Urinary proteomics investigations into contrast-induced acute kidney injury.

Some patients have a decline in renal function after contrast medium injection, and this phenomenon is called contrast-induced acute kidney injury (CI-AKI); a small number of people even suffer severe renal failure. To date, the mechanism of CI-AKI remains unclear. We aimed to identify novel potenti...

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Autores principales:	Hong Zhu, Wenwen Chu, Shuai Han, Bihu Gao, Xin Wang
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b3c4f68498c14653bee39c661f2c7a9c
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spelling	oai:doaj.org-article:b3c4f68498c14653bee39c661f2c7a9c2021-12-02T20:16:44ZUrinary proteomics investigations into contrast-induced acute kidney injury.1932-620310.1371/journal.pone.0258736https://doaj.org/article/b3c4f68498c14653bee39c661f2c7a9c2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0258736https://doaj.org/toc/1932-6203Some patients have a decline in renal function after contrast medium injection, and this phenomenon is called contrast-induced acute kidney injury (CI-AKI); a small number of people even suffer severe renal failure. To date, the mechanism of CI-AKI remains unclear. We aimed to identify novel potential biomarkers in the urine of patients with CI-AKI through LC-MS/MS and bioinformatics analysis. We enrolled patients who underwent coronary angiography (contrast agent: iohexol). The CI-AKI group included 4 cases, and the non-CI-AKI group included 20 cases. We mixed the 4 CI-AKI samples and 20 non-CI-AKI samples. Then, a 0.6 ml urine sample was used for proteome analysis with LC-MS/MS approach. Metascape, ExPASy, and the Human Protein Atlas were utilized for bioinformatics analysis. We obtained 724 and 830 urine proteins from the CI-AKI and non-CI-AKI groups, respectively. The distribution of the pI values and molecular weights (MWs) of postoperative urine proteins showed no significant difference between the CI-AKI group and the non-CI-AKI group. A total of 99differentially expressed proteins (DEPs) were detected, among which 18 proteins were detected only in tubule cells, and 19 proteins were detected in both tubule cells and glomeruli. With GO analysis, the GEPs were mainly associated with immune response and inflammation. Although biomarkers cannot be asserted from this single pilot study, our results may help advance the understanding of the mechanisms of CI-AKI and identify potential novel biomarkers for further investigation.Hong ZhuWenwen ChuShuai HanBihu GaoXin WangPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 10, p e0258736 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hong Zhu Wenwen Chu Shuai Han Bihu Gao Xin Wang Urinary proteomics investigations into contrast-induced acute kidney injury.
description	Some patients have a decline in renal function after contrast medium injection, and this phenomenon is called contrast-induced acute kidney injury (CI-AKI); a small number of people even suffer severe renal failure. To date, the mechanism of CI-AKI remains unclear. We aimed to identify novel potential biomarkers in the urine of patients with CI-AKI through LC-MS/MS and bioinformatics analysis. We enrolled patients who underwent coronary angiography (contrast agent: iohexol). The CI-AKI group included 4 cases, and the non-CI-AKI group included 20 cases. We mixed the 4 CI-AKI samples and 20 non-CI-AKI samples. Then, a 0.6 ml urine sample was used for proteome analysis with LC-MS/MS approach. Metascape, ExPASy, and the Human Protein Atlas were utilized for bioinformatics analysis. We obtained 724 and 830 urine proteins from the CI-AKI and non-CI-AKI groups, respectively. The distribution of the pI values and molecular weights (MWs) of postoperative urine proteins showed no significant difference between the CI-AKI group and the non-CI-AKI group. A total of 99differentially expressed proteins (DEPs) were detected, among which 18 proteins were detected only in tubule cells, and 19 proteins were detected in both tubule cells and glomeruli. With GO analysis, the GEPs were mainly associated with immune response and inflammation. Although biomarkers cannot be asserted from this single pilot study, our results may help advance the understanding of the mechanisms of CI-AKI and identify potential novel biomarkers for further investigation.
format	article
author	Hong Zhu Wenwen Chu Shuai Han Bihu Gao Xin Wang
author_facet	Hong Zhu Wenwen Chu Shuai Han Bihu Gao Xin Wang
author_sort	Hong Zhu
title	Urinary proteomics investigations into contrast-induced acute kidney injury.
title_short	Urinary proteomics investigations into contrast-induced acute kidney injury.
title_full	Urinary proteomics investigations into contrast-induced acute kidney injury.
title_fullStr	Urinary proteomics investigations into contrast-induced acute kidney injury.
title_full_unstemmed	Urinary proteomics investigations into contrast-induced acute kidney injury.
title_sort	urinary proteomics investigations into contrast-induced acute kidney injury.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/b3c4f68498c14653bee39c661f2c7a9c
work_keys_str_mv	AT hongzhu urinaryproteomicsinvestigationsintocontrastinducedacutekidneyinjury AT wenwenchu urinaryproteomicsinvestigationsintocontrastinducedacutekidneyinjury AT shuaihan urinaryproteomicsinvestigationsintocontrastinducedacutekidneyinjury AT bihugao urinaryproteomicsinvestigationsintocontrastinducedacutekidneyinjury AT xinwang urinaryproteomicsinvestigationsintocontrastinducedacutekidneyinjury
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Urinary proteomics investigations into contrast-induced acute kidney injury.

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