Loss of P16 in Esophageal Adenocarcinoma Detected by Fluorescence in situ Hybridization and Immunohistochemistry

Molecular biology of esophageal adenocarcinoma (EAC) is not fully elucidated. The aim of this study was to assess the expression of cycle regulator and tumor suppressor p16 in esophageal adenocarcinoma. The expression of p16 at protein and gene level was investigated using immunohistochemistry and f...

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Autores principales:	Kotzev A., Kamenova M.
Formato:	article
Lenguaje:	EN
Publicado:	Sciendo 2017
Materias:	esophageal adenocarcinoma p16 immunohistochemistry fluorescence in situ hybridization Medicine R
Acceso en línea:	https://doaj.org/article/b3cda9adbbb74db3bbeaed71d44324a6
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spelling	oai:doaj.org-article:b3cda9adbbb74db3bbeaed71d44324a62021-12-02T17:31:48ZLoss of P16 in Esophageal Adenocarcinoma Detected by Fluorescence in situ Hybridization and Immunohistochemistry0324-175010.1515/amb-2017-0013https://doaj.org/article/b3cda9adbbb74db3bbeaed71d44324a62017-10-01T00:00:00Zhttps://doi.org/10.1515/amb-2017-0013https://doaj.org/toc/0324-1750Molecular biology of esophageal adenocarcinoma (EAC) is not fully elucidated. The aim of this study was to assess the expression of cycle regulator and tumor suppressor p16 in esophageal adenocarcinoma. The expression of p16 at protein and gene level was investigated using immunohistochemistry and fluorescence in situ hybridization in thirteen EAC specimens obtained by endoscopic biopsies and surgical resections. The mean age of enrolled patients was 62 years and a male predominance was observed. Loss of p16 protein expression was detected in 77% of the cases and loss of p16 gene was found in 69% of cases as hemizygous deletion was the most common. Significant correlation was found between the absence of p16 protein expression and p16 allelic loss. Cell cycle disturbances seem to play role in the EAC carcinogenesis and probably p16 gene deletions are connected with the loss of p16 protein expression.Kotzev A.Kamenova M.Sciendoarticleesophageal adenocarcinomap16immunohistochemistryfluorescence in situ hybridizationMedicineRENActa Medica Bulgarica, Vol 44, Iss 2, Pp 14-19 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	esophageal adenocarcinoma p16 immunohistochemistry fluorescence in situ hybridization Medicine R
spellingShingle	esophageal adenocarcinoma p16 immunohistochemistry fluorescence in situ hybridization Medicine R Kotzev A. Kamenova M. Loss of P16 in Esophageal Adenocarcinoma Detected by Fluorescence in situ Hybridization and Immunohistochemistry
description	Molecular biology of esophageal adenocarcinoma (EAC) is not fully elucidated. The aim of this study was to assess the expression of cycle regulator and tumor suppressor p16 in esophageal adenocarcinoma. The expression of p16 at protein and gene level was investigated using immunohistochemistry and fluorescence in situ hybridization in thirteen EAC specimens obtained by endoscopic biopsies and surgical resections. The mean age of enrolled patients was 62 years and a male predominance was observed. Loss of p16 protein expression was detected in 77% of the cases and loss of p16 gene was found in 69% of cases as hemizygous deletion was the most common. Significant correlation was found between the absence of p16 protein expression and p16 allelic loss. Cell cycle disturbances seem to play role in the EAC carcinogenesis and probably p16 gene deletions are connected with the loss of p16 protein expression.
format	article
author	Kotzev A. Kamenova M.
author_facet	Kotzev A. Kamenova M.
author_sort	Kotzev A.
title	Loss of P16 in Esophageal Adenocarcinoma Detected by Fluorescence in situ Hybridization and Immunohistochemistry
title_short	Loss of P16 in Esophageal Adenocarcinoma Detected by Fluorescence in situ Hybridization and Immunohistochemistry
title_full	Loss of P16 in Esophageal Adenocarcinoma Detected by Fluorescence in situ Hybridization and Immunohistochemistry
title_fullStr	Loss of P16 in Esophageal Adenocarcinoma Detected by Fluorescence in situ Hybridization and Immunohistochemistry
title_full_unstemmed	Loss of P16 in Esophageal Adenocarcinoma Detected by Fluorescence in situ Hybridization and Immunohistochemistry
title_sort	loss of p16 in esophageal adenocarcinoma detected by fluorescence in situ hybridization and immunohistochemistry
publisher	Sciendo
publishDate	2017
url	https://doaj.org/article/b3cda9adbbb74db3bbeaed71d44324a6
work_keys_str_mv	AT kotzeva lossofp16inesophagealadenocarcinomadetectedbyfluorescenceinsituhybridizationandimmunohistochemistry AT kamenovam lossofp16inesophagealadenocarcinomadetectedbyfluorescenceinsituhybridizationandimmunohistochemistry
_version_	1718380512373899264

Loss of P16 in Esophageal Adenocarcinoma Detected by Fluorescence in situ Hybridization and Immunohistochemistry

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