Tibial dyschondroplasia is highly associated with suppression of tibial angiogenesis through regulating the HIF-1α/VEGF/VEGFR signaling pathway in chickens

Abstract Tibial dyschondroplasia (TD) is an intractable poultry problem that is characterized by the appearance of non-vascularized and non-mineralized cartilage masses in tibial growth plates (TGPs). However, the role of angiogenesis inhibition in the occurrence of TD remains unknown. In this study...

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Autores principales:	Shu-cheng Huang, Mujeeb Ur Rehman, Yan-fang Lan, Gang Qiu, Hui Zhang, Muhammad Kashif Iqbal, Hou-qiang Luo, Khalid Mehmood, Li-hong Zhang, Jia-kui Li
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b3be457428c546d0a3a67d70cefaa094
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spelling	oai:doaj.org-article:b3be457428c546d0a3a67d70cefaa0942021-12-02T16:06:44ZTibial dyschondroplasia is highly associated with suppression of tibial angiogenesis through regulating the HIF-1α/VEGF/VEGFR signaling pathway in chickens10.1038/s41598-017-09664-62045-2322https://doaj.org/article/b3be457428c546d0a3a67d70cefaa0942017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-09664-6https://doaj.org/toc/2045-2322Abstract Tibial dyschondroplasia (TD) is an intractable poultry problem that is characterized by the appearance of non-vascularized and non-mineralized cartilage masses in tibial growth plates (TGPs). However, the role of angiogenesis inhibition in the occurrence of TD remains unknown. In this study, we found that, compared to low-altitude Arbor Acres chickens (AACs), high-altitude Tibetan chickens showed higher tibial vascular distributions that were accompanied by up-regulation of hypoxia-inducible factor-1α (HIF-1α), vascular endothelial growth factor A (VEGFA) and VEGF receptors. These observations provide insights into hypoxia-induced angiogenesis, which may be related to the absence of TD in high-altitude native Tibetan chickens. Importantly, hypoxia experiments also showed that during hypoxia, tibial angiogenesis was enhanced, which was due to pro-angiogenic factor up-regulation (including VEGFA, VEGFR1, VEGFR2, and IL-8), in AACs. Moreover, we observed that thiram-induced TD could strongly inhibit tibial angiogenesis in the hypertrophic zone through coordinated down-regulation of HIF-1α and pro-angiogenic factors, leading to a disruption in the blood supply to the TGP. Taken together, these findings reveal that the occurrence of TD is highly associated with inhibition of tibial angiogenesis through down-regulated expression of HIF-1α, VEGFA and VEGF receptors, which results in suppression of TGP development.Shu-cheng HuangMujeeb Ur RehmanYan-fang LanGang QiuHui ZhangMuhammad Kashif IqbalHou-qiang LuoKhalid MehmoodLi-hong ZhangJia-kui LiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-15 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shu-cheng Huang Mujeeb Ur Rehman Yan-fang Lan Gang Qiu Hui Zhang Muhammad Kashif Iqbal Hou-qiang Luo Khalid Mehmood Li-hong Zhang Jia-kui Li Tibial dyschondroplasia is highly associated with suppression of tibial angiogenesis through regulating the HIF-1α/VEGF/VEGFR signaling pathway in chickens
description	Abstract Tibial dyschondroplasia (TD) is an intractable poultry problem that is characterized by the appearance of non-vascularized and non-mineralized cartilage masses in tibial growth plates (TGPs). However, the role of angiogenesis inhibition in the occurrence of TD remains unknown. In this study, we found that, compared to low-altitude Arbor Acres chickens (AACs), high-altitude Tibetan chickens showed higher tibial vascular distributions that were accompanied by up-regulation of hypoxia-inducible factor-1α (HIF-1α), vascular endothelial growth factor A (VEGFA) and VEGF receptors. These observations provide insights into hypoxia-induced angiogenesis, which may be related to the absence of TD in high-altitude native Tibetan chickens. Importantly, hypoxia experiments also showed that during hypoxia, tibial angiogenesis was enhanced, which was due to pro-angiogenic factor up-regulation (including VEGFA, VEGFR1, VEGFR2, and IL-8), in AACs. Moreover, we observed that thiram-induced TD could strongly inhibit tibial angiogenesis in the hypertrophic zone through coordinated down-regulation of HIF-1α and pro-angiogenic factors, leading to a disruption in the blood supply to the TGP. Taken together, these findings reveal that the occurrence of TD is highly associated with inhibition of tibial angiogenesis through down-regulated expression of HIF-1α, VEGFA and VEGF receptors, which results in suppression of TGP development.
format	article
author	Shu-cheng Huang Mujeeb Ur Rehman Yan-fang Lan Gang Qiu Hui Zhang Muhammad Kashif Iqbal Hou-qiang Luo Khalid Mehmood Li-hong Zhang Jia-kui Li
author_facet	Shu-cheng Huang Mujeeb Ur Rehman Yan-fang Lan Gang Qiu Hui Zhang Muhammad Kashif Iqbal Hou-qiang Luo Khalid Mehmood Li-hong Zhang Jia-kui Li
author_sort	Shu-cheng Huang
title	Tibial dyschondroplasia is highly associated with suppression of tibial angiogenesis through regulating the HIF-1α/VEGF/VEGFR signaling pathway in chickens
title_short	Tibial dyschondroplasia is highly associated with suppression of tibial angiogenesis through regulating the HIF-1α/VEGF/VEGFR signaling pathway in chickens
title_full	Tibial dyschondroplasia is highly associated with suppression of tibial angiogenesis through regulating the HIF-1α/VEGF/VEGFR signaling pathway in chickens
title_fullStr	Tibial dyschondroplasia is highly associated with suppression of tibial angiogenesis through regulating the HIF-1α/VEGF/VEGFR signaling pathway in chickens
title_full_unstemmed	Tibial dyschondroplasia is highly associated with suppression of tibial angiogenesis through regulating the HIF-1α/VEGF/VEGFR signaling pathway in chickens
title_sort	tibial dyschondroplasia is highly associated with suppression of tibial angiogenesis through regulating the hif-1α/vegf/vegfr signaling pathway in chickens
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/b3be457428c546d0a3a67d70cefaa094
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Tibial dyschondroplasia is highly associated with suppression of tibial angiogenesis through regulating the HIF-1α/VEGF/VEGFR signaling pathway in chickens

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