Humoral Response to Microbial Biomarkers in Rheumatoid Arthritis Patients

Background/Objective: Chronic humoral immune response against multiple microbial antigens may play a crucial role in the etiopathogenesis of rheumatoid arthritis (RA). We aimed to assess the prevalence and magnitude of antibody response against various bacterial and viral immunogen peptides in the s...

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Autores principales:	Seyedesomaye Jasemi, Gian Luca Erre, Maria Luisa Cadoni, Marco Bo, Leonardo A. Sechi
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	rheumatoid arthritis immune response <i>P. gingivalis</i> A. <i>actinomycetemcomitans</i> M. avium subspecies <i>paratuberculosis</i> Epstein–Barr virus Medicine R
Acceso en línea:	https://doaj.org/article/b3d7268dedd34401940bd97e78b20402
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spelling	oai:doaj.org-article:b3d7268dedd34401940bd97e78b204022021-11-11T17:46:18ZHumoral Response to Microbial Biomarkers in Rheumatoid Arthritis Patients10.3390/jcm102151532077-0383https://doaj.org/article/b3d7268dedd34401940bd97e78b204022021-11-01T00:00:00Zhttps://www.mdpi.com/2077-0383/10/21/5153https://doaj.org/toc/2077-0383Background/Objective: Chronic humoral immune response against multiple microbial antigens may play a crucial role in the etiopathogenesis of rheumatoid arthritis (RA). We aimed to assess the prevalence and magnitude of antibody response against various bacterial and viral immunogen peptides in the sera of RA patients compared with the general population. Methods: Polyclonal IgG antibodies (Abs) specific for peptides derived from <i>Porphyromonas gingivalis</i> (RgpA, Kpg), <i>Aggregatibacter <i>actinomycetemcomitans</i></i> (LtxA1, LtxA2), <i>Mycobacterium avium</i> subsp. <i>paratuberculosis</i> (MAP4027), Epstein–Barr virus (EBNA1, EBVBOLF), and human endogenous retrovirus (HERV-W env-su) were detected by ELISA in serum samples from 148 consecutive RA patients and 148 sex and age-matched healthy controls (HCs). In addition, the presence of a relationship between the positivity and the titer of antibodies and RA descriptors was explored by bivariate correlation analysis. Results: RA patients exhibit a higher prevalence of humoral immune response against all tested peptides compared to HCs with a statically significant difference for MAP4027 (30.4% vs. 10.1%), BOLF (25.7% vs. 8.1%), RgpA (24.3% vs. 9.4%), HERV W-env (20.3% vs. 9.4%), and EBNA1 (18.9% vs. 9.4%) peptides. Fifty-three (35.8%) out of 148 RA serum and 93 (62.8%) out of 148 HCs were negative for all pathogen-derived peptides. There was a significant correlation between OD values obtained by ELISA test against all peptides (<i>p</i> < 0.0001). We also found an increased titer and prevalence of Abs against LtxA1 and LtxA2 in seropositive vs. seronegative RF (<i>p</i> = 0.019, <i>p</i> = 0.018). Conclusion: This study demonstrates a significantly increased humoral response against multiple pathogens in patients with RA and implies that they could be an important factor in the pathogenesis of the disease. Therefore, the role of each individual pathogen in RA needs to be further investigated.Seyedesomaye JasemiGian Luca ErreMaria Luisa CadoniMarco BoLeonardo A. SechiMDPI AGarticlerheumatoid arthritisimmune response<i>P. gingivalis</i>A. <i>actinomycetemcomitans</i>M. avium subspecies <i>paratuberculosis</i>Epstein–Barr virusMedicineRENJournal of Clinical Medicine, Vol 10, Iss 5153, p 5153 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	rheumatoid arthritis immune response <i>P. gingivalis</i> A. <i>actinomycetemcomitans</i> M. avium subspecies <i>paratuberculosis</i> Epstein–Barr virus Medicine R
spellingShingle	rheumatoid arthritis immune response <i>P. gingivalis</i> A. <i>actinomycetemcomitans</i> M. avium subspecies <i>paratuberculosis</i> Epstein–Barr virus Medicine R Seyedesomaye Jasemi Gian Luca Erre Maria Luisa Cadoni Marco Bo Leonardo A. Sechi Humoral Response to Microbial Biomarkers in Rheumatoid Arthritis Patients
description	Background/Objective: Chronic humoral immune response against multiple microbial antigens may play a crucial role in the etiopathogenesis of rheumatoid arthritis (RA). We aimed to assess the prevalence and magnitude of antibody response against various bacterial and viral immunogen peptides in the sera of RA patients compared with the general population. Methods: Polyclonal IgG antibodies (Abs) specific for peptides derived from <i>Porphyromonas gingivalis</i> (RgpA, Kpg), <i>Aggregatibacter <i>actinomycetemcomitans</i></i> (LtxA1, LtxA2), <i>Mycobacterium avium</i> subsp. <i>paratuberculosis</i> (MAP4027), Epstein–Barr virus (EBNA1, EBVBOLF), and human endogenous retrovirus (HERV-W env-su) were detected by ELISA in serum samples from 148 consecutive RA patients and 148 sex and age-matched healthy controls (HCs). In addition, the presence of a relationship between the positivity and the titer of antibodies and RA descriptors was explored by bivariate correlation analysis. Results: RA patients exhibit a higher prevalence of humoral immune response against all tested peptides compared to HCs with a statically significant difference for MAP4027 (30.4% vs. 10.1%), BOLF (25.7% vs. 8.1%), RgpA (24.3% vs. 9.4%), HERV W-env (20.3% vs. 9.4%), and EBNA1 (18.9% vs. 9.4%) peptides. Fifty-three (35.8%) out of 148 RA serum and 93 (62.8%) out of 148 HCs were negative for all pathogen-derived peptides. There was a significant correlation between OD values obtained by ELISA test against all peptides (<i>p</i> < 0.0001). We also found an increased titer and prevalence of Abs against LtxA1 and LtxA2 in seropositive vs. seronegative RF (<i>p</i> = 0.019, <i>p</i> = 0.018). Conclusion: This study demonstrates a significantly increased humoral response against multiple pathogens in patients with RA and implies that they could be an important factor in the pathogenesis of the disease. Therefore, the role of each individual pathogen in RA needs to be further investigated.
format	article
author	Seyedesomaye Jasemi Gian Luca Erre Maria Luisa Cadoni Marco Bo Leonardo A. Sechi
author_facet	Seyedesomaye Jasemi Gian Luca Erre Maria Luisa Cadoni Marco Bo Leonardo A. Sechi
author_sort	Seyedesomaye Jasemi
title	Humoral Response to Microbial Biomarkers in Rheumatoid Arthritis Patients
title_short	Humoral Response to Microbial Biomarkers in Rheumatoid Arthritis Patients
title_full	Humoral Response to Microbial Biomarkers in Rheumatoid Arthritis Patients
title_fullStr	Humoral Response to Microbial Biomarkers in Rheumatoid Arthritis Patients
title_full_unstemmed	Humoral Response to Microbial Biomarkers in Rheumatoid Arthritis Patients
title_sort	humoral response to microbial biomarkers in rheumatoid arthritis patients
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/b3d7268dedd34401940bd97e78b20402
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Humoral Response to Microbial Biomarkers in Rheumatoid Arthritis Patients

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