Superpulsed low-level laser therapy protects skeletal muscle of mdx mice against damage, inflammation and morphological changes delaying dystrophy progression.

<h4>Aim</h4>To evaluate the effects of preventive treatment with low-level laser therapy (LLLT) on progression of dystrophy in mdx mice.<h4>Methods</h4>Ten animals were randomly divided into 2 experimental groups treated with superpulsed LLLT (904 nm, 15 mW, 700 Hz, 1 J) or p...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ernesto Cesar Pinto Leal-Junior, Patrícia de Almeida, Shaiane Silva Tomazoni, Paulo de Tarso Camillo de Carvalho, Rodrigo Álvaro Brandão Lopes-Martins, Lucio Frigo, Jon Joensen, Mark I Johnson, Jan Magnus Bjordal
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b3e1ea8339b643c4a9c76828962ee5cf
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:b3e1ea8339b643c4a9c76828962ee5cf
record_format	dspace
spelling	oai:doaj.org-article:b3e1ea8339b643c4a9c76828962ee5cf2021-11-18T08:29:40ZSuperpulsed low-level laser therapy protects skeletal muscle of mdx mice against damage, inflammation and morphological changes delaying dystrophy progression.1932-620310.1371/journal.pone.0089453https://doaj.org/article/b3e1ea8339b643c4a9c76828962ee5cf2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24599021/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Aim</h4>To evaluate the effects of preventive treatment with low-level laser therapy (LLLT) on progression of dystrophy in mdx mice.<h4>Methods</h4>Ten animals were randomly divided into 2 experimental groups treated with superpulsed LLLT (904 nm, 15 mW, 700 Hz, 1 J) or placebo-LLLT at one point overlying the tibialis anterior muscle (bilaterally) 5 times per week for 14 weeks (from 6th to 20th week of age). Morphological changes, creatine kinase (CK) activity and mRNA gene expression were assessed in animals at 20th week of age.<h4>Results</h4>Animals treated with LLLT showed very few morphological changes in skeletal muscle, with less atrophy and fibrosis than animals treated with placebo-LLLT. CK was significantly lower (p=0.0203) in animals treated with LLLT (864.70 U.l-1, SEM 226.10) than placebo (1708.00 U.l-1, SEM 184.60). mRNA gene expression of inflammatory markers was significantly decreased by treatment with LLLT (p<0.05): TNF-α (placebo-control=0.51 µg/µl [SEM 0.12], - LLLT=0.048 µg/µl [SEM 0.01]), IL-1β (placebo-control=2.292 µg/µl [SEM 0.74], - LLLT=0.12 µg/µl [SEM 0.03]), IL-6 (placebo-control=3.946 µg/µl [SEM 0.98], - LLLT=0.854 µg/µl [SEM 0.33]), IL-10 (placebo-control=1.116 µg/µl [SEM 0.22], - LLLT=0.352 µg/µl [SEM 0.15]), and COX-2 (placebo-control=4.984 µg/µl [SEM 1.18], LLLT=1.470 µg/µl [SEM 0.73]).<h4>Conclusion</h4>Irradiation of superpulsed LLLT on successive days five times per week for 14 weeks decreased morphological changes, skeletal muscle damage and inflammation in mdx mice. This indicates that LLLT has potential to decrease progression of Duchenne muscular dystrophy.Ernesto Cesar Pinto Leal-JuniorPatrícia de AlmeidaShaiane Silva TomazoniPaulo de Tarso Camillo de CarvalhoRodrigo Álvaro Brandão Lopes-MartinsLucio FrigoJon JoensenMark I JohnsonJan Magnus BjordalPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 3, p e89453 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ernesto Cesar Pinto Leal-Junior Patrícia de Almeida Shaiane Silva Tomazoni Paulo de Tarso Camillo de Carvalho Rodrigo Álvaro Brandão Lopes-Martins Lucio Frigo Jon Joensen Mark I Johnson Jan Magnus Bjordal Superpulsed low-level laser therapy protects skeletal muscle of mdx mice against damage, inflammation and morphological changes delaying dystrophy progression.
