φBO1E, a newly discovered lytic bacteriophage targeting carbapenemase-producing Klebsiella pneumoniae of the pandemic Clonal Group 258 clade II lineage

Abstract The pandemic dissemination of KPC carbapenemase-producing Klebsiella pneumoniae (KPC-KP) represents a major public health problem, given their extensive multidrug resistance profiles and primary role in causing healthcare-associated infections. This phenomenon has largely been contributed b...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Marco Maria D’Andrea, Pasquale Marmo, Lucia Henrici De Angelis, Mattia Palmieri, Nagaia Ciacci, Gustavo Di Lallo, Elisa Demattè, Elisa Vannuccini, Pietro Lupetti, Gian Maria Rossolini, Maria Cristina Thaller
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b3c5fb4bb510433088f5f3221a3b3021
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:b3c5fb4bb510433088f5f3221a3b3021
record_format	dspace
spelling	oai:doaj.org-article:b3c5fb4bb510433088f5f3221a3b30212021-12-02T12:32:12ZφBO1E, a newly discovered lytic bacteriophage targeting carbapenemase-producing Klebsiella pneumoniae of the pandemic Clonal Group 258 clade II lineage10.1038/s41598-017-02788-92045-2322https://doaj.org/article/b3c5fb4bb510433088f5f3221a3b30212017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02788-9https://doaj.org/toc/2045-2322Abstract The pandemic dissemination of KPC carbapenemase-producing Klebsiella pneumoniae (KPC-KP) represents a major public health problem, given their extensive multidrug resistance profiles and primary role in causing healthcare-associated infections. This phenomenon has largely been contributed by strains of Clonal Group (CG) 258, mostly of clade II, which in some areas represent the majority of KPC-KP isolates. Here we have characterized a newly discovered lytic Podoviridae, named φBO1E, targeting KPC-KP strains of clade II lineage of CG258. Genomic sequencing revealed that φBO1E belongs to the Kp34virus genus (87% nucleotide identity to vB_KpnP_SU552A). ΦBO1E was stable over a broad pH and temperature range, exhibited strict specificity for K. pneumoniae strains of clade II of CG258, and was unable to establish lysogeny. In a Galleria mellonella infection model, φBO1E was able to protect larvae from death following infection with KPC-KP strains of clade II of CG258, including one colistin resistant strain characterized by a hypermucoviscous phenotype. To our best knowledge φBO1E is the first characterized lytic phage targeting K. pneumoniae strains of this pandemic clonal lineage. As such, it could be of potential interest to develop new agents for treatment of KPC-KP infections and for decolonization of subjects chronically colonized by these resistant superbugs.Marco Maria D’AndreaPasquale MarmoLucia Henrici De AngelisMattia PalmieriNagaia CiacciGustavo Di LalloElisa DemattèElisa VannucciniPietro LupettiGian Maria RossoliniMaria Cristina ThallerNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-8 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Marco Maria D’Andrea Pasquale Marmo Lucia Henrici De Angelis Mattia Palmieri Nagaia Ciacci Gustavo Di Lallo Elisa Demattè Elisa Vannuccini Pietro Lupetti Gian Maria Rossolini Maria Cristina Thaller φBO1E, a newly discovered lytic bacteriophage targeting carbapenemase-producing Klebsiella pneumoniae of the pandemic Clonal Group 258 clade II lineage
description	Abstract The pandemic dissemination of KPC carbapenemase-producing Klebsiella pneumoniae (KPC-KP) represents a major public health problem, given their extensive multidrug resistance profiles and primary role in causing healthcare-associated infections. This phenomenon has largely been contributed by strains of Clonal Group (CG) 258, mostly of clade II, which in some areas represent the majority of KPC-KP isolates. Here we have characterized a newly discovered lytic Podoviridae, named φBO1E, targeting KPC-KP strains of clade II lineage of CG258. Genomic sequencing revealed that φBO1E belongs to the Kp34virus genus (87% nucleotide identity to vB_KpnP_SU552A). ΦBO1E was stable over a broad pH and temperature