Genes found essential in other mycoplasmas are dispensable in Mycoplasma bovis.

Mycoplasmas are regarded to be useful models for studying the minimum genetic complement required for independent survival of an organism. Mycoplasma bovis is a globally distributed pathogen causing pneumonia, mastitis, arthritis, otitis media and reproductive tract disease, and genome sequences of...

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Autores principales:	Shukriti Sharma, Philip F Markham, Glenn F Browning
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b3ef9f431969425bac00e161cff41d62
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spelling	oai:doaj.org-article:b3ef9f431969425bac00e161cff41d622021-11-18T08:17:05ZGenes found essential in other mycoplasmas are dispensable in Mycoplasma bovis.1932-620310.1371/journal.pone.0097100https://doaj.org/article/b3ef9f431969425bac00e161cff41d622014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24897538/?tool=EBIhttps://doaj.org/toc/1932-6203Mycoplasmas are regarded to be useful models for studying the minimum genetic complement required for independent survival of an organism. Mycoplasma bovis is a globally distributed pathogen causing pneumonia, mastitis, arthritis, otitis media and reproductive tract disease, and genome sequences of three strains, the type strain PG45 and two strains isolated in China, have been published. In this study, several Tn4001 based transposon constructs were generated and used to create a M. bovis PG45 insertional mutant library. Direct genome sequencing of 319 independent insertions detected disruptions in 129 genes in M. bovis, 48 of which had homologues in Mycoplasma mycoides subspecies mycoides SC and 99 of which had homologues in Mycoplasma agalactiae. Sixteen genes found to be essential in previous studies on other mycoplasma species were found to be dispensable. Five of these genes have previously been predicted to be part of the core set of 153 essential genes in mycoplasmas. Thus this study has extended the list of non-essential genes of mycoplasmas from that previously generated by studies in other species.Shukriti SharmaPhilip F MarkhamGlenn F BrowningPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 6, p e97100 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shukriti Sharma Philip F Markham Glenn F Browning Genes found essential in other mycoplasmas are dispensable in Mycoplasma bovis.
description	Mycoplasmas are regarded to be useful models for studying the minimum genetic complement required for independent survival of an organism. Mycoplasma bovis is a globally distributed pathogen causing pneumonia, mastitis, arthritis, otitis media and reproductive tract disease, and genome sequences of three strains, the type strain PG45 and two strains isolated in China, have been published. In this study, several Tn4001 based transposon constructs were generated and used to create a M. bovis PG45 insertional mutant library. Direct genome sequencing of 319 independent insertions detected disruptions in 129 genes in M. bovis, 48 of which had homologues in Mycoplasma mycoides subspecies mycoides SC and 99 of which had homologues in Mycoplasma agalactiae. Sixteen genes found to be essential in previous studies on other mycoplasma species were found to be dispensable. Five of these genes have previously been predicted to be part of the core set of 153 essential genes in mycoplasmas. Thus this study has extended the list of non-essential genes of mycoplasmas from that previously generated by studies in other species.
format	article
author	Shukriti Sharma Philip F Markham Glenn F Browning
author_facet	Shukriti Sharma Philip F Markham Glenn F Browning
author_sort	Shukriti Sharma
title	Genes found essential in other mycoplasmas are dispensable in Mycoplasma bovis.
title_short	Genes found essential in other mycoplasmas are dispensable in Mycoplasma bovis.
title_full	Genes found essential in other mycoplasmas are dispensable in Mycoplasma bovis.
title_fullStr	Genes found essential in other mycoplasmas are dispensable in Mycoplasma bovis.
title_full_unstemmed	Genes found essential in other mycoplasmas are dispensable in Mycoplasma bovis.
title_sort	genes found essential in other mycoplasmas are dispensable in mycoplasma bovis.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/b3ef9f431969425bac00e161cff41d62
work_keys_str_mv	AT shukritisharma genesfoundessentialinothermycoplasmasaredispensableinmycoplasmabovis AT philipfmarkham genesfoundessentialinothermycoplasmasaredispensableinmycoplasmabovis AT glennfbrowning genesfoundessentialinothermycoplasmasaredispensableinmycoplasmabovis
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Genes found essential in other mycoplasmas are dispensable in Mycoplasma bovis.

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