description	<h4>Aim</h4>To evaluate the effects of preventive treatment with low-level laser therapy (LLLT) on progression of dystrophy in mdx mice.<h4>Methods</h4>Ten animals were randomly divided into 2 experimental groups treated with superpulsed LLLT (904 nm, 15 mW, 700 Hz, 1 J) or placebo-LLLT at one point overlying the tibialis anterior muscle (bilaterally) 5 times per week for 14 weeks (from 6th to 20th week of age). Morphological changes, creatine kinase (CK) activity and mRNA gene expression were assessed in animals at 20th week of age.<h4>Results</h4>Animals treated with LLLT showed very few morphological changes in skeletal muscle, with less atrophy and fibrosis than animals treated with placebo-LLLT. CK was significantly lower (p=0.0203) in animals treated with LLLT (864.70 U.l-1, SEM 226.10) than placebo (1708.00 U.l-1, SEM 184.60). mRNA gene expression of inflammatory markers was significantly decreased by treatment with LLLT (p<0.05): TNF-α (placebo-control=0.51 µg/µl [SEM 0.12], - LLLT=0.048 µg/µl [SEM 0.01]), IL-1β (placebo-control=2.292 µg/µl [SEM 0.74], - LLLT=0.12 µg/µl [SEM 0.03]), IL-6 (placebo-control=3.946 µg/µl [SEM 0.98], - LLLT=0.854 µg/µl [SEM 0.33]), IL-10 (placebo-control=1.116 µg/µl [SEM 0.22], - LLLT=0.352 µg/µl [SEM 0.15]), and COX-2 (placebo-control=4.984 µg/µl [SEM 1.18], LLLT=1.470 µg/µl [SEM 0.73]).<h4>Conclusion</h4>Irradiation of superpulsed LLLT on successive days five times per week for 14 weeks decreased morphological changes, skeletal muscle damage and inflammation in mdx mice. This indicates that LLLT has potential to decrease progression of Duchenne muscular dystrophy.
format	article
author	Ernesto Cesar Pinto Leal-Junior Patrícia de Almeida Shaiane Silva Tomazoni Paulo de Tarso Camillo de Carvalho Rodrigo Álvaro Brandão Lopes-Martins Lucio Frigo Jon Joensen Mark I Johnson Jan Magnus Bjordal
author_facet	Ernesto Cesar Pinto Leal-Junior Patrícia de Almeida Shaiane Silva Tomazoni Paulo de Tarso Camillo de Carvalho Rodrigo Álvaro Brandão Lopes-Martins Lucio Frigo Jon Joensen Mark I Johnson Jan Magnus Bjordal
author_sort	Ernesto Cesar Pinto Leal-Junior
title	Superpulsed low-level laser therapy protects skeletal muscle of mdx mice against damage, inflammation and morphological changes delaying dystrophy progression.
title_short	Superpulsed low-level laser therapy protects skeletal muscle of mdx mice against damage, inflammation and morphological changes delaying dystrophy progression.
title_full	Superpulsed low-level laser therapy protects skeletal muscle of mdx mice against damage, inflammation and morphological changes delaying dystrophy progression.
title_fullStr	Superpulsed low-level laser therapy protects skeletal muscle of mdx mice against damage, inflammation and morphological changes delaying dystrophy progression.
title_full_unstemmed	Superpulsed low-level laser therapy protects skeletal muscle of mdx mice against damage, inflammation and morphological changes delaying dystrophy progression.
title_sort	superpulsed low-level laser therapy protects skeletal muscle of mdx mice against damage, inflammation and morphological changes delaying dystrophy progression.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/b3e1ea8339b643c4a9c76828962ee5cf
work_keys_str_mv	AT ernestocesarpintolealjunior superpulsedlowlevellasertherapyprotectsskeletalmuscleofmdxmiceagainstdamageinflammationandmorphologicalchangesdelayingdystrophyprogression AT patriciadealmeida superpulsedlowlevellasertherapyprotectsskeletalmuscleofmdxmiceagainstdamageinflammationandmorphologicalchangesdelayingdystrophyprogression AT shaianesilvatomazoni superpulsedlowlevellasertherapyprotectsskeletalmuscleofmdxmiceagainstdamageinflammationandmorphologicalchangesdelayingdystrophyprogression AT paulodetarsocamillodecarvalho superpulsedlowlevellasertherapyprotectsskeletalmuscleofmdxmiceagainstdamageinflammationandmorphologicalchangesdelayingdystrophyprogression AT rodrigoalvarobrandaolopesmartins superpulsedlowlevellasertherapyprotectsskeletalmuscleofmdxmiceagainstdamageinflammationandmorphologicalchangesdelayingdystrophyprogression AT luciofrigo superpulsedlowlevellasertherapyprotectsskeletalmuscleofmdxmiceagainstdamageinflammationandmorphologicalchangesdelayingdystrophyprogression AT jonjoensen superpulsedlowlevellasertherapyprotectsskeletalmuscleofmdxmiceagainstdamageinflammationandmorphologicalchangesdelayingdystrophyprogression AT markijohnson superpulsedlowlevellasertherapyprotectsskeletalmuscleofmdxmiceagainstdamageinflammationandmorphologicalchangesdelayingdystrophyprogression AT janmagnusbjordal superpulsedlowlevellasertherapyprotectsskeletalmuscleofmdxmiceagainstdamageinflammationandmorphologicalchangesdelayingdystrophyprogression
_version_	1718421765450891264

Superpulsed low-level laser therapy protects skeletal muscle of mdx mice against damage, inflammation and morphological changes delaying dystrophy progression.

Ejemplares similares