Fabrication of ultra-small monolayer graphene quantum dots by pyrolysis of trisodium citrate for fluorescent cell imaging

Guo-Lin Hong,1 Hai-Ling Zhao,2,3 Hao-Hua Deng,3 Hui-Jing Yang,4 Hua-Ping Peng,3 Yin-Huan Liu,5 Wei Chen3 1Department of Laboratory Medicine, The First Affiliated Hospital of Xiamen University, Xiamen 361003, People’s Republic of China; 2School of Public Health, Xiamen University, Xiamen 3...

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Main Authors:	Hong GL, Zhao HL, Deng HH, Yang HJ, Peng HP, Liu YH, Chen W
Format:	article
Language:	EN
Published:	Dove Medical Press 2018
Subjects:	graphene quantum dots fluorescence cell imaging trisodium citrate pyrolysis Medicine (General) R5-920
Online Access:	https://doaj.org/article/b3f6e50be8554d56926e5d94a27a25c5
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spelling	oai:doaj.org-article:b3f6e50be8554d56926e5d94a27a25c52021-12-02T06:51:14ZFabrication of ultra-small monolayer graphene quantum dots by pyrolysis of trisodium citrate for fluorescent cell imaging1178-2013https://doaj.org/article/b3f6e50be8554d56926e5d94a27a25c52018-08-01T00:00:00Zhttps://www.dovepress.com/fabrication-of-ultra-small-monolayer-graphene-quantum-dots-by-pyrolysi-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Guo-Lin Hong,1 Hai-Ling Zhao,2,3 Hao-Hua Deng,3 Hui-Jing Yang,4 Hua-Ping Peng,3 Yin-Huan Liu,5 Wei Chen3 1Department of Laboratory Medicine, The First Affiliated Hospital of Xiamen University, Xiamen 361003, People’s Republic of China; 2School of Public Health, Xiamen University, Xiamen 361102, People’s Republic of China; 3Department of Pharmaceutical Analysis, Higher Educational Key Laboratory for Nano Biomedical Technology of Fujian Province, Fujian Medical University, Fuzhou 350004, People’s Republic of China; 4Department of Laboratory Medicine, Fujian Medical University, Fuzhou 350004, People’s Republic of China; 5Department of Laboratory Medicine, The Affiliated Fuzhou Second Hospital of Xiamen University, Fuzhou 350007, People’s Republic of China Background: The preparation and biological applications of ultra-small graphene quantum dots (GQDs) with accurate-controlled size are of great significance. Methods: Here in, we report a novel procedure involving pyrolysis of trisodium citrate and subsequent ultrafiltration for fabricating monolayer GQDs with ultra-small lateral size (1.3±0.5 nm). Results: The GQDs exhibit blue photoluminescence with peak position independent of excitation wavelength. The quantum yield of GQDs is measured to be 3.6%, and the average fluorescence lifetime is 2.78 ns. Conclusion: Because of high stability and low toxicity, GQDs are demonstrated to be excellent bioimaging agents. The ultra-small GQDs can not only distribute in the cytoplasm but also penetrate into the nuclei. We ensure that this work will add a new dimension to the application of graphene materials for nanomedicine. Keywords: graphene quantum dots, fluorescence, cell imaging, trisodium citrate, pyrolysisHong GLZhao HLDeng HHYang HJPeng HPLiu YHChen WDove Medical Pressarticlegraphene quantum dotsfluorescencecell imagingtrisodium citratepyrolysisMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 13, Pp 4807-4815 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	graphene quantum dots fluorescence cell imaging trisodium citrate pyrolysis Medicine (General) R5-920
spellingShingle	graphene quantum dots fluorescence cell imaging trisodium citrate pyrolysis Medicine (General) R5-920 Hong GL Zhao HL Deng HH Yang HJ Peng HP Liu YH Chen W Fabrication of ultra-small monolayer graphene quantum dots by pyrolysis of trisodium citrate for fluorescent cell imaging
description	Guo-Lin Hong,1 Hai-Ling Zhao,2,3 Hao-Hua Deng,3 Hui-Jing Yang,4 Hua-Ping Peng,3 Yin-Huan Liu,5 Wei Chen3 1Department of Laboratory Medicine, The First Affiliated Hospital of Xiamen University, Xiamen 361003, People’s Republic of China; 2School of Public Health, Xiamen University, Xiamen 361102, People’s Republic of China; 3Department of Pharmaceutical Analysis, Higher Educational Key Laboratory for Nano Biomedical Technology of Fujian Province, Fujian Medical University, Fuzhou 350004, People’s Republic of China; 4Department of Laboratory Medicine, Fujian Medical University, Fuzhou 350004, People’s Republic of China; 5Department of Laboratory Medicine, The Affiliated Fuzhou Second Hospital of Xiamen University, Fuzhou 350007, People’s Republic of China Background: The preparation and biological applications of ultra-small graphene quantum dots (GQDs) with accurate-controlled size are of great significance. Methods: Here in, we report a novel procedure involving pyrolysis of trisodium citrate and subsequent ultrafiltration for fabricating monolayer GQDs with ultra-small lateral size (1.3±0.5 nm). Results: The GQDs exhibit blue photoluminescence with peak position independent of excitation wavelength. The quantum yield of GQDs is measured to be 3.6%, and the average fluorescence lifetime is 2.78 ns. Conclusion: Because of high stability and low toxicity, GQDs are demonstrated to be excellent bioimaging agents. The ultra-small GQDs can not only distribute in the cytoplasm but also penetrate into the nuclei. We ensure that this work will add a new dimension to the application of graphene materials for nanomedicine. Keywords: graphene quantum dots, fluorescence, cell imaging, trisodium citrate, pyrolysis
format	article
author	Hong GL Zhao HL Deng HH Yang HJ Peng HP Liu YH Chen W
author_facet	Hong GL Zhao HL Deng HH Yang HJ Peng HP Liu YH Chen W
author_sort	Hong GL
title	Fabrication of ultra-small monolayer graphene quantum dots by pyrolysis of trisodium citrate for fluorescent cell imaging
title_short	Fabrication of ultra-small monolayer graphene quantum dots by pyrolysis of trisodium citrate for fluorescent cell imaging
title_full	Fabrication of ultra-small monolayer graphene quantum dots by pyrolysis of trisodium citrate for fluorescent cell imaging
title_fullStr	Fabrication of ultra-small monolayer graphene quantum dots by pyrolysis of trisodium citrate for fluorescent cell imaging
title_full_unstemmed	Fabrication of ultra-small monolayer graphene quantum dots by pyrolysis of trisodium citrate for fluorescent cell imaging
title_sort	fabrication of ultra-small monolayer graphene quantum dots by pyrolysis of trisodium citrate for fluorescent cell imaging
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/b3f6e50be8554d56926e5d94a27a25c5
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Fabrication of ultra-small monolayer graphene quantum dots by pyrolysis of trisodium citrate for fluorescent cell imaging

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