Whole-genome comparison of two Campylobacter jejuni isolates of the same sequence type reveals multiple loci of different ancestral lineage.

Campylobacter jejuni ST-474 is the most important human enteric pathogen in New Zealand, and yet this genotype is rarely found elsewhere in the world. Insight into the evolution of this organism was gained by a whole genome comparison of two ST-474, flaA SVR-14 isolates and other available C. jejuni...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Patrick J Biggs, Paul Fearnhead, Grant Hotter, Vathsala Mohan, Julie Collins-Emerson, Errol Kwan, Thomas E Besser, Adrian Cookson, Philip E Carter, Nigel P French
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b3d7c49ca99842eba82f26af6b76da1f
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:b3d7c49ca99842eba82f26af6b76da1f
record_format	dspace
spelling	oai:doaj.org-article:b3d7c49ca99842eba82f26af6b76da1f2021-11-18T07:34:30ZWhole-genome comparison of two Campylobacter jejuni isolates of the same sequence type reveals multiple loci of different ancestral lineage.1932-620310.1371/journal.pone.0027121https://doaj.org/article/b3d7c49ca99842eba82f26af6b76da1f2011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22096527/?tool=EBIhttps://doaj.org/toc/1932-6203Campylobacter jejuni ST-474 is the most important human enteric pathogen in New Zealand, and yet this genotype is rarely found elsewhere in the world. Insight into the evolution of this organism was gained by a whole genome comparison of two ST-474, flaA SVR-14 isolates and other available C. jejuni isolates and genomes. The two isolates were collected from different sources, human (H22082) and retail poultry (P110b), at the same time and from the same geographical location. Solexa sequencing of each isolate resulted in ~1.659 Mb (H22082) and ~1.656 Mb (P110b) of assembled sequences within 28 (H22082) and 29 (P110b) contigs. We analysed 1502 genes for which we had sequences within both ST-474 isolates and within at least one of 11 C. jejuni reference genomes. Although 94.5% of genes were identical between the two ST-474 isolates, we identified 83 genes that differed by at least one nucleotide, including 55 genes with non-synonymous substitutions. These covered 101 kb and contained 672 point differences. We inferred that 22 (3.3%) of these differences were due to mutation and 650 (96.7%) were imported via recombination. Our analysis estimated 38 recombinant breakpoints within these 83 genes, which correspond to recombination events affecting at least 19 loci regions and gives a tract length estimate of ~2 kb. This includes a ~12 kb region displaying non-homologous recombination in one of the ST-474 genomes, with the insertion of two genes, including ykgC, a putative oxidoreductase, and a conserved hypothetical protein of unknown function. Furthermore, our analysis indicates that the source of this recombined DNA is more likely to have come from C. jejuni strains that are more closely related to ST-474. This suggests that the rates of recombination and mutation are similar in order of magnitude, but that recombination has been much more important for generating divergence between the two ST-474 isolates.Patrick J BiggsPaul FearnheadGrant HotterVathsala MohanJulie Collins-EmersonErrol KwanThomas E BesserAdrian CooksonPhilip E CarterNigel P FrenchPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 11, p e27121 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Patrick J Biggs Paul Fearnhead Grant Hotter Vathsala Mohan Julie Collins-Emerson Errol Kwan Thomas E Besser Adrian Cookson Philip E Carter Nigel P French Whole-genome comparison of two Campylobacter jejuni isolates of the same sequence type reveals multiple loci of different ancestral lineage.
description	Campylobacter jejuni ST-474 is the most important human enteric pathogen in New Zealand, and yet this genotype is rarely found elsewhere in the world. Insight into the evolution of this organism was gained by a whole genome comparison of two ST-474, flaA SVR-14 isolates and other available C. jejuni isolates and genomes. The two isolates were collected from different sources, human (H22082) and retail poultry (P110b), at the same time and from the same geographical location. Solexa sequencing of each isolate resulted in ~1.659 Mb (H22082) and ~1.656 Mb (P110b) of assembled sequences within 28 (H22082) and 29 (P110b) contigs. We analysed 1502 genes for which we had sequences within both ST-474 isolates and within at least one of 11 C. jejuni reference genomes. Although 94.5% of genes were identical between the two ST-474 isolates, we identified 83 genes that differed by at least one nucleotide, including 55 genes with non-synonymous substitutions. These covered 101 kb and contained 672 point differences. We inferred that 22 (3.3%) of these differences were due to mutation and 650 (96.7%) were imported via recombination. Our analysis estimated 38 recombinant breakpoints within these 83 genes, which correspond to recombination events affecting at least 19 loci regions and gives a tract length estimate of ~2 kb. This includes a ~12 kb region displaying non-homologous recombination in one of the ST-474 genomes, with the insertion of two genes, including ykgC, a putative oxidoreductase, and a conserved hypothetical protein of unknown function. Furthermore, our analysis indicates that the source of this recombined DNA is more likely to have come from C. jejuni strains that are more closely related to ST-474. This suggests that the rates of recombination and mutation are similar in order of magnitude, but that recombination has been much more important for generating divergence between the two ST-474 isolates.
format	article
author	Patrick J Biggs Paul Fearnhead Grant Hotter Vathsala Mohan Julie Collins-Emerson Errol Kwan Thomas E Besser Adrian Cookson Philip E Carter Nigel P French
author_facet	Patrick J Biggs Paul Fearnhead Grant Hotter Vathsala Mohan Julie Collins-Emerson Errol Kwan Thomas E Besser Adrian Cookson Philip E Carter Nigel P French
author_sort	Patrick J Biggs
title	Whole-genome comparison of two Campylobacter jejuni isolates of the same sequence type reveals multiple loci of different ancestral lineage.
title_short	Whole-genome comparison of two Campylobacter jejuni isolates of the same sequence type reveals multiple loci of different ancestral lineage.
title_full	Whole-genome comparison of two Campylobacter jejuni isolates of the same sequence type reveals multiple loci of different ancestral lineage.
title_fullStr	Whole-genome comparison of two Campylobacter jejuni isolates of the same sequence type reveals multiple loci of different ancestral lineage.
title_full_unstemmed	Whole-genome comparison of two Campylobacter jejuni isolates of the same sequence type reveals multiple loci of different ancestral lineage.
title_sort	whole-genome comparison of two campylobacter jejuni isolates of the same sequence type reveals multiple loci of different ancestral lineage.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/b3d7c49ca99842eba82f26af6b76da1f
work_keys_str_mv	AT patrickjbiggs wholegenomecomparisonoftwocampylobacterjejuniisolatesofthesamesequencetyperevealsmultiplelociofdifferentancestrallineage AT paulfearnhead wholegenomecomparisonoftwocampylobacterjejuniisolatesofthesamesequencetyperevealsmultiplelociofdifferentancestrallineage AT granthotter wholegenomecomparisonoftwocampylobacterjejuniisolatesofthesamesequencetyperevealsmultiplelociofdifferentancestrallineage AT vathsalamohan wholegenomecomparisonoftwocampylobacterjejuniisolatesofthesamesequencetyperevealsmultiplelociofdifferentancestrallineage AT juliecollinsemerson wholegenomecomparisonoftwocampylobacterjejuniisolatesofthesamesequencetyperevealsmultiplelociofdifferentancestrallineage AT errolkwan wholegenomecomparisonoftwocampylobacterjejuniisolatesofthesamesequencetyperevealsmultiplelociofdifferentancestrallineage AT thomasebesser wholegenomecomparisonoftwocampylobacterjejuniisolatesofthesamesequencetyperevealsmultiplelociofdifferentancestrallineage AT adriancookson wholegenomecomparisonoftwocampylobacterjejuniisolatesofthesamesequencetyperevealsmultiplelociofdifferentancestrallineage AT philipecarter wholegenomecomparisonoftwocampylobacterjejuniisolatesofthesamesequencetyperevealsmultiplelociofdifferentancestrallineage AT nigelpfrench wholegenomecomparisonoftwocampylobacterjejuniisolatesofthesamesequencetyperevealsmultiplelociofdifferentancestrallineage
_version_	1718423309296599040

Whole-genome comparison of two Campylobacter jejuni isolates of the same sequence type reveals multiple loci of different ancestral lineage.

Ejemplares similares