Patch-seq of mouse DRG neurons reveals candidate genes for specific mechanosensory functions

Summary: A variety of mechanosensory neurons are involved in touch, proprioception, and pain. Many molecular components of the mechanotransduction machinery subserving these sensory modalities remain to be discovered. Here, we combine recordings of mechanosensitive (MS) currents in mechanosensory ne...

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Autores principales:	Thibaud Parpaite, Lucie Brosse, Nina Séjourné, Amandine Laur, Yasmine Mechioukhi, Patrick Delmas, Bertrand Coste
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	somatosensation mechanotransduction nociception ion channel pain sensory neuron Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/b3c178cbdcb84202b05eb60b4926d9f0
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spelling	oai:doaj.org-article:b3c178cbdcb84202b05eb60b4926d9f02021-11-04T04:29:01ZPatch-seq of mouse DRG neurons reveals candidate genes for specific mechanosensory functions2211-124710.1016/j.celrep.2021.109914https://doaj.org/article/b3c178cbdcb84202b05eb60b4926d9f02021-11-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2211124721013875https://doaj.org/toc/2211-1247Summary: A variety of mechanosensory neurons are involved in touch, proprioception, and pain. Many molecular components of the mechanotransduction machinery subserving these sensory modalities remain to be discovered. Here, we combine recordings of mechanosensitive (MS) currents in mechanosensory neurons with single-cell RNA sequencing. Transcriptional profiles are mapped onto previously identified sensory neuron types to identify cell-type correlates between datasets. Correlation of current signatures with single-cell transcriptomes provides a one-to-one correspondence between mechanoelectric properties and transcriptomically defined neuronal populations. Moreover, a gene-expression differential comparison provides a set of candidate genes for mechanotransduction complexes. Piezo2 is expectedly found to be enriched in rapidly adapting MS current-expressing neurons, whereas Tmem120a and Tmem150c, thought to mediate slow-type MS currents, are uniformly expressed in all mechanosensory neuron subtypes. Further knockdown experiments disqualify them as mediating MS currents in sensory neurons. This dataset constitutes an open resource to explore further the cell-type-specific determinants of mechanosensory properties.Thibaud ParpaiteLucie BrosseNina SéjournéAmandine LaurYasmine MechioukhiPatrick DelmasBertrand CosteElsevierarticlesomatosensationmechanotransductionnociceptionion channelpainsensory neuronBiology (General)QH301-705.5ENCell Reports, Vol 37, Iss 5, Pp 109914- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	somatosensation mechanotransduction nociception ion channel pain sensory neuron Biology (General) QH301-705.5
spellingShingle	somatosensation mechanotransduction nociception ion channel pain sensory neuron Biology (General) QH301-705.5 Thibaud Parpaite Lucie Brosse Nina Séjourné Amandine Laur Yasmine Mechioukhi Patrick Delmas Bertrand Coste Patch-seq of mouse DRG neurons reveals candidate genes for specific mechanosensory functions
description	Summary: A variety of mechanosensory neurons are involved in touch, proprioception, and pain. Many molecular components of the mechanotransduction machinery subserving these sensory modalities remain to be discovered. Here, we combine recordings of mechanosensitive (MS) currents in mechanosensory neurons with single-cell RNA sequencing. Transcriptional profiles are mapped onto previously identified sensory neuron types to identify cell-type correlates between datasets. Correlation of current signatures with single-cell transcriptomes provides a one-to-one correspondence between mechanoelectric properties and transcriptomically defined neuronal populations. Moreover, a gene-expression differential comparison provides a set of candidate genes for mechanotransduction complexes. Piezo2 is expectedly found to be enriched in rapidly adapting MS current-expressing neurons, whereas Tmem120a and Tmem150c, thought to mediate slow-type MS currents, are uniformly expressed in all mechanosensory neuron subtypes. Further knockdown experiments disqualify them as mediating MS currents in sensory neurons. This dataset constitutes an open resource to explore further the cell-type-specific determinants of mechanosensory properties.
format	article
author	Thibaud Parpaite Lucie Brosse Nina Séjourné Amandine Laur Yasmine Mechioukhi Patrick Delmas Bertrand Coste
author_facet	Thibaud Parpaite Lucie Brosse Nina Séjourné Amandine Laur Yasmine Mechioukhi Patrick Delmas Bertrand Coste
author_sort	Thibaud Parpaite
title	Patch-seq of mouse DRG neurons reveals candidate genes for specific mechanosensory functions
title_short	Patch-seq of mouse DRG neurons reveals candidate genes for specific mechanosensory functions
title_full	Patch-seq of mouse DRG neurons reveals candidate genes for specific mechanosensory functions
title_fullStr	Patch-seq of mouse DRG neurons reveals candidate genes for specific mechanosensory functions
title_full_unstemmed	Patch-seq of mouse DRG neurons reveals candidate genes for specific mechanosensory functions
title_sort	patch-seq of mouse drg neurons reveals candidate genes for specific mechanosensory functions
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/b3c178cbdcb84202b05eb60b4926d9f0
work_keys_str_mv	AT thibaudparpaite patchseqofmousedrgneuronsrevealscandidategenesforspecificmechanosensoryfunctions AT luciebrosse patchseqofmousedrgneuronsrevealscandidategenesforspecificmechanosensoryfunctions AT ninasejourne patchseqofmousedrgneuronsrevealscandidategenesforspecificmechanosensoryfunctions AT amandinelaur patchseqofmousedrgneuronsrevealscandidategenesforspecificmechanosensoryfunctions AT yasminemechioukhi patchseqofmousedrgneuronsrevealscandidategenesforspecificmechanosensoryfunctions AT patrickdelmas patchseqofmousedrgneuronsrevealscandidategenesforspecificmechanosensoryfunctions AT bertrandcoste patchseqofmousedrgneuronsrevealscandidategenesforspecificmechanosensoryfunctions
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Patch-seq of mouse DRG neurons reveals candidate genes for specific mechanosensory functions

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