Single-cell RNA sequencing reveals differential cell cycle activity in key cell populations during nephrogenesis

Abstract The kidney is a complex organ composed of more than 30 terminally differentiated cell types that all are required to perform its numerous homeostatic functions. Defects in kidney development are a significant cause of chronic kidney disease in children, which can lead to kidney failure that...

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Autores principales:	Abha S. Bais, Débora M. Cerqueira, Andrew Clugston, Andrew J. Bodnar, Jacqueline Ho, Dennis Kostka
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b3c5a48e498b4cd986b8808059d58e0d
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spelling	oai:doaj.org-article:b3c5a48e498b4cd986b8808059d58e0d2021-11-21T12:24:04ZSingle-cell RNA sequencing reveals differential cell cycle activity in key cell populations during nephrogenesis10.1038/s41598-021-01790-62045-2322https://doaj.org/article/b3c5a48e498b4cd986b8808059d58e0d2021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-01790-6https://doaj.org/toc/2045-2322Abstract The kidney is a complex organ composed of more than 30 terminally differentiated cell types that all are required to perform its numerous homeostatic functions. Defects in kidney development are a significant cause of chronic kidney disease in children, which can lead to kidney failure that can only be treated by transplant or dialysis. A better understanding of molecular mechanisms that drive kidney development is important for designing strategies to enhance renal repair and regeneration. In this study, we profiled gene expression in the developing mouse kidney at embryonic day 14.5 at single-cell resolution. Consistent with previous studies, clusters with distinct transcriptional signatures clearly identify major compartments and cell types of the developing kidney. Cell cycle activity distinguishes between the “primed” and “self-renewing” sub-populations of nephron progenitors, with increased expression of the cell cycle-related genes Birc5, Cdca3, Smc2 and Smc4 in “primed” nephron progenitors. In addition, augmented expression of cell cycle related genes Birc5, Cks2, Ccnb1, Ccnd1 and Tuba1a/b was detected in immature distal tubules, suggesting cell cycle regulation may be required for early events of nephron patterning and tubular fusion between the distal nephron and collecting duct epithelia.Abha S. BaisDébora M. CerqueiraAndrew ClugstonAndrew J. BodnarJacqueline HoDennis KostkaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Abha S. Bais Débora M. Cerqueira Andrew Clugston Andrew J. Bodnar Jacqueline Ho Dennis Kostka Single-cell RNA sequencing reveals differential cell cycle activity in key cell populations during nephrogenesis
description	Abstract The kidney is a complex organ composed of more than 30 terminally differentiated cell types that all are required to perform its numerous homeostatic functions. Defects in kidney development are a significant cause of chronic kidney disease in children, which can lead to kidney failure that can only be treated by transplant or dialysis. A better understanding of molecular mechanisms that drive kidney development is important for designing strategies to enhance renal repair and regeneration. In this study, we profiled gene expression in the developing mouse kidney at embryonic day 14.5 at single-cell resolution. Consistent with previous studies, clusters with distinct transcriptional signatures clearly identify major compartments and cell types of the developing kidney. Cell cycle activity distinguishes between the “primed” and “self-renewing” sub-populations of nephron progenitors, with increased expression of the cell cycle-related genes Birc5, Cdca3, Smc2 and Smc4 in “primed” nephron progenitors. In addition, augmented expression of cell cycle related genes Birc5, Cks2, Ccnb1, Ccnd1 and Tuba1a/b was detected in immature distal tubules, suggesting cell cycle regulation may be required for early events of nephron patterning and tubular fusion between the distal nephron and collecting duct epithelia.
format	article
author	Abha S. Bais Débora M. Cerqueira Andrew Clugston Andrew J. Bodnar Jacqueline Ho Dennis Kostka
author_facet	Abha S. Bais Débora M. Cerqueira Andrew Clugston Andrew J. Bodnar Jacqueline Ho Dennis Kostka
author_sort	Abha S. Bais
title	Single-cell RNA sequencing reveals differential cell cycle activity in key cell populations during nephrogenesis
title_short	Single-cell RNA sequencing reveals differential cell cycle activity in key cell populations during nephrogenesis
title_full	Single-cell RNA sequencing reveals differential cell cycle activity in key cell populations during nephrogenesis
title_fullStr	Single-cell RNA sequencing reveals differential cell cycle activity in key cell populations during nephrogenesis
title_full_unstemmed	Single-cell RNA sequencing reveals differential cell cycle activity in key cell populations during nephrogenesis
title_sort	single-cell rna sequencing reveals differential cell cycle activity in key cell populations during nephrogenesis
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/b3c5a48e498b4cd986b8808059d58e0d
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Single-cell RNA sequencing reveals differential cell cycle activity in key cell populations during nephrogenesis

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