Transcriptional trajectories of anther development provide candidates for engineering male fertility in sorghum

Abstract Sorghum is a self-pollinated crop with multiple economic uses as cereal, forage, and biofuel feedstock. Hybrid breeding is a cornerstone for sorghum improvement strategies that currently relies on cytoplasmic male sterile lines. To engineer genic male sterility, it is imperative to examine...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Namrata Dhaka, Kushagra Krishnan, Manu Kandpal, Ira Vashisht, Madan Pal, Manoj Kumar Sharma, Rita Sharma
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b3ebf7c0690f4fa2b8ab5ca9b6fc45d8
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:b3ebf7c0690f4fa2b8ab5ca9b6fc45d8
record_format	dspace
spelling	oai:doaj.org-article:b3ebf7c0690f4fa2b8ab5ca9b6fc45d82021-12-02T15:23:47ZTranscriptional trajectories of anther development provide candidates for engineering male fertility in sorghum10.1038/s41598-020-57717-02045-2322https://doaj.org/article/b3ebf7c0690f4fa2b8ab5ca9b6fc45d82020-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-57717-0https://doaj.org/toc/2045-2322Abstract Sorghum is a self-pollinated crop with multiple economic uses as cereal, forage, and biofuel feedstock. Hybrid breeding is a cornerstone for sorghum improvement strategies that currently relies on cytoplasmic male sterile lines. To engineer genic male sterility, it is imperative to examine the genetic components regulating anther/pollen development in sorghum. To this end, we have performed transcriptomic analysis from three temporal stages of developing anthers that correspond to meiotic, microspore and mature pollen stages. A total of 5286 genes were differentially regulated among the three anther stages with 890 of them exhibiting anther-preferential expression. Differentially expressed genes could be clubbed into seven distinct developmental trajectories using K-means clustering. Pathway mapping revealed that genes involved in cell cycle, DNA repair, regulation of transcription, brassinosteroid and auxin biosynthesis/signalling exhibit peak expression in meiotic anthers, while those regulating abiotic stress, carbohydrate metabolism, and transport were enriched in microspore stage. Conversely, genes associated with protein degradation, post-translational modifications, cell wall biosynthesis/modifications, abscisic acid, ethylene, cytokinin and jasmonic acid biosynthesis/signalling were highly expressed in mature pollen stage. High concurrence in transcriptional dynamics and cis-regulatory elements of differentially expressed genes in rice and sorghum confirmed conserved developmental pathways regulating anther development across species. Comprehensive literature survey in conjunction with orthology analysis and anther-preferential accumulation enabled shortlisting of 21 prospective candidates for in-depth characterization and engineering male fertility in sorghum.Namrata DhakaKushagra KrishnanManu KandpalIra VashishtMadan PalManoj Kumar SharmaRita SharmaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-16 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Namrata Dhaka Kushagra Krishnan Manu Kandpal Ira Vashisht Madan Pal Manoj Kumar Sharma Rita Sharma Transcriptional trajectories of anther development provide candidates for engineering male fertility in sorghum
description	Abstract Sorghum is a self-pollinated crop with multiple economic uses as cereal, forage, and biofuel feedstock. Hybrid breeding is a cornerstone for sorghum improvement strategies that currently relies on cytoplasmic male sterile lines. To engineer genic male sterility, it is imperative to examine the genetic components regulating anther/pollen development in sorghum. To this end, we have performed transcriptomic analysis from three temporal stages of developing anthers that correspond to meiotic, microspore and mature pollen stages. A total of 5286 genes were differentially regulated among the three anther stages with 890 of them exhibiting anther-preferential expression. Differentially expressed genes could be clubbed into seven distinct developmental trajectories using K-means clustering. Pathway mapping revealed that genes involved in cell cycle, DNA repair, regulation of transcription, brassinosteroid and auxin biosynthesis/signalling exhibit peak expression in meiotic anthers, while those regulating abiotic stress, carbohydrate metabolism, and transport were enriched in microspore stage. Conversely, genes associated with protein degradation, post-translational modifications, cell wall biosynthesis/modifications, abscisic acid, ethylene, cytokinin and jasmonic acid biosynthesis/signalling were highly expressed in mature pollen stage. High concurrence in transcriptional dynamics and cis-regulatory elements of differentially expressed genes in rice and sorghum confirmed conserved developmental pathways regulating anther development across species. Comprehensive literature survey in conjunction with orthology analysis and anther-preferential accumulation enabled shortlisting of 21 prospective candidates for in-depth characterization and engineering male fertility in sorghum.
format	article
author	Namrata Dhaka Kushagra Krishnan Manu Kandpal Ira Vashisht Madan Pal Manoj Kumar Sharma Rita Sharma
author_facet	Namrata Dhaka Kushagra Krishnan Manu Kandpal Ira Vashisht Madan Pal Manoj Kumar Sharma Rita Sharma
author_sort	Namrata Dhaka
title	Transcriptional trajectories of anther development provide candidates for engineering male fertility in sorghum
title_short	Transcriptional trajectories of anther development provide candidates for engineering male fertility in sorghum
title_full	Transcriptional trajectories of anther development provide candidates for engineering male fertility in sorghum
title_fullStr	Transcriptional trajectories of anther development provide candidates for engineering male fertility in sorghum
title_full_unstemmed	Transcriptional trajectories of anther development provide candidates for engineering male fertility in sorghum
title_sort	transcriptional trajectories of anther development provide candidates for engineering male fertility in sorghum
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/b3ebf7c0690f4fa2b8ab5ca9b6fc45d8
work_keys_str_mv	AT namratadhaka transcriptionaltrajectoriesofantherdevelopmentprovidecandidatesforengineeringmalefertilityinsorghum AT kushagrakrishnan transcriptionaltrajectoriesofantherdevelopmentprovidecandidatesforengineeringmalefertilityinsorghum AT manukandpal transcriptionaltrajectoriesofantherdevelopmentprovidecandidatesforengineeringmalefertilityinsorghum AT iravashisht transcriptionaltrajectoriesofantherdevelopmentprovidecandidatesforengineeringmalefertilityinsorghum AT madanpal transcriptionaltrajectoriesofantherdevelopmentprovidecandidatesforengineeringmalefertilityinsorghum AT manojkumarsharma transcriptionaltrajectoriesofantherdevelopmentprovidecandidatesforengineeringmalefertilityinsorghum AT ritasharma transcriptionaltrajectoriesofantherdevelopmentprovidecandidatesforengineeringmalefertilityinsorghum
_version_	1718387286224142336

Transcriptional trajectories of anther development provide candidates for engineering male fertility in sorghum

Ejemplares similares