range, exhibited strict specificity for K. pneumoniae strains of clade II of CG258, and was unable to establish lysogeny. In a Galleria mellonella infection model, φBO1E was able to protect larvae from death following infection with KPC-KP strains of clade II of CG258, including one colistin resistant strain characterized by a hypermucoviscous phenotype. To our best knowledge φBO1E is the first characterized lytic phage targeting K. pneumoniae strains of this pandemic clonal lineage. As such, it could be of potential interest to develop new agents for treatment of KPC-KP infections and for decolonization of subjects chronically colonized by these resistant superbugs.
format	article
author	Marco Maria D’Andrea Pasquale Marmo Lucia Henrici De Angelis Mattia Palmieri Nagaia Ciacci Gustavo Di Lallo Elisa Demattè Elisa Vannuccini Pietro Lupetti Gian Maria Rossolini Maria Cristina Thaller
author_facet	Marco Maria D’Andrea Pasquale Marmo Lucia Henrici De Angelis Mattia Palmieri Nagaia Ciacci Gustavo Di Lallo Elisa Demattè Elisa Vannuccini Pietro Lupetti Gian Maria Rossolini Maria Cristina Thaller
author_sort	Marco Maria D’Andrea
title	φBO1E, a newly discovered lytic bacteriophage targeting carbapenemase-producing Klebsiella pneumoniae of the pandemic Clonal Group 258 clade II lineage
title_short	φBO1E, a newly discovered lytic bacteriophage targeting carbapenemase-producing Klebsiella pneumoniae of the pandemic Clonal Group 258 clade II lineage
title_full	φBO1E, a newly discovered lytic bacteriophage targeting carbapenemase-producing Klebsiella pneumoniae of the pandemic Clonal Group 258 clade II lineage
title_fullStr	φBO1E, a newly discovered lytic bacteriophage targeting carbapenemase-producing Klebsiella pneumoniae of the pandemic Clonal Group 258 clade II lineage
title_full_unstemmed	φBO1E, a newly discovered lytic bacteriophage targeting carbapenemase-producing Klebsiella pneumoniae of the pandemic Clonal Group 258 clade II lineage
title_sort	φbo1e, a newly discovered lytic bacteriophage targeting carbapenemase-producing klebsiella pneumoniae of the pandemic clonal group 258 clade ii lineage
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/b3c5fb4bb510433088f5f3221a3b3021
work_keys_str_mv	AT marcomariadandrea phbo1eanewlydiscoveredlyticbacteriophagetargetingcarbapenemaseproducingklebsiellapneumoniaeofthepandemicclonalgroup258cladeiilineage AT pasqualemarmo phbo1eanewlydiscoveredlyticbacteriophagetargetingcarbapenemaseproducingklebsiellapneumoniaeofthepandemicclonalgroup258cladeiilineage AT luciahenricideangelis phbo1eanewlydiscoveredlyticbacteriophagetargetingcarbapenemaseproducingklebsiellapneumoniaeofthepandemicclonalgroup258cladeiilineage AT mattiapalmieri phbo1eanewlydiscoveredlyticbacteriophagetargetingcarbapenemaseproducingklebsiellapneumoniaeofthepandemicclonalgroup258cladeiilineage AT nagaiaciacci phbo1eanewlydiscoveredlyticbacteriophagetargetingcarbapenemaseproducingklebsiellapneumoniaeofthepandemicclonalgroup258cladeiilineage AT gustavodilallo phbo1eanewlydiscoveredlyticbacteriophagetargetingcarbapenemaseproducingklebsiellapneumoniaeofthepandemicclonalgroup258cladeiilineage AT elisadematte phbo1eanewlydiscoveredlyticbacteriophagetargetingcarbapenemaseproducingklebsiellapneumoniaeofthepandemicclonalgroup258cladeiilineage AT elisavannuccini phbo1eanewlydiscoveredlyticbacteriophagetargetingcarbapenemaseproducingklebsiellapneumoniaeofthepandemicclonalgroup258cladeiilineage AT pietrolupetti phbo1eanewlydiscoveredlyticbacteriophagetargetingcarbapenemaseproducingklebsiellapneumoniaeofthepandemicclonalgroup258cladeiilineage AT gianmariarossolini phbo1eanewlydiscoveredlyticbacteriophagetargetingcarbapenemaseproducingklebsiellapneumoniaeofthepandemicclonalgroup258cladeiilineage AT mariacristinathaller phbo1eanewlydiscoveredlyticbacteriophagetargetingcarbapenemaseproducingklebsiellapneumoniaeofthepandemicclonalgroup258cladeiilineage
_version_	1718394179314253824

φBO1E, a newly discovered lytic bacteriophage targeting carbapenemase-producing Klebsiella pneumoniae of the pandemic Clonal Group 258 clade II lineage

Ejemplares similares