Jorge-Smeding Mariana Carriquiry Gonzalo Cantalapiedra-Hijar Alejandro Mendoza Ana Laura Astessiano
author_sort	Ezequiel Jorge-Smeding
title	Plasma concentrations of branched-chain amino acids differ with Holstein genetic strain in pasture-based dairy systems
title_short	Plasma concentrations of branched-chain amino acids differ with Holstein genetic strain in pasture-based dairy systems
title_full	Plasma concentrations of branched-chain amino acids differ with Holstein genetic strain in pasture-based dairy systems
title_fullStr	Plasma concentrations of branched-chain amino acids differ with Holstein genetic strain in pasture-based dairy systems
title_full_unstemmed	Plasma concentrations of branched-chain amino acids differ with Holstein genetic strain in pasture-based dairy systems
title_sort	plasma concentrations of branched-chain amino acids differ with holstein genetic strain in pasture-based dairy systems
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/b3bc1aee6d04446ca8f0fb783e0c58eb
work_keys_str_mv	AT ezequieljorgesmeding plasmaconcentrationsofbranchedchainaminoacidsdifferwithholsteingeneticstraininpasturebaseddairysystems AT marianacarriquiry plasmaconcentrationsofbranchedchainaminoacidsdifferwithholsteingeneticstraininpasturebaseddairysystems AT gonzalocantalapiedrahijar plasmaconcentrationsofbranchedchainaminoacidsdifferwithholsteingeneticstraininpasturebaseddairysystems AT alejandromendoza plasmaconcentrationsofbranchedchainaminoacidsdifferwithholsteingeneticstraininpasturebaseddairysystems AT analauraastessiano plasmaconcentrationsofbranchedchainaminoacidsdifferwithholsteingeneticstraininpasturebaseddairysystems
_version_	1718419088624058368

Plasma concentrations of branched-chain amino acids differ with Holstein genetic strain in pasture-based dairy systems

Ejemplares similares