Trichostrongyloid nematodes in ruminants of northern Iran: prevalence and molecular analysis

Abstract Background This study was carried out to investigate the prevalence and analyze the molecular characteristics based on the internal transcribed spacer (ITS) 2 region of the ribosomal RNA (RNA) gene of trichostrongylid nematodes in different ruminants from Guilan province, northern of Iran....

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Autores principales:	Hedayat Hosseinnezhad, Meysam Sharifdini, Keyhan Ashrafi, Zahra Atrkar Roushan, Hamed Mirjalali, Behnaz Rahmati
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Trichostrongyloid nematodes Ruminants ITS2 Molecular analysis Iran Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/b3de44c7b14146f388f5cea3f53ad633
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spelling	oai:doaj.org-article:b3de44c7b14146f388f5cea3f53ad6332021-12-05T12:16:56ZTrichostrongyloid nematodes in ruminants of northern Iran: prevalence and molecular analysis10.1186/s12917-021-03086-31746-6148https://doaj.org/article/b3de44c7b14146f388f5cea3f53ad6332021-12-01T00:00:00Zhttps://doi.org/10.1186/s12917-021-03086-3https://doaj.org/toc/1746-6148Abstract Background This study was carried out to investigate the prevalence and analyze the molecular characteristics based on the internal transcribed spacer (ITS) 2 region of the ribosomal RNA (RNA) gene of trichostrongylid nematodes in different ruminants from Guilan province, northern of Iran. Methods The gastrointestinal tracts of 144 ruminants including 72 cattle, 59 sheep, and 13 goats were collected from an abattoir in Guilan province during July to September 2018. After isolation the helminths, male specimens were identified based on morphological parameters. PCR and partial sequencing of the ITS2 fragment were conducted. After phylogenetic analysis, the intraspecific and interspecific differences were calculated. Results The prevalence of total infections with the nematodes was 38.9, 74.6 and 84.6% among cattle, sheep and goats, respectively. Eleven species of trichostrongylid nematodes including Haemonchus contortus, Marshallagia marshalli, Trichostrongylus axei, T. colubriformis, T. vitrinus, Ostertagia trifurcata, Teladorsagia circumcincta, Marshallagia occidentalis, O. lyrata, O. ostertagi, and Cooperia punctate were recovered from the ruminants. The most prevalent trichostrongyloid nematodes in cattle, sheep and goats were O. ostertagi (26.4%), M. marshalli (64.4%) and T. circumcincta (69.2%), respectively. Phylogenetic tree was discriminative for Trichostrongylidae family, while phylogenetic analysis of the ITS2 gene represented low variations and no species identification of Haemonchidae and Cooperiidae families. Conclusions This study suggests the high prevalence and species diversity of trichostrongyloid nematodes in different ruminants, indicating the importance of implement antiparasitic strategies in north regions of Iran. As well, this study showed that the ITS2 fragment is not a discriminative marker for Haemonchidae and Cooperiidae families, and investigation of other genetic markers such as mitochondrial genes would be more valuable for better understanding of their phylogenetic relationships.Hedayat HosseinnezhadMeysam SharifdiniKeyhan AshrafiZahra Atrkar RoushanHamed MirjalaliBehnaz RahmatiBMCarticleTrichostrongyloid nematodesRuminantsITS2Molecular analysisIranVeterinary medicineSF600-1100ENBMC Veterinary Research, Vol 17, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Trichostrongyloid nematodes Ruminants ITS2 Molecular analysis Iran Veterinary medicine SF600-1100
spellingShingle	Trichostrongyloid nematodes Ruminants ITS2 Molecular analysis Iran Veterinary medicine SF600-1100 Hedayat Hosseinnezhad Meysam Sharifdini Keyhan Ashrafi Zahra Atrkar Roushan Hamed Mirjalali Behnaz Rahmati Trichostrongyloid nematodes in ruminants of northern Iran: prevalence and molecular analysis
description	Abstract Background This study was carried out to investigate the prevalence and analyze the molecular characteristics based on the internal transcribed spacer (ITS) 2 region of the ribosomal RNA (RNA) gene of trichostrongylid nematodes in different ruminants from Guilan province, northern of Iran. Methods The gastrointestinal tracts of 144 ruminants including 72 cattle, 59 sheep, and 13 goats were collected from an abattoir in Guilan province during July to September 2018. After isolation the helminths, male specimens were identified based on morphological parameters. PCR and partial sequencing of the ITS2 fragment were conducted. After phylogenetic analysis, the intraspecific and interspecific differences were calculated. Results The prevalence of total infections with the nematodes was 38.9, 74.6 and 84.6% among cattle, sheep and goats, respectively. Eleven species of trichostrongylid nematodes including Haemonchus contortus, Marshallagia marshalli, Trichostrongylus axei, T. colubriformis, T. vitrinus, Ostertagia trifurcata, Teladorsagia circumcincta, Marshallagia occidentalis, O. lyrata, O. ostertagi, and Cooperia punctate were recovered from the ruminants. The most prevalent trichostrongyloid nematodes in cattle, sheep and goats were O. ostertagi (26.4%), M. marshalli (64.4%) and T. circumcincta (69.2%), respectively. Phylogenetic tree was discriminative for Trichostrongylidae family, while phylogenetic analysis of the ITS2 gene represented low variations and no species identification of Haemonchidae and Cooperiidae families. Conclusions This study suggests the high prevalence and species diversity of trichostrongyloid nematodes in different ruminants, indicating the importance of implement antiparasitic strategies in north regions of Iran. As well, this study showed that the ITS2 fragment is not a discriminative marker for Haemonchidae and Cooperiidae families, and investigation of other genetic markers such as mitochondrial genes would be more valuable for better understanding of their phylogenetic relationships.
format	article
author	Hedayat Hosseinnezhad Meysam Sharifdini Keyhan Ashrafi Zahra Atrkar Roushan Hamed Mirjalali Behnaz Rahmati
author_facet	Hedayat Hosseinnezhad Meysam Sharifdini Keyhan Ashrafi Zahra Atrkar Roushan Hamed Mirjalali Behnaz Rahmati
author_sort	Hedayat Hosseinnezhad
title	Trichostrongyloid nematodes in ruminants of northern Iran: prevalence and molecular analysis
title_short	Trichostrongyloid nematodes in ruminants of northern Iran: prevalence and molecular analysis
title_full	Trichostrongyloid nematodes in ruminants of northern Iran: prevalence and molecular analysis
title_fullStr	Trichostrongyloid nematodes in ruminants of northern Iran: prevalence and molecular analysis
title_full_unstemmed	Trichostrongyloid nematodes in ruminants of northern Iran: prevalence and molecular analysis
title_sort	trichostrongyloid nematodes in ruminants of northern iran: prevalence and molecular analysis
publisher	BMC
publishDate	2021
url	https://doaj.org/article/b3de44c7b14146f388f5cea3f53ad633
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Trichostrongyloid nematodes in ruminants of northern Iran: prevalence and molecular analysis